LOCUS Exported 2974 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2974 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 1012..1059 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2907..2923 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2924..2955 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..2973 /label="prs" /note="Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P)." /translation="MSNQYGDKNLKIFSLNSNPELAKEIADIVGVQLGKCSVTRFSDGEVQINIEESIRGCDCYIIQSTSDPVNEHIMELLIMVDALKRASAKTINIVIPYYGYARQDRKARSREPITAKLFANLLETAGATRVIALDLHAPQIQGFFDIPIDHLMGVPILGEYFEGKNLEDIVIVSPDHGGVTRARKLADRLKAPIAIIDKRRPRPNVAEVMNIVGNIEGKTAILIDDIIDTAGTITLAANALVENGAKEVYACCTHPVLSGPAVERINNSTIKELVVTNSIKLPEEKKIERFKQLSVGPLLAEAIIRVHEQQSVSYLFS" primer_bind complement(1064..1080) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1147..1735) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1926..2786) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2787..2891) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 GTCTCAAATG AGCAATCAGT ATGGCGATAA AAATCTGAAG ATTTTCAGCC TGAACTCGAA 61 TCCGGAACTG GCAAAAGAGA TTGCCGATAT CGTTGGTGTC CAACTGGGTA AGTGTAGCGT 121 CACGAGATTT TCGGATGGTG AAGTCCAAAT CAATATTGAA GAAAGCATTC GTGGTTGCGA 181 TTGCTACATC ATCCAGAGCA CCAGCGACCC GGTTAACGAG CATATTATGG AGCTGCTGAT 241 CATGGTTGAT GCTCTGAAAC GTGCATCAGC CAAGACGATT AACATTGTTA TTCCGTACTA 301 CGGCTACGCA CGTCAAGATC GCAAGGCCCG TAGCCGTGAG CCAATTACGG CTAAACTGTT 361 TGCGAACTTG CTGGAAACCG CCGGCGCAAC CCGTGTGATC GCACTGGATC TGCACGCGCC 421 GCAAATCCAA GGATTTTTTG ATATCCCGAT TGACCACCTG ATGGGCGTTC CAATTCTGGG 481 TGAATACTTT GAGGGTAAGA ATCTGGAGGA CATTGTTATT GTGTCCCCGG ATCACGGCGG 541 CGTCACGCGT GCGCGTAAGC TGGCAGATCG TCTGAAGGCT CCGATTGCGA TTATTGACAA 601 GCGTCGTCCA CGTCCGAATG TTGCCGAAGT GATGAACATC GTTGGTAACA TCGAGGGCAA 661 AACTGCAATT TTGATTGACG ATATTATTGA CACCGCGGGT ACCATCACTC TGGCGGCAAA 721 TGCACTGGTT GAGAACGGTG CAAAGGAGGT TTATGCCTGC TGCACGCACC CGGTTCTGTC 781 TGGTCCGGCT GTGGAGCGTA TTAATAATAG CACGATCAAA GAGCTGGTTG TCACCAACTC 841 GATTAAACTG CCGGAGGAGA AAAAGATCGA GCGCTTCAAG CAGCTCAGCG TTGGTCCGCT 901 GCTGGCTGAA GCTATCATTC GCGTCCACGA GCAACAGTCC GTGTCCTACC TGTTCTCCTG 961 AAGAGCTTAG AGACCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA 1021 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG 1081 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC 1141 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 1201 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 1261 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 1321 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 1381 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 1441 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 1501 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 1561 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 1621 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 1681 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG 1741 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 1801 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 1861 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 1921 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 1981 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 2041 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA 2101 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 2161 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 2221 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 2281 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2341 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2401 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2461 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2521 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 2581 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 2641 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 2701 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 2761 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 2821 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 2881 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC 2941 CGGAGGCTTT TGACTTGGTC AGGTGGAGTG GGAG //