LOCUS       Exported     3691 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3691
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3677
                     /note="An RNase that has endonuclease and 5'-3' exonuclease activity, playing a role in both rRNA and mRNA stability and degradation. Endonuclease activity can cleave within 4 nucleotides of the 5'-end of a triphosphorylated RNA. Endonuclease digestion by the RNase J1/J2 complex occurs at a different site and in some cases more efficiently than J1 or J2 alone. The exonuclease activity of the J1/J2 complex is highly processive on substrates longer than 5 nucleotides, on shorter substrates is distributive. Preferentially cleaves ssRNA, possibly in AU-rich regions. The 5'-exonuclease activity acts on 5'-hydroxyl and 5'-monophosphate but not 5'-triphosphate ends; it can digest through stem-loop structures if they are not too stable. Required for maturation of 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal subunits. Plays a role in the secondary pathway of 23S rRNA 5' end maturation. Probably also participates in processing of pre-scRNA (the precursor of the signal recognition particle RNA). Major RNase that degrades both RNAs of the type I toxin-antitoxin system BsrE/SR5 (PubMed:26940229)."
                     /translation="MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYLVKNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLNIIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIVHTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQVIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPMAALSRIANGTHRQISINPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHVKLATDCGIPEENCFIMDNGEVLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKISAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEV"
                     /label="rnjA"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGTTCGT TAAGAATGAC CAAACGGCGG
     2041 TGTTTGCACT GGGCGGTCTG GGCGAGATTG GCAAAAATAC CTACGCGGTG CAATTCCAGG
     2101 ACGAGATCGT GCTGATCGAT GCCGGCATTA AGTTCCCGGA GGATGAGCTG CTGGGCATTG
     2161 ATTATGTGAT TCCTGATTAC ACCTATTTGG TCAAGAATGA AGATAAGATC AAGGGTCTGT
     2221 TTATCACGCA TGGCCACGAG GATCATATCG GTGGTATCCC GTATCTGCTG CGCCAGGTCA
     2281 ATATCCCAGT GTACGGCGGT AAGCTGGCGA TTGGTCTGCT GCGCAATAAG TTGGAGGAGC
     2341 ATGGTCTGCT GCGTCAAACC AAGCTGAACA TTATCGGTGA GGATGATATC GTCAAGTTTC
     2401 GTAAAACGGC TGTTTCGTTC TTTCGTACGA CTCATAGCAT CCCGGACAGC TACGGTATCG
     2461 TCGTTAAGAC CCCGCCGGGT AACATTGTTC ATACTGGCGA TTTCAAATTC GATTTTACCC
     2521 CTGTGGGTGA GCCGGCTAAT CTGACTAAAA TGGCTGAAAT TGGCAAAGAG GGTGTGCTGT
     2581 GCTTGCTGAG CGATTCCACG AACAGCGAGA ATCCGGAATT CACCATGAGC GAAAGACGTG
     2641 TGGGCGAAAG CATTCATGAC ATCTTTCGCA AGGTGGACGG CCGCATCATC TTTGCGACGT
     2701 TTGCGAGCAA TATCCATCGT CTGCAACAGG TGATTGAAGC AGCCGTCCAG AATGGTCGTA
     2761 AAGTTGCGGT GTTCGGCCGC AGCATGGAGA GCGCGATTGA GATTGGCCAA ACGTTGGGTT
     2821 ACATTAATTG CCCGAAGAAT ACCTTTATCG AACACAATGA AATCAATCGT ATGCCGGCGA
     2881 ATAAAGTCAC GATTCTGTGC ACGGGCAGCC AAGGTGAGCC GATGGCGGCA CTGAGCCGTA
     2941 TTGCCAACGG CACCCACCGC CAAATCTCTA TTAACCCGGG TGACACCGTC GTGTTCAGCT
     3001 CAAGCCCGAT CCCGGGCAAC ACCATTAGCG TTTCACGTAC GATTAATCAA CTGTACCGTG
     3061 CAGGCGCAGA AGTCATTCAC GGTCCGTTAA ACGACATCCA TACCAGCGGC CATGGTGGTC
     3121 AGGAGGAGCA GAAGCTGATG CTGCGTCTGA TCAAACCGAA ATTTTTCATG CCGATTCATG
     3181 GCGAGTACCG CATGCAAAAA ATGCACGTCA AGCTGGCGAC TGACTGCGGT ATCCCGGAAG
     3241 AAAACTGCTT TATTATGGAC AATGGTGAGG TCCTGGCGCT GAAGGGGGAT GAGGCTAGCG
     3301 TGGCGGGCAA AATTCCGAGC GGCAGCGTGT ACATTGACGG CAGCGGTATT GGTGACATCG
     3361 GTAACATCGT TCTGCGTGAC CGTCGTATTC TGAGCGAGGA GGGCCTGGTT ATCGTAGTCG
     3421 TCAGCATTGA TATGGATGAC TTTAAGATCT CTGCCGGTCC GGACCTCATC AGCCGTGGTT
     3481 TTGTGTACAT GCGCGAGTCT GGTGACTTGA TCAACGACGC GCAGGAGTTG ATCAGCAACC
     3541 ATCTGCAAAA GGTTATGGAA CGCAAAACCA CGCAGTGGAG CGAAATCAAA AACGAGATTA
     3601 CCGATACCCT GGCACCGTTC CTGTACGAAA AAACGAAGCG CCGCCCGATG ATCCTGCCGA
     3661 TTATCATGGA AGTGTGAAGA GCTTAGAGAC C
//