LOCUS       Exported     3145 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3145
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1183..1230
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3078..3094
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3095..3126
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             100..3144
                     /label="dapL"
                     /note="Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate."
                     /translation="MKIEELIAIRRDLHRIPELGFQEFKTQQYLLNVLEQYPQDRIEIEKWRTGLFVKVNGTAPEKMLAYRADIDALSIEEQTGLPFASEHHGNMHACGHDLHMTIALGIIDHFVHHPVKHDLLFLFQPAEEGPGGAEPMLESDVLKKWQPDFITALHIAPELPVGTIATKSGLLFANTSELVIDLEGKGGHAAYPHLAEDMVVAASTLVTQLQTIISRNTDPLDSAVITVGTITGGSAQNIIAETAHLEGTIRTLSEESMKQVKERIEDVVKGIEIGFRCKGKVTYPSVYHQVYNTSGLTEEFMSFVAEHQLATVIEAKEAMTGEDFGYMLKKYPGFMFWLGADSEHGLHHAKLNPDENAIETAVHVMTGYFSVYAN"
     primer_bind     complement(1235..1251)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1318..1906)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2097..2957)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2958..3062)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG AAAATCGAGG AGCTGATCGC TATTCGTCGT GATCTGCACC GCATCCCAGA
       61 ACTGGGTTTT CAAGAGTTCA AAACCCAGCA GTATCTGCTG AACGTGCTGG AACAATACCC
      121 GCAAGACAGA ATTGAGATCG AAAAGTGGCG TACTGGTTTG TTCGTTAAAG TCAATGGTAC
      181 CGCGCCAGAG AAAATGCTGG CGTATCGTGC GGATATCGAC GCATTGAGCA TCGAGGAGCA
      241 AACCGGTCTG CCATTCGCGT CAGAACACCA TGGTAACATG CATGCGTGCG GCCATGATCT
      301 GCACATGACA ATCGCGCTGG GTATTATTGA TCACTTTGTC CACCACCCGG TCAAACATGA
      361 TCTGCTGTTC TTGTTCCAGC CGGCCGAGGA GGGCCCAGGT GGTGCCGAAC CGATGCTGGA
      421 GTCAGATGTC CTGAAAAAAT GGCAGCCGGA CTTCATCACC GCCCTGCATA TCGCGCCGGA
      481 GCTGCCGGTC GGCACGATTG CGACCAAGAG CGGCCTGCTG TTTGCGAATA CGTCAGAGCT
      541 GGTAATTGAT CTTGAAGGCA AGGGTGGTCA CGCGGCTTAT CCGCACTTAG CTGAAGATAT
      601 GGTTGTTGCG GCGTCTACGC TGGTGACCCA ACTGCAGACC ATCATTAGCC GCAATACGGA
      661 CCCGCTGGAC AGCGCAGTGA TCACGGTTGG TACGATCACC GGTGGTAGCG CGCAAAATAT
      721 TATTGCGGAA ACCGCGCATC TGGAGGGTAC TATTCGTACC TTGAGCGAAG AGAGCATGAA
      781 ACAAGTTAAG GAGCGCATTG AGGACGTGGT CAAGGGTATT GAGATCGGTT TCCGCTGTAA
      841 AGGTAAAGTT ACTTACCCGA GCGTCTATCA CCAGGTCTAC AACACGAGCG GTCTGACTGA
      901 GGAATTCATG AGCTTTGTGG CAGAGCATCA ACTGGCGACC GTCATCGAAG CGAAGGAAGC
      961 TATGACCGGT GAGGATTTTG GTTACATGCT GAAGAAATAT CCTGGTTTTA TGTTTTGGTT
     1021 GGGTGCCGAC AGCGAGCACG GCCTGCACCA CGCAAAACTG AACCCGGATG AGAATGCCAT
     1081 TGAAACTGCG GTTCATGTCA TGACGGGTTA TTTTTCTGTC TACGCCAACT GAAGAGCTTA
     1141 GAGACCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1201 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1261 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1321 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1381 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1441 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1501 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1561 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1621 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1681 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1741 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1801 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1861 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     1921 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     1981 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     2041 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     2101 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     2161 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     2221 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     2281 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     2341 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     2401 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2461 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     2521 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     2581 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     2641 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     2701 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     2761 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     2821 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     2881 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     2941 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     3001 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     3061 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     3121 TTGACTTGGT CAGGTGGAGT GGGAG
//