LOCUS       Exported     3442 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3442
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3428
                     /label="tilS"
                     /note="Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine."
                     /translation="MKSVKDFLNKHNLTLKGATIIVGVSGGPDSMALLHALHTLCGRSANVIAAHVDHRFRGAESEEDMRFVQAYCKAEQLVCETAQINVTAYAQEKGLNKQAAARDCRYQFFEEIMSKHQADYLALAHHGDDQVETMLMKLAKGTLGTGLAGMQPVRRFGTGRIIRPFLTITKEEILHYCHENGLSYRTDESNAKDDYTRNRFRKTVLPFLKQESPDVHKRFQKVSEALTEDEQFLQSLTKDEMNKVITSQSNTSVEINSSQLLALPMPLQRRGVQLILNYLYENVPSSFSAHHIQQFLDWAENGGPSGVLDFPKGLKVVKSYQTCLFTFEQWQCKNVPFEYQISGAADETAVLPNGYLIEARHYADSPEEHGNAVFITSEKKVRFPLTIRTRKAGDRIKLKGMNGSKKVKDIFIDKKLPLQERDNWPIVTDASGEIIWIPGLKKSIFEDLVIPNSDRIVLQYRQHEKCRGQAKS"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAATCCGT GAAAGACTTC TTGAACAAAC
     2041 ACAACCTGAC CCTGAAAGGC GCCACTATCA TCGTGGGTGT CTCAGGTGGT CCGGATAGCA
     2101 TGGCGCTGTT GCACGCCCTG CATACCCTGT GCGGTCGTTC TGCGAACGTG ATTGCGGCCC
     2161 ATGTGGACCA TCGTTTCCGT GGCGCAGAAT CTGAGGAGGA TATGCGTTTT GTTCAGGCAT
     2221 ACTGTAAGGC AGAGCAGTTG GTTTGCGAAA CCGCGCAAAT TAACGTCACG GCGTACGCGC
     2281 AAGAAAAAGG TCTGAATAAG CAAGCTGCAG CTCGTGATTG TCGTTACCAG TTTTTTGAAG
     2341 AGATTATGAG CAAGCACCAG GCGGACTACC TGGCGCTGGC GCACCACGGT GATGACCAAG
     2401 TAGAAACCAT GTTGATGAAG CTGGCGAAGG GTACCCTCGG TACCGGCCTG GCGGGTATGC
     2461 AACCGGTGCG CCGTTTTGGT ACGGGTCGTA TTATCCGTCC GTTCTTGACC ATTACCAAGG
     2521 AGGAAATTCT CCACTACTGC CATGAGAATG GTCTGAGCTA CCGTACCGAC GAATCCAACG
     2581 CAAAGGACGA CTATACGCGC AATCGTTTTC GCAAGACCGT CTTGCCGTTC CTGAAGCAAG
     2641 AAAGCCCGGA CGTCCACAAG CGTTTTCAGA AAGTTAGCGA AGCGCTGACC GAGGACGAGC
     2701 AGTTCCTGCA GAGCTTGACC AAAGACGAAA TGAACAAGGT GATCACCAGC CAATCCAACA
     2761 CCAGCGTGGA GATCAACAGC TCTCAGCTGT TGGCGCTGCC GATGCCGCTG CAACGTCGTG
     2821 GCGTCCAACT GATCCTGAAT TACCTTTACG AGAATGTCCC AAGCTCCTTC TCCGCACACC
     2881 ATATCCAGCA ATTCCTGGAT TGGGCTGAAA ATGGCGGTCC GAGCGGTGTA CTGGATTTCC
     2941 CGAAGGGTCT GAAGGTGGTT AAAAGCTATC AGACCTGTCT GTTTACCTTC GAACAGTGGC
     3001 AATGCAAAAA TGTGCCTTTC GAATACCAAA TTTCTGGTGC GGCGGACGAA ACGGCAGTTC
     3061 TGCCGAATGG TTACCTGATC GAAGCGCGTC ATTATGCGGA TAGCCCGGAG GAGCACGGTA
     3121 ACGCGGTTTT CATTACGAGC GAGAAGAAAG TCCGTTTTCC ACTGACCATC CGTACGCGCA
     3181 AGGCGGGTGA CCGCATCAAA CTGAAGGGTA TGAATGGTTC GAAGAAAGTG AAAGACATTT
     3241 TCATCGATAA AAAGCTGCCT CTGCAGGAGC GTGATAATTG GCCGATTGTG ACGGACGCAA
     3301 GCGGTGAGAT TATTTGGATT CCGGGTCTGA AGAAAAGCAT TTTTGAAGAT CTGGTGATTC
     3361 CTAACAGCGA CCGTATTGTC CTGCAGTACC GCCAGCACGA AAAATGTCGC GGTCAAGCCA
     3421 AGTCCTGAAG AGCTTAGAGA CC
//