LOCUS       Exported     3712 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3712
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1750..1797
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3645..3661
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3662..3693
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3711
                     /note="DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria."
                     /translation="MSYQALYRVFRPQRFEDVVGQEHITKTLQNALLQKKFSHAYLFSGPRGTGKTSAAKIFAKAVNCEHAPVDEPCNECAACKGITNGSISDVIEIDAASNNGVDEIRDIRDKVKFAPSAVTYKVYIIDEVHMLSIGAFNALLKTLEEPPEHCIFILATTEPHKIPLTIISRCQRFDFKRITSQAIVGRMNKIVDAEQLQVEEGSLEIIASAADGGMRDALSLLDQAISFSGDILKVEDALLITGAVSQLYIGKLAKSLHDKNVSDALETLNELLQQGKDPAKLIEDMIFYFRDMLLYKTAPGLEGVLEKVKVDETFRELSEQIPAQALYEMIDILNKSHQEMKWTNHPRIFFEVAVVKICQTSHQSAADLPEVDMLMKKIQQLEQEVERLKTTGIKAAAESPKKEAPRVPKGGKSNYKAPVGRIHEILKEATRPDLDLLRNSWGKLLAHLKQQNKVSHAALLNDSEPVAAGSAAFVLKFKYEIHCKMVAEDNNGVRTNLEQILESMLGKRMDLIGVPEAQWGKIREEFLEDHQQENEGSNEPAEEDPLIAEAKKLVGADLIEIKD"
                     /label="dnaX"
     primer_bind     complement(1802..1818)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1885..2473)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2664..3524)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3525..3629)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG TCCTACCAGG CGCTGTACCG CGTTTTCCGC CCGCAGAGAT TCGAGGACGT
       61 GGTTGGTCAG GAACATATCA CGAAAACGCT GCAAAATGCC CTGCTGCAGA AGAAGTTTAG
      121 CCATGCCTAC TTGTTCTCCG GCCCGCGCGG CACTGGTAAG ACGAGCGCGG CTAAAATCTT
      181 TGCGAAGGCG GTTAATTGCG AGCACGCGCC GGTAGACGAG CCGTGCAATG AGTGCGCAGC
      241 CTGTAAAGGC ATTACCAATG GTAGCATTTC GGATGTGATT GAAATCGACG CTGCGAGCAA
      301 CAACGGCGTT GATGAAATTC GTGATATCCG TGACAAAGTG AAGTTCGCAC CAAGCGCGGT
      361 CACCTACAAA GTGTACATTA TTGACGAGGT GCACATGCTG AGCATCGGTG CGTTCAATGC
      421 GCTGCTCAAG ACCCTGGAGG AACCGCCGGA GCACTGTATC TTCATTCTGG CCACCACGGA
      481 GCCGCACAAG ATTCCGCTGA CGATTATTTC GCGCTGTCAG CGTTTCGATT TCAAACGTAT
      541 TACCAGCCAG GCTATTGTGG GTCGCATGAA CAAAATCGTC GATGCCGAGC AGTTACAGGT
      601 TGAGGAAGGC AGCCTCGAAA TTATTGCGTC CGCCGCGGAT GGCGGCATGC GCGACGCACT
      661 GTCGTTACTG GATCAAGCCA TCAGCTTTTC TGGTGATATC CTGAAGGTGG AGGACGCGCT
      721 GCTCATCACC GGCGCCGTGA GCCAGCTGTA CATTGGTAAA CTGGCTAAGA GCTTGCATGA
      781 CAAAAACGTG TCAGATGCGT TGGAGACTTT AAATGAGCTG CTGCAACAAG GTAAGGACCC
      841 GGCAAAGCTG ATTGAGGACA TGATTTTCTA TTTTCGTGAC ATGCTGCTGT ATAAAACCGC
      901 GCCGGGCCTG GAAGGTGTCC TGGAAAAAGT TAAGGTTGAC GAAACGTTCC GTGAGCTGTC
      961 TGAGCAAATC CCGGCACAGG CCCTGTACGA AATGATCGAC ATTCTGAATA AAAGCCACCA
     1021 GGAAATGAAG TGGACCAACC ATCCGCGTAT TTTCTTCGAG GTGGCAGTCG TTAAAATCTG
     1081 TCAGACCTCC CACCAAAGCG CGGCGGACCT TCCGGAGGTG GACATGCTCA TGAAGAAAAT
     1141 CCAGCAATTG GAACAGGAGG TGGAACGCTT AAAGACCACC GGTATTAAAG CGGCAGCCGA
     1201 AAGCCCGAAA AAAGAAGCGC CGCGTGTTCC GAAAGGTGGC AAATCCAACT ACAAGGCGCC
     1261 GGTTGGTCGC ATTCACGAGA TCCTGAAAGA GGCAACCCGT CCGGATCTCG ACCTGTTGCG
     1321 TAACTCTTGG GGTAAACTGC TGGCACATCT GAAGCAGCAA AACAAAGTCA GCCACGCTGC
     1381 TCTGCTGAAT GACAGCGAAC CGGTGGCCGC GGGTTCGGCA GCGTTTGTGC TGAAGTTTAA
     1441 GTACGAAATT CACTGTAAGA TGGTCGCAGA GGACAACAAT GGTGTCCGCA CCAATCTGGA
     1501 ACAGATTCTG GAAAGCATGT TAGGTAAACG TATGGATCTG ATCGGCGTGC CGGAAGCGCA
     1561 ATGGGGCAAA ATTCGTGAAG AGTTTCTGGA GGACCATCAG CAGGAAAATG AAGGCAGCAA
     1621 TGAGCCGGCG GAGGAGGACC CGCTGATTGC AGAGGCGAAG AAACTGGTGG GCGCAGACCT
     1681 GATCGAAATC AAGGATTGAA GAGCTTAGAG ACCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1741 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1801 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1861 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1921 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1981 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     2041 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     2101 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     2161 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     2221 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2281 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2341 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2401 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2461 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2521 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2581 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2641 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2701 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2761 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2821 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2881 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2941 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     3001 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3061 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3121 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3181 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3241 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3301 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3361 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3421 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3481 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3541 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3601 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3661 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//