LOCUS       Exported     3490 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3490
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3476
                     /label="guaB"
                     /note="Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth."
                     /translation="MWESKFSKEGLTFDDVLLVPAKSEVLPRDVDLSVELTKTLKLNIPVISAGMDTVTESAMAIAMARQGGLGIIHKNMSIEQQAEQVDKVKRSERGVITNPFFLTPDHQVFDAEHLMGKYRISGVPIVNNEEDQKLVGIITNRDLRFISDYSMKISDVMTKEELVTASVGTTLDEAEKILQKHKIEKLPLVDDQNKLKGLITIKDIEKVIEFPNSSKDIHGRLIVGAAVGVTGDTMTRVKKLVEANVDVIVIDTAHGHSQGVLNTVTKIRETYPELNIIAGNVATAEATRALIEAGADVVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHGKTIIADGGIKFSGDITKALAAGGHAVMLGSLLAGTSESPGETEIYQGRRFKVYRGMGSVAAMEKGSKDRYFQEENKKFVPEGIEGRTPYKGPVEETVYQLVGGLRSGMGYCGSKDLRALREEAQFIRMTGAGLRESHPHDVQITKESPNYTIS"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTGGGAGAG CAAGTTTTCG AAAGAAGGCC
     2041 TGACCTTTGA TGATGTGCTG TTGGTCCCGG CAAAGAGCGA AGTGCTGCCG CGTGATGTTG
     2101 ATCTGAGCGT AGAACTGACC AAAACCCTGA AGCTGAATAT TCCGGTGATC AGCGCGGGTA
     2161 TGGATACGGT GACCGAGTCC GCAATGGCGA TCGCCATGGC TCGCCAGGGC GGCCTTGGTA
     2221 TTATTCATAA AAACATGAGC ATTGAACAAC AAGCAGAACA GGTCGACAAA GTGAAACGTT
     2281 CTGAGCGTGG CGTCATTACC AACCCATTCT TCCTGACCCC AGACCATCAA GTCTTTGATG
     2341 CGGAACACCT GATGGGCAAA TACCGTATCA GCGGCGTCCC GATTGTAAAC AATGAAGAGG
     2401 ACCAGAAGCT GGTCGGCATT ATTACTAACC GTGATCTGCG TTTCATTAGC GACTACAGCA
     2461 TGAAGATCTC GGATGTAATG ACCAAGGAGG AACTGGTTAC CGCGTCAGTT GGCACCACCC
     2521 TGGACGAGGC GGAAAAAATC TTGCAGAAAC ATAAGATTGA GAAGCTGCCG CTGGTCGACG
     2581 ACCAGAACAA GCTGAAAGGT CTGATTACCA TCAAGGACAT CGAGAAAGTG ATCGAGTTCC
     2641 CGAATAGCAG CAAGGATATC CACGGTCGTC TGATCGTTGG CGCCGCGGTG GGCGTCACGG
     2701 GCGACACCAT GACCCGCGTC AAAAAACTGG TGGAAGCGAA TGTCGATGTC ATCGTTATCG
     2761 ACACCGCCCA CGGCCACTCT CAAGGTGTCC TGAATACCGT TACGAAAATT CGTGAAACCT
     2821 ATCCGGAGCT GAATATTATC GCAGGCAACG TGGCAACGGC GGAGGCGACC CGCGCATTGA
     2881 TTGAGGCTGG CGCTGATGTC GTGAAGGTCG GCATCGGTCC GGGCAGCATC TGCACGACGC
     2941 GCGTCGTCGC GGGTGTTGGT GTTCCGCAAA TCACCGCCAT TTACGATTGT GCGACGGAGG
     3001 CACGTAAACA TGGTAAGACC ATTATTGCGG ATGGTGGCAT TAAATTTTCC GGCGATATCA
     3061 CGAAAGCGCT GGCGGCGGGT GGTCACGCCG TTATGCTGGG TAGCTTGCTG GCCGGTACCT
     3121 CTGAAAGCCC GGGTGAAACC GAGATCTATC AAGGTCGTCG TTTCAAGGTG TATCGCGGTA
     3181 TGGGCAGCGT GGCGGCTATG GAGAAGGGTA GCAAAGACCG CTACTTTCAA GAGGAGAACA
     3241 AGAAGTTCGT GCCTGAGGGC ATCGAGGGTC GTACTCCGTA CAAAGGCCCA GTGGAAGAAA
     3301 CGGTGTACCA ACTGGTGGGC GGTCTGCGCA GCGGCATGGG CTACTGCGGT AGCAAGGATC
     3361 TGCGTGCGCT GCGCGAAGAG GCACAGTTTA TCAGAATGAC GGGTGCTGGT CTGCGCGAGA
     3421 GCCACCCGCA TGACGTCCAA ATTACGAAGG AAAGCCCGAA CTATACCATC TCCTGAAGAG
     3481 CTTAGAGACC
//