LOCUS       Exported     4102 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4102
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4088
                     /label="fusA"
                     /note="Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity)."
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILFYTGRIHKIGETHEGASQMDWMEQEQERGITITSAATTAQWKGYRVNIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKIGADFLYSVGTLRDRLQANAHAIQLPIGAEDNFEGIIDLVENVAYFYEDDLGTRSDAKEIPEEYKEQAEELRNSLIEAVCELDEELMDKYLEGEEITIDELKAGIRKGTLNVEFYPVLVGSAFKNKGVQLVLDAVLDYLPAPTDVAAIKGTRPDTNEEIERHSSDEEPFSALAFKVMTDPYVGKLTFFRVYSGTLDSGSYVKNSTKGKRERVGRILQMHANSREEISTVYAGDIAAAVGLKDTTTGDTLCDEKDLVILESMEFPEPVIDVAIEPKSKADQDKMGIALAKLAEEDPTFRTQTNPETGQTIISGMGELHLDIIVDRMKREFKVEANVGAPQVAYRETFRTGAKVEGKFVRQSGGRGQFGHVWIEFEPNEEGAGFEFENAIVGGVVPREYIPAVQAGLEDALENGVLAGFPLIDIKAKLFDGSYHDVDSNEMAFKVAASMALKNAVSKCNPVLLEPIMKVEVVIPEEYMGDIMGDITSRRGRVEGMEARGNAQVVRAMVPLAEMFGYATALRSNTQGRGTFTMHMDHYEEVPKSVAEEIIKKNKGE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGCACGTGA ATTTAGCCTG GAAAAGACGC
     2041 GTAACATTGG CATTATGGCA CATATCGACG CCGGTAAAAC CACGACTACG GAGCGCATTC
     2101 TGTTCTACAC TGGCCGCATC CACAAAATTG GCGAAACCCA CGAGGGTGCT AGCCAAATGG
     2161 ACTGGATGGA ACAAGAGCAG GAGCGTGGTA TCACCATTAC TAGCGCGGCA ACTACGGCCC
     2221 AATGGAAAGG TTATAGAGTC AACATTATTG ATACCCCGGG CCATGTGGAC TTCACCGTGG
     2281 AGGTCGAGCG TAGCTTACGT GTTTTGGATG GTGCGGTCGC AGTGCTGGAC GCGCAGAGCG
     2341 GTGTTGAGCC GCAAACCGAA ACCGTGTGGC GTCAAGCTAC GACCTACGGT GTCCCGCGTA
     2401 TCGTTTTTGT CAATAAAATG GATAAAATTG GTGCGGACTT CCTGTATAGC GTTGGCACCC
     2461 TGCGTGATCG TCTGCAAGCA AACGCACACG CAATTCAGCT GCCGATTGGT GCGGAGGACA
     2521 ACTTCGAAGG TATCATCGAC CTGGTCGAAA ACGTCGCGTA TTTCTACGAA GATGACCTGG
     2581 GTACGCGCTC GGATGCTAAA GAGATCCCGG AAGAGTACAA GGAACAGGCG GAGGAACTGC
     2641 GCAACAGCTT GATCGAGGCA GTGTGTGAGC TGGACGAGGA GCTGATGGAC AAATATCTGG
     2701 AAGGTGAAGA AATCACCATC GACGAACTCA AAGCTGGTAT TCGCAAGGGC ACCTTGAACG
     2761 TGGAGTTTTA TCCAGTTCTG GTCGGCAGCG CGTTTAAAAA TAAGGGTGTT CAACTGGTAC
     2821 TGGATGCCGT CCTGGACTAT CTGCCGGCGC CGACGGATGT GGCCGCAATC AAGGGTACGC
     2881 GCCCAGACAC CAACGAGGAA ATTGAGCGTC ACAGCTCGGA TGAGGAGCCG TTTAGCGCAT
     2941 TAGCGTTTAA AGTTATGACC GACCCGTACG TTGGTAAACT GACCTTCTTC CGTGTCTACA
     3001 GCGGCACGCT GGACTCTGGC AGCTATGTGA AGAACAGCAC CAAAGGTAAA CGTGAGCGCG
     3061 TCGGTCGTAT TCTGCAAATG CACGCAAATA GCCGTGAGGA GATTAGCACC GTGTACGCTG
     3121 GTGATATCGC CGCAGCGGTA GGCCTGAAAG ACACCACGAC CGGCGATACG CTGTGCGACG
     3181 AGAAGGACCT GGTTATTCTG GAAAGCATGG AGTTCCCGGA ACCTGTGATC GACGTTGCGA
     3241 TTGAGCCTAA GAGCAAAGCG GACCAGGACA AAATGGGAAT CGCGTTAGCA AAGCTGGCGG
     3301 AAGAGGACCC GACCTTCCGC ACGCAGACGA ACCCGGAAAC CGGTCAGACG ATCATTTCCG
     3361 GTATGGGTGA GCTGCACCTG GACATTATCG TTGACCGTAT GAAACGCGAA TTTAAAGTGG
     3421 AAGCAAACGT GGGTGCCCCG CAAGTTGCGT ACCGCGAAAC GTTTCGTACC GGTGCGAAAG
     3481 TGGAAGGTAA ATTCGTGCGT CAGTCGGGTG GCCGCGGTCA ATTCGGTCAT GTTTGGATCG
     3541 AGTTCGAGCC GAATGAAGAG GGTGCTGGTT TTGAGTTTGA GAATGCAATC GTGGGCGGCG
     3601 TGGTGCCGCG TGAATATATC CCTGCCGTTC AGGCAGGCCT GGAGGACGCA TTGGAGAACG
     3661 GTGTTCTGGC TGGTTTTCCA CTGATCGATA TCAAGGCTAA GCTGTTCGAT GGTAGCTACC
     3721 ATGATGTCGA TAGCAATGAG ATGGCATTCA AGGTTGCAGC GTCCATGGCG CTCAAAAATG
     3781 CGGTGAGCAA ATGCAACCCG GTGCTGCTGG AACCGATCAT GAAGGTTGAG GTGGTCATTC
     3841 CTGAGGAATA TATGGGTGAT ATCATGGGTG ATATCACGTC CCGTCGCGGT CGTGTCGAAG
     3901 GCATGGAAGC GCGTGGCAAC GCGCAAGTCG TACGTGCAAT GGTTCCGTTG GCGGAGATGT
     3961 TTGGCTATGC AACGGCGCTC CGCAGCAACA CCCAGGGTCG TGGCACCTTC ACCATGCACA
     4021 TGGATCACTA CGAAGAAGTT CCCAAGAGCG TTGCAGAAGA GATTATTAAG AAAAACAAAG
     4081 GTGAGTGAAG AGCTTAGAGA CC
//