LOCUS Exported 3421 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3421 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1459..1506 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3354..3370 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3371..3402 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3420 /translation="MTITLYNTLTRQKETFVPLEEGKVKMYVCGPTVYNYIHIGNARPAIVYDTVRNYLEYKGYDVQYVSNFTDVDDKLIKAANELGEDVPTISERFIKAYFEDVGALGCRKADLHPRVMENMDAIIEFVDQLVKKGYAYESEGDVYFKTRAFEGYGKLSQQSIDELRSGARIRVGEKKEDALDFALWKAAKEGEISWDSPWGKGRPGWHIECSAMVKKYLGDQIDIHAGGQDLTFPHHENEIAQSEALTGKTFAKYWLHNGYINIDNEKMSKSLGNFVLVHDIIKQHDPQLLRFFMLSVHYRHPINYSEELLENTKSAFSRLKTAYSNLQHRLNSSTNLTEDDDQWLEKVEEHRKAFEEEMDDDFNTANAISVLFDLAKHANYYLQKDHTADHVITAFIEMFDRIVSVLGFSLGEQELLDQEIEDLIEKRNEARRNRDFALSDQIRDQLKSMNIILEDTAQGTRWKRGE" /label="cysS" /note="" primer_bind complement(1511..1527) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1594..2182) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2373..3233) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3234..3338) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 GTCTCAAATG ACCATTACCC TGTATAACAC CCTGACCCGT CAAAAAGAAA CCTTTGTGCC 61 GCTGGAGGAG GGTAAGGTGA AGATGTACGT CTGCGGTCCG ACGGTTTATA ATTACATTCA 121 TATCGGCAAT GCGCGTCCAG CGATCGTGTA TGATACCGTC CGTAATTATC TGGAGTACAA 181 GGGTTATGAT GTTCAATATG TCTCGAACTT CACGGATGTC GACGATAAGC TGATCAAGGC 241 CGCAAATGAG CTGGGCGAAG ATGTGCCGAC GATCTCCGAA CGTTTTATCA AAGCATACTT 301 CGAAGATGTT GGTGCCCTGG GTTGCCGTAA AGCGGATCTG CACCCGCGTG TTATGGAAAA 361 CATGGACGCT ATCATTGAAT TCGTCGACCA GCTGGTCAAA AAGGGCTATG CCTACGAGAG 421 CGAGGGTGAC GTTTACTTCA AGACCCGCGC TTTTGAAGGT TACGGTAAGC TGAGCCAACA 481 GTCCATCGAT GAGCTGCGCT CTGGTGCACG TATCCGCGTC GGCGAGAAAA AAGAGGATGC 541 GCTGGACTTT GCCCTGTGGA AAGCCGCGAA AGAAGGCGAG ATCTCTTGGG ACAGCCCGTG 601 GGGTAAAGGC CGTCCGGGCT GGCACATTGA ATGCAGCGCA ATGGTCAAGA AATATCTGGG 661 TGATCAGATC GACATCCATG CGGGCGGTCA AGATCTGACT TTTCCTCACC ATGAGAACGA 721 AATCGCGCAG AGCGAGGCGC TGACTGGCAA AACCTTTGCC AAGTACTGGC TGCATAACGG 781 TTACATTAAT ATCGACAACG AAAAAATGTC TAAGTCACTG GGCAACTTCG TCCTGGTGCA 841 CGATATCATT AAGCAGCATG ACCCGCAACT CCTGCGCTTT TTTATGTTGT CCGTTCACTA 901 TCGCCACCCG ATCAATTATA GCGAGGAACT GCTTGAGAAC ACCAAAAGCG CATTTAGCCG 961 TTTGAAAACC GCGTACAGCA ACCTCCAACA CCGTTTAAAC AGCTCTACCA ACCTTACCGA 1021 GGACGATGAT CAGTGGCTGG AAAAAGTTGA GGAGCACCGC AAGGCGTTTG AGGAAGAAAT 1081 GGATGACGAC TTTAATACCG CCAATGCCAT CTCTGTCCTG TTCGACCTGG CGAAACATGC 1141 AAATTATTAC TTGCAAAAAG ATCATACCGC GGATCACGTT ATTACCGCCT TCATTGAAAT 1201 GTTCGATCGC ATTGTGTCCG TCCTGGGTTT CAGCCTGGGT GAACAAGAGC TGTTGGATCA 1261 GGAGATCGAG GATCTGATCG AGAAACGTAA TGAAGCACGT CGCAACCGCG ACTTCGCACT 1321 GAGCGACCAG ATCCGTGATC AGCTGAAAAG CATGAATATT ATCTTAGAAG ATACGGCACA 1381 GGGTACGCGT TGGAAGAGAG GCGAGTGAAG AGCTTAGAGA CCGCTGAGGT GTCAATCGTC 1441 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT 1501 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA 1561 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA 1621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 1681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 1801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 1861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 1981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 2041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 2101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 2161 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT 2221 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 2281 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 2341 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 2401 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 2461 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA 2521 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 2581 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 2641 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 2701 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 2761 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 2821 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 2881 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 2941 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 3001 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 3061 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 3121 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 3181 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 3241 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 3301 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC 3361 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA 3421 G //