LOCUS Exported 3061 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3061 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1099..1146 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2994..3010 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3011..3042 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3060 /note="Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB; this reaction does not require ATP in vitro. TsaD likely plays a direct catalytic role in this reaction." /translation="MSEQKDMYVLGIETSCDETAAAIVKNGKEIISNVVASQIESHKRFGGVVPEIASRHHVEQITLVIEEAFRKAGMTYSDIDAIAVTEGPGLVGALLIGVNAAKALSFAYNIPLVGVHHIAGHIYANRLVEDIVFPALALVVSGGHTELVYMKEHGSFEVIGETLDDAAGEAYDKVARTMGLPYPGGPQIDKLAEKGNDNIPLPRAWLEEGSYNFSFSGLKSAVINTLHNASQKGQEIAPEDLSASFQNSVIDVLVTKTARAAKEYDVKQVLLAGGVAANRGLRAALEKEFAQHEGITLVIPPLALCTDNAAMIAAAGTIAFEKGIRGAYDMNGQPGLELTSYQSLTR" /label="gcp" primer_bind complement(1151..1167) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1234..1822) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2013..2873) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2874..2978) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 GTCTCAAATG TCCGAACAAA AGGATATGTA TGTCCTGGGT ATTGAAACCA GCTGTGACGA 61 AACCGCGGCG GCGATCGTGA AAAACGGCAA GGAGATTATC AGCAACGTTG TGGCCAGCCA 121 AATTGAGAGC CACAAACGTT TTGGCGGTGT TGTCCCGGAG ATCGCAAGCC GTCACCACGT 181 GGAGCAGATC ACCCTGGTCA TCGAGGAAGC GTTTCGTAAA GCGGGTATGA CCTACAGCGA 241 CATCGACGCT ATTGCGGTTA CGGAGGGCCC GGGTCTGGTC GGTGCCCTGC TGATTGGTGT 301 TAACGCAGCA AAGGCACTGA GCTTTGCGTA TAATATCCCA CTGGTCGGTG TTCACCATAT 361 CGCTGGCCAC ATTTACGCCA ATCGTCTGGT CGAGGATATC GTGTTCCCGG CACTGGCACT 421 GGTGGTTAGC GGTGGTCACA CGGAACTGGT CTATATGAAA GAACACGGTT CGTTTGAAGT 481 AATCGGTGAA ACGTTGGACG ATGCTGCTGG CGAAGCATAC GACAAGGTGG CACGTACGAT 541 GGGTCTGCCA TATCCGGGTG GCCCGCAAAT TGATAAATTA GCTGAGAAAG GCAATGATAA 601 TATCCCGCTC CCGCGTGCGT GGCTGGAGGA GGGTAGCTAC AACTTCAGCT TTTCTGGTTT 661 AAAGAGCGCG GTTATTAATA CCCTGCACAA TGCTTCCCAG AAAGGTCAGG AGATTGCGCC 721 GGAGGACTTA AGCGCCAGCT TTCAGAACAG CGTGATTGAC GTGCTGGTCA CCAAAACCGC 781 TCGTGCGGCG AAGGAGTACG ATGTCAAACA GGTTTTGCTG GCGGGCGGTG TCGCCGCTAA 841 CCGTGGTCTG CGTGCGGCGT TAGAAAAGGA GTTCGCGCAG CACGAGGGTA TTACCCTGGT 901 GATTCCGCCG CTGGCACTGT GTACGGATAA CGCGGCTATG ATCGCTGCGG CGGGTACTAT 961 TGCATTTGAG AAGGGTATCC GCGGCGCGTA CGACATGAAT GGTCAGCCGG GTTTGGAGCT 1021 GACGAGCTAT CAGAGCCTGA CCCGCTGAAG AGCTTAGAGA CCGCTGAGGT GTCAATCGTC 1081 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT 1141 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA 1201 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA 1261 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 1321 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1381 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 1441 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 1501 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1561 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 1621 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 1681 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 1741 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 1801 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT 1861 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 1921 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 1981 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 2041 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 2101 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA 2161 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 2221 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 2281 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 2341 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 2401 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 2461 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 2521 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 2581 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 2641 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 2701 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 2761 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 2821 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 2881 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 2941 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC 3001 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA 3061 G //