LOCUS       Exported     3826 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3826
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3812
                     /translation="MCGIVGYIGQLDAKEILLKGLEKLEYRGYDSAGIAVANEQGIHVFKEKGRIADLREVVDANVEAKAGIGHTRWATHGEPSYLNAHPHQSALGRFTLVHNGVIENYVQLKQEYLQDVELKSDTDTEVVVQVIEQFVNGGLETEEAFRKTLTLLKGSYAIALFDNDNRETIFVAKNKSPLLVGLGDTFNVVASDAMAMLQVTNEYVELMDKEMVIVTDDQVVIKNLDGDVITRASYIAELDASDIEKGTYPHYMLKETDEQPVVMRKIIQTYQDENGKLSVPGDIAAAVAEADRIYIIGCGTSYHAGLVGKQYIEMWANVPVEVHVASEFSYNMPLLSKKPLFIFLSQSGETADSRAVLVQVKALGHKALTITNVPGSTLSREADYTLLLHAGPEIAVASTKAYTAQIAVLAVLASVAADKNGINIGFDLVKELGIAANAMEALCDQKDEMEMIAREYLTVSRNAFFIGRGLDYFVCVEGALKLKEISYIQAEGFAGGELKHGTIALIEQGTPVFALATQEHVNLSIRGNVKEVAARGANTCIISLKGLDDADDRFVLPEVNPALAPLVSVVPLQLIAYYAALHRGCDVDKPRNLAKSVTVE"
                     /label="glmS"
                     /note="Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTGCGGTAT CGTTGGTTAT ATCGGTCAGC
     2041 TGGATGCGAA GGAGATTCTG CTGAAGGGTC TGGAAAAATT AGAATACCGT GGTTACGATA
     2101 GCGCTGGCAT TGCGGTGGCC AATGAGCAGG GTATTCACGT TTTTAAGGAG AAGGGCCGTA
     2161 TTGCGGATCT GCGCGAGGTG GTGGACGCAA ATGTGGAGGC AAAGGCGGGT ATTGGCCATA
     2221 CGCGTTGGGC GACCCATGGT GAGCCGAGCT ATCTGAATGC GCACCCGCAC CAGAGCGCGC
     2281 TGGGCCGTTT CACCCTGGTG CACAACGGTG TCATTGAAAA CTACGTTCAA TTAAAACAGG
     2341 AGTATCTGCA AGACGTGGAA CTGAAAAGCG ACACGGACAC CGAGGTGGTT GTGCAAGTGA
     2401 TCGAACAGTT CGTTAACGGT GGTCTGGAAA CGGAAGAAGC GTTTCGCAAA ACGCTGACGC
     2461 TGCTGAAGGG TAGCTACGCT ATCGCGTTGT TCGACAATGA TAATCGTGAA ACCATTTTCG
     2521 TTGCGAAGAA CAAGAGCCCG CTGCTGGTGG GCCTGGGTGA CACTTTCAAT GTTGTTGCGA
     2581 GTGATGCTAT GGCCATGCTG CAAGTTACCA ATGAATACGT GGAACTGATG GATAAGGAAA
     2641 TGGTCATTGT CACCGATGAC CAGGTGGTGA TTAAAAACCT GGACGGTGAT GTGATTACCC
     2701 GTGCAAGCTA TATCGCGGAG CTGGACGCCA GCGACATTGA GAAAGGCACG TACCCTCACT
     2761 ACATGTTGAA AGAAACCGAC GAGCAACCTG TTGTCATGCG TAAGATTATC CAGACCTACC
     2821 AGGACGAGAA CGGTAAGCTG TCTGTGCCGG GTGATATCGC AGCGGCTGTC GCGGAAGCGG
     2881 ATCGTATTTA CATCATTGGT TGCGGTACCA GCTACCACGC GGGTCTGGTT GGCAAGCAGT
     2941 ATATCGAGAT GTGGGCCAAC GTGCCGGTGG AAGTGCACGT GGCCAGCGAG TTCTCGTATA
     3001 ACATGCCGCT GCTGTCCAAG AAACCGTTGT TCATCTTTCT GAGCCAGAGC GGTGAAACCG
     3061 CAGACTCGCG TGCAGTGCTG GTTCAAGTGA AAGCTCTGGG TCACAAGGCT CTGACCATTA
     3121 CCAATGTGCC GGGCAGCACC CTGAGCCGTG AGGCAGATTA CACGCTGCTG CTGCACGCGG
     3181 GCCCGGAAAT TGCGGTTGCG AGCACCAAGG CGTATACGGC ACAGATCGCG GTGCTGGCAG
     3241 TTTTGGCTAG CGTCGCCGCG GACAAGAATG GCATCAACAT TGGTTTTGAC CTGGTCAAAG
     3301 AACTGGGTAT CGCGGCAAAT GCCATGGAAG CCTTGTGCGA TCAGAAAGAC GAGATGGAAA
     3361 TGATTGCGCG TGAGTATTTG ACGGTCAGCC GCAACGCCTT CTTCATCGGC CGTGGTCTGG
     3421 ACTACTTCGT GTGTGTCGAG GGCGCCCTGA AGCTGAAAGA GATTAGCTAC ATCCAAGCAG
     3481 AAGGTTTTGC GGGTGGTGAA CTGAAACACG GCACCATTGC CCTGATTGAG CAGGGCACTC
     3541 CGGTGTTCGC CCTGGCAACC CAAGAGCACG TCAATCTGTC TATCCGCGGT AACGTGAAAG
     3601 AAGTGGCGGC GCGTGGTGCT AACACATGCA TCATCAGCCT GAAAGGCTTG GACGACGCGG
     3661 ATGACCGCTT CGTGCTGCCA GAGGTGAACC CGGCCCTGGC GCCGCTGGTG AGCGTTGTCC
     3721 CTCTTCAACT GATTGCGTAC TATGCCGCCT TGCATCGTGG CTGTGACGTT GACAAGCCGC
     3781 GTAATCTGGC CAAAAGCGTT ACTGTCGAGT GAAGAGCTTA GAGACC
//