LOCUS       Exported     4018 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4018
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4004
                     /label="metS"
                     /note="Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation."
                     /translation="MPQENNTFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRLKGFDVRYLTGTDEHGQKIQQKAEQENITPQEYVDRAAADIQKLWKQLEISNDDFIRTTEKRHKVVIEKVFQKLLDNGDIYLDEYEGWYSIPDETFYTETQLVDIERNEKGEVIGGKSPDSGHPVELIKEESYFFRMGKYADRLLKYYEENPTFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGVKVPENPKHVVYVWIDALFNYLTALGYDTENDELYQKYWPADVHLVGKEIVRFHTIYWPIMLMALDLPLPKQVFAHGWLLMKDGKMSKSKGNVVDPVTLIERYGLDELRYYLLREVPFGSDGVFTPEGFVERINYDLANDLGNLLNRTVAMINKYFDGQIGSYKGAVTEFDHTLTSVAEETVKAYEKAMENMEFSVALSTLWQLISRTNKYIDETAPWVLAKDPAKEEELRSVMYHLAESLRISAVLLQPFLTKTPEKMFEQLGITDESLKAWDSITAFGQLKDTKVQKGEPLFPRLEAEEEIAYIKGKMQGSAPAKEETKEEEPQEVDRLPEITIDQFMDVELRVAEVIEAEPVKKADRLLKLQLDLGFEKRQVVSGIAKHYTPEELVGKKLVCVTNLKPVKLRGELSQGMILAGEADGVLKVVSIDQSLPKGTRIK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCCGCAAGA GAATAATACC TTCTATATTA
     2041 CCACCCCGAT TTACTATCCA AGCGGCAAAT TGCACATCGG TCACGCCTAC ACCACCGTCG
     2101 CAGGCGACGC CATGGCGCGC TATAAACGCC TGAAGGGCTT TGACGTGCGT TATCTGACGG
     2161 GCACCGATGA GCACGGTCAG AAAATTCAGC AAAAAGCTGA ACAGGAGAAC ATTACCCCGC
     2221 AAGAGTATGT GGACCGTGCG GCGGCTGATA TCCAGAAGTT GTGGAAACAA TTGGAGATTA
     2281 GCAACGATGA CTTTATCCGT ACCACGGAAA AACGTCACAA AGTCGTGATC GAGAAAGTTT
     2341 TTCAAAAGTT GCTGGATAAC GGTGATATCT ACCTGGACGA ATATGAGGGC TGGTACAGCA
     2401 TTCCGGACGA AACGTTTTAT ACGGAAACCC AGCTGGTTGA TATCGAACGC AACGAAAAGG
     2461 GTGAAGTTAT CGGTGGCAAA AGCCCGGATT CTGGTCACCC GGTTGAGCTG ATCAAGGAGG
     2521 AAAGCTATTT CTTTCGCATG GGTAAATACG CCGACCGCCT GCTGAAATAT TATGAAGAAA
     2581 ATCCTACCTT CATTCAACCT GAATCCCGTA AAAATGAAAT GATCAATAAT TTTATCAAGC
     2641 CGGGCCTGGA GGATCTGGCA GTTTCTCGTA CGACCTTCGA TTGGGGTGTC AAAGTGCCGG
     2701 AGAATCCGAA ACACGTGGTT TACGTTTGGA TCGATGCGCT GTTTAACTAT CTGACGGCGC
     2761 TGGGTTACGA CACGGAGAAT GACGAGCTGT ACCAAAAATA TTGGCCGGCG GACGTCCACC
     2821 TGGTTGGTAA GGAGATCGTT CGTTTTCACA CCATTTACTG GCCGATCATG CTGATGGCAC
     2881 TGGATCTGCC GTTGCCGAAA CAGGTTTTTG CACACGGCTG GCTGCTGATG AAAGACGGCA
     2941 AGATGAGCAA AAGCAAGGGC AACGTGGTCG ACCCGGTTAC TCTGATCGAG CGTTATGGCT
     3001 TAGATGAATT GCGTTACTAT CTGCTGCGTG AAGTCCCGTT CGGCTCCGAT GGCGTCTTTA
     3061 CTCCAGAAGG TTTCGTTGAA CGTATCAACT ACGATCTGGC TAACGATCTG GGCAATTTGT
     3121 TGAACCGCAC TGTGGCAATG ATTAATAAGT ACTTTGACGG TCAGATTGGC AGCTACAAAG
     3181 GCGCGGTGAC GGAGTTCGAC CACACTTTGA CGAGCGTGGC GGAGGAAACC GTCAAAGCAT
     3241 ACGAAAAGGC CATGGAAAAC ATGGAATTTA GCGTAGCGCT GAGCACCTTG TGGCAATTGA
     3301 TTAGCCGCAC GAACAAATAT ATTGATGAAA CGGCACCTTG GGTTTTGGCG AAAGACCCGG
     3361 CGAAGGAGGA GGAGCTGCGC TCCGTTATGT ACCATCTGGC GGAGTCCTTG CGCATTAGCG
     3421 CAGTTCTGCT CCAGCCGTTC CTGACCAAAA CTCCGGAGAA GATGTTTGAA CAATTGGGTA
     3481 TCACCGATGA GAGCCTGAAG GCCTGGGATA GCATCACCGC ATTCGGCCAA CTGAAGGACA
     3541 CGAAAGTTCA GAAGGGCGAG CCGCTGTTCC CGCGTCTGGA AGCGGAAGAG GAGATTGCCT
     3601 ATATCAAAGG TAAAATGCAA GGCAGCGCGC CTGCGAAGGA AGAAACCAAG GAAGAGGAAC
     3661 CGCAAGAAGT GGATCGCCTC CCGGAGATTA CGATCGATCA GTTTATGGAC GTTGAGCTGC
     3721 GTGTCGCGGA AGTTATCGAG GCCGAACCGG TTAAAAAAGC AGACCGCCTG CTGAAGCTGC
     3781 AACTGGACTT GGGTTTCGAA AAGCGCCAGG TTGTGAGCGG TATTGCGAAA CACTATACCC
     3841 CGGAGGAGCT GGTCGGTAAG AAGTTGGTCT GCGTTACCAA TCTGAAACCG GTAAAACTGC
     3901 GTGGTGAGCT GAGCCAAGGT ATGATCCTGG CTGGTGAGGC GGACGGTGTC CTGAAGGTTG
     3961 TGTCCATTGA TCAGTCACTC CCGAAGGGCA CCCGTATTAA GTGAAGAGCT TAGAGACC
//