LOCUS       Exported     3211 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3211
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1249..1296
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3144..3160
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3161..3192
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3210
                     /note="This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis."
                     /translation="MAKEKFDRSKSHANIGTIGHVDHGKTTLTAAITTVLHKKSGKGTAMAYDQIDGAPEERERGITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSKNVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYDFPGDDVPVVKGSALKALEGDAEWEAKIFELMDAVDEYIPTPERDTEKPFMMPVEDVFSITGRGTVATGRVERGQVKVGDEVEIIGLQEENKKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREEIQRGQVLAKPGTITPHSKFKAEVYVLSKEEGGRHTPFFSNYRPQFYFRTTDVTGIIHLPEGVEMVMPGDNTEMNVELISTIAIEEGTRFSIREGGRTVGSGVVSTITE"
                     /label="tufA"
     primer_bind     complement(1301..1317)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1384..1972)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2163..3023)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3024..3128)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 GTCTCAAATG GCGAAGGAGA AATTTGACCG CTCCAAGTCC CACGCCAATA TCGGTACCAT
       61 CGGCCACGTT GATCATGGTA AAACGACGTT AACCGCCGCG ATTACCACCG TCCTCCACAA
      121 AAAGAGCGGC AAAGGTACTG CCATGGCATA TGATCAAATT GACGGTGCAC CGGAGGAGCG
      181 TGAGCGCGGT ATTACCATTA GCACCGCACA CGTGGAATAT GAAACTGAAA CCCGTCATTA
      241 CGCCCACGTG GACTGCCCGG GTCACGCTGA TTATGTTAAA AATATGATCA CCGGTGCGGC
      301 ACAGATGGAT GGTGCGATCC TGGTCGTTAG CGCAGCCGAT GGCCCTATGC CGCAAACGCG
      361 TGAGCATATT CTGCTGAGCA AGAACGTGGG CGTTCCTTAC ATTGTTGTTT TTCTGAACAA
      421 GTGCGACATG GTCGATGACG AGGAACTGCT GGAGCTCGTG GAGATGGAGG TGCGCGATCT
      481 GCTGAGCGAG TATGATTTCC CGGGTGATGA CGTTCCGGTT GTCAAAGGCA GCGCGCTGAA
      541 AGCGCTCGAG GGCGACGCCG AGTGGGAAGC GAAAATCTTC GAGCTGATGG ATGCGGTGGA
      601 CGAATATATC CCGACGCCGG AGCGCGATAC TGAGAAGCCG TTTATGATGC CGGTCGAGGA
      661 CGTCTTTAGC ATCACGGGTC GTGGTACGGT AGCGACGGGT CGTGTAGAGC GTGGTCAGGT
      721 CAAGGTTGGC GACGAGGTGG AAATCATCGG TCTGCAGGAA GAGAATAAAA AGACCACGGT
      781 GACCGGCGTC GAAATGTTCC GCAAGCTCCT GGATTACGCG GAGGCCGGCG ACAACATCGG
      841 TGCTCTGCTG CGTGGTGTTA GCCGTGAAGA AATTCAGCGC GGTCAGGTGC TGGCCAAGCC
      901 GGGTACGATT ACGCCGCACA GCAAGTTTAA AGCAGAAGTG TACGTCCTGT CCAAAGAAGA
      961 AGGTGGCCGT CATACCCCGT TCTTCTCTAA TTACCGCCCG CAATTCTACT TCCGTACCAC
     1021 CGACGTTACC GGCATTATTC ATCTGCCGGA GGGTGTGGAA ATGGTCATGC CGGGTGACAA
     1081 TACCGAGATG AACGTTGAAC TCATCTCCAC TATCGCGATC GAGGAAGGTA CCCGTTTCAG
     1141 CATTCGTGAA GGTGGCCGCA CCGTTGGCAG CGGTGTTGTG AGCACGATTA CCGAGTGAAG
     1201 AGCTTAGAGA CCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1261 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1321 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1381 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1441 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1501 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1561 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1621 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1681 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1741 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1801 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1861 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     1921 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     1981 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2041 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2101 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2161 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2221 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2281 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2341 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2401 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2461 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2521 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2581 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2641 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2701 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2761 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     2821 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     2881 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     2941 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     3001 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     3061 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     3121 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3181 AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA G
//