LOCUS       Exported     3421 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3421
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1459..1506
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3354..3370
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3371..3402
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3420
                     /translation="MTITLYNTLTRQKETFVPLEEGKVKMYVCGPTVYNYIHIGNARPAIVYDTVRNYLEYKGYDVQYVSNFTDVDDKLIKAANELGEDVPTISERFIKAYFEDVGALGCRKADLHPRVMENMDAIIEFVDQLVKKGYAYESEGDVYFKTRAFEGYGKLSQQSIDELRSGARIRVGEKKEDALDFALWKAAKEGEISWDSPWGKGRPGWHIECSAMVKKYLGDQIDIHAGGQDLTFPHHENEIAQSEALTGKTFAKYWLHNGYINIDNEKMSKSLGNFVLVHDIIKQHDPQLLRFFMLSVHYRHPINYSEELLENTKSAFSRLKTAYSNLQHRLNSSTNLTEDDDQWLEKVEEHRKAFEEEMDDDFNTANAISVLFDLAKHANYYLQKDHTADHVITAFIEMFDRIVSVLGFSLGEQELLDQEIEDLIEKRNEARRNRDFALSDQIRDQLKSMNIILEDTAQGTRWKRGE"
                     /label="cysS"
                     /note=""
     primer_bind     complement(1511..1527)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1594..2182)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2373..3233)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3234..3338)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 GTCTCAAATG ACCATTACCC TGTATAACAC CCTGACCCGT CAAAAAGAAA CCTTTGTGCC
       61 GCTGGAGGAG GGTAAGGTGA AGATGTACGT CTGCGGTCCG ACGGTTTATA ATTACATTCA
      121 TATCGGCAAT GCGCGTCCAG CGATCGTGTA TGATACCGTC CGTAATTATC TGGAGTACAA
      181 GGGTTATGAT GTTCAATATG TCTCGAACTT CACGGATGTC GACGATAAGC TGATCAAGGC
      241 CGCAAATGAG CTGGGCGAAG ATGTGCCGAC GATCTCCGAA CGTTTTATCA AAGCATACTT
      301 CGAAGATGTT GGTGCCCTGG GTTGCCGTAA AGCGGATCTG CACCCGCGTG TTATGGAAAA
      361 CATGGACGCT ATCATTGAAT TCGTCGACCA GCTGGTCAAA AAGGGCTATG CCTACGAGAG
      421 CGAGGGTGAC GTTTACTTCA AGACCCGCGC TTTTGAAGGT TACGGTAAGC TGAGCCAACA
      481 GTCCATCGAT GAGCTGCGCT CTGGTGCACG TATCCGCGTC GGCGAGAAAA AAGAGGATGC
      541 GCTGGACTTT GCCCTGTGGA AAGCCGCGAA AGAAGGCGAG ATCTCTTGGG ACAGCCCGTG
      601 GGGTAAAGGC CGTCCGGGCT GGCACATTGA ATGCAGCGCA ATGGTCAAGA AATATCTGGG
      661 TGATCAGATC GACATCCATG CGGGCGGTCA AGATCTGACT TTTCCTCACC ATGAGAACGA
      721 AATCGCGCAG AGCGAGGCGC TGACTGGCAA AACCTTTGCC AAGTACTGGC TGCATAACGG
      781 TTACATTAAT ATCGACAACG AAAAAATGTC TAAGTCACTG GGCAACTTCG TCCTGGTGCA
      841 CGATATCATT AAGCAGCATG ACCCGCAACT CCTGCGCTTT TTTATGTTGT CCGTTCACTA
      901 TCGCCACCCG ATCAATTATA GCGAGGAACT GCTTGAGAAC ACCAAAAGCG CATTTAGCCG
      961 TTTGAAAACC GCGTACAGCA ACCTCCAACA CCGTTTAAAC AGCTCTACCA ACCTTACCGA
     1021 GGACGATGAT CAGTGGCTGG AAAAAGTTGA GGAGCACCGC AAGGCGTTTG AGGAAGAAAT
     1081 GGATGACGAC TTTAATACCG CCAATGCCAT CTCTGTCCTG TTCGACCTGG CGAAACATGC
     1141 AAATTATTAC TTGCAAAAAG ATCATACCGC GGATCACGTT ATTACCGCCT TCATTGAAAT
     1201 GTTCGATCGC ATTGTGTCCG TCCTGGGTTT CAGCCTGGGT GAACAAGAGC TGTTGGATCA
     1261 GGAGATCGAG GATCTGATCG AGAAACGTAA TGAAGCACGT CGCAACCGCG ACTTCGCACT
     1321 GAGCGACCAG ATCCGTGATC AGCTGAAAAG CATGAATATT ATCTTAGAAG ATACGGCACA
     1381 GGGTACGCGT TGGAAGAGAG GCGAGTGAAG AGCTTAGAGA CCGCTGAGGT GTCAATCGTC
     1441 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1501 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1561 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2161 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2221 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2281 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2341 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2401 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2461 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2521 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2581 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     2641 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2701 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2761 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2821 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2881 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2941 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3001 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3061 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3121 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3181 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3241 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3301 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3361 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     3421 G
//