LOCUS       Exported     4030 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4030
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4016
                     /label="ligA"
                     /note="DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA."
                     /translation="MDKETAKQRAEELRRTINKYSYEYYTLDEPSVPDAEYDRLMQELIAIEEEHPDLRTPDSPTQRVGGAVLEAFQKVTHGTPMLSLGNAFNADDLRDFDRRVRQSVGDDVAYNVELKIDGLAVSLRYEDGYFVRGATRGDGTTGEDITENLKTIRNIPLKMNRELSIEVRGEAYMPKRSFEALNEERIKNEEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFVYSIAELDEMGVETQSQGLDFLDELGFKTNQERKKCGSIEEVITLIDELQAKRADLPYEIDGIVIKVDSLDQQEELGFTAKSPRWAIAYKFPAEEVVTKLLDIELNVGRTGVITPTAILEPVKVAGTTVSRASLHNEDLIKEKDIRILDKVVVKKAGDIIPEVVNVLVDQRTGEEKEFSMPTECPECGSELVRIEGEVALRCINPECPAQIREGLIHFVSRNAMNIDGLGERVITQLFEENLVRNVADLYKLTKERVIQLERMGEKSTENLISSIQKSKENSLERLLFGLGIRFIGSKAAKTLAMHFESLENLKKASKEELLAVDEIGEKMADAVITYFHKEEMLELLNELQELGVNTLYKGPKKVKAEDSDSYFAGKTIVLTGKLEELSRNEAKAQIEALGGKLTGSVSKNTDLVIAGEAAGSKLTKAQELNIEVWNEEQLMGELKK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGATAAAGA AACCGCTAAA CAACGTGCCG
     2041 AGGAGCTGCG CCGTACCATC AACAAGTACA GCTATGAGTA CTATACCCTC GATGAGCCTT
     2101 CAGTTCCGGA TGCGGAGTAC GATCGTCTGA TGCAAGAACT GATCGCGATT GAGGAGGAAC
     2161 ACCCGGATCT GCGTACCCCG GATAGCCCGA CGCAACGCGT AGGTGGTGCA GTGCTGGAAG
     2221 CGTTCCAGAA AGTTACCCAC GGCACGCCGA TGCTGAGCTT GGGTAACGCG TTCAACGCGG
     2281 ACGATCTGCG TGATTTTGAC CGCCGTGTTC GTCAGAGCGT CGGTGACGAT GTGGCGTACA
     2341 ACGTGGAGCT GAAAATTGAC GGCCTGGCAG TCTCTCTGCG CTATGAGGAC GGTTACTTCG
     2401 TCCGTGGTGC AACGCGTGGT GACGGTACGA CGGGTGAGGA CATCACCGAG AACCTTAAGA
     2461 CCATTCGTAA TATCCCGCTG AAGATGAATC GCGAGTTGTC TATCGAAGTC CGCGGCGAGG
     2521 CCTACATGCC TAAACGTAGC TTCGAAGCAC TGAACGAGGA GCGCATCAAG AATGAAGAGG
     2581 AGCCATTTGC GAACCCGCGC AACGCGGCCG CCGGTAGCCT GAGACAGCTG GATCCGAAAA
     2641 TTGCAGCCAA GCGTAATTTG GACATTTTCG TGTACTCTAT TGCGGAGCTG GACGAGATGG
     2701 GTGTGGAAAC TCAGTCTCAA GGTCTGGATT TCTTAGACGA ACTGGGCTTT AAAACCAATC
     2761 AAGAGCGTAA GAAATGTGGC AGCATTGAGG AGGTCATTAC TCTGATTGAC GAGTTGCAGG
     2821 CGAAGCGTGC GGATCTGCCA TATGAAATCG ATGGTATCGT GATCAAAGTG GACAGCTTGG
     2881 ATCAACAGGA GGAACTGGGT TTCACCGCCA AGAGCCCGCG TTGGGCAATT GCGTACAAAT
     2941 TCCCAGCGGA AGAGGTCGTG ACTAAACTGC TGGACATCGA GCTGAACGTT GGTCGTACCG
     3001 GTGTCATTAC GCCGACGGCC ATTCTGGAAC CAGTTAAAGT GGCGGGTACC ACGGTGAGCC
     3061 GTGCTAGCCT GCACAATGAG GACTTGATCA AAGAAAAAGA CATTCGTATT CTGGATAAGG
     3121 TGGTCGTGAA AAAAGCAGGC GATATTATTC CGGAAGTGGT TAATGTTCTG GTTGACCAGC
     3181 GTACCGGTGA GGAGAAGGAG TTCAGCATGC CTACCGAGTG CCCGGAGTGC GGCAGCGAGC
     3241 TGGTTCGCAT TGAAGGTGAA GTTGCGCTGC GTTGTATCAA TCCGGAATGC CCGGCACAGA
     3301 TCCGCGAAGG TCTGATTCAC TTCGTGAGCC GTAATGCCAT GAATATTGAT GGTCTGGGCG
     3361 AGCGTGTGAT CACTCAGCTG TTTGAAGAGA ATCTGGTCCG TAACGTTGCC GACCTGTACA
     3421 AACTGACGAA GGAACGCGTA ATCCAGCTGG AGCGTATGGG TGAAAAGAGC ACCGAGAATC
     3481 TGATCAGCAG CATTCAAAAG AGCAAGGAAA ACTCCCTGGA ACGTCTGCTG TTCGGTCTGG
     3541 GCATTAGATT CATCGGCAGC AAGGCGGCGA AAACCCTGGC CATGCACTTT GAGAGCCTGG
     3601 AGAATCTGAA GAAAGCAAGC AAAGAAGAAT TGCTGGCGGT CGACGAGATC GGCGAAAAGA
     3661 TGGCGGATGC GGTGATTACC TATTTCCATA AGGAAGAGAT GTTGGAGCTG CTGAATGAGC
     3721 TGCAAGAATT GGGTGTGAAC ACGCTGTACA AGGGCCCGAA AAAGGTGAAA GCAGAGGACA
     3781 GCGACTCTTA CTTTGCGGGC AAGACCATTG TCCTGACCGG TAAATTAGAG GAGCTGAGCC
     3841 GTAATGAGGC CAAAGCCCAA ATTGAGGCGC TGGGTGGCAA ACTGACCGGT AGCGTGAGCA
     3901 AAAACACCGA CCTGGTGATC GCCGGTGAAG CGGCGGGCAG CAAATTGACG AAAGCGCAGG
     3961 AACTGAACAT TGAGGTGTGG AACGAGGAGC AGCTGATGGG CGAATTGAAG AAGTGAAGAG
     4021 CTTAGAGACC
//