LOCUS       Exported     4174 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4174
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4160
                     /note="Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active site of these proteins, thereby interrupting normal cell wall synthesis."
                     /translation="MIQMPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRTIEASRGSILDRKGKVIAEDTATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLDKADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGISFLRDTKRYYPNGVFASNLIGYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNVYLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYYNDLISYAYEPGSTMKIFTLAAAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTYHDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGIDLPGEVSSKINFKYEFDKASTAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISASTAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMAPKDDPELLIYVAVQQPQLKAGQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKAQTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPKADEEVLTNQKVFLKTGGKIKMPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD"
                     /label="pbpB"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGATTCAAAT GCCGAAAAAG AACAAATTCA
     2041 TGAATCGTGG TGCGGCGATC TTGAGCATCT GCTTCGCATT GTTCTTTTTT GTTATCCTGG
     2101 GTCGTATGGC ATACATTCAA ATCACCGGTA AGGCGAATGG CGAAGTTCTC GCAACGAAGG
     2161 CTACCGAGCA GCATGAGAAA AAACGTACTA TCGAAGCGAG CCGCGGTAGC ATCCTGGACC
     2221 GCAAAGGTAA GGTCATTGCA GAGGACACGG CGACCTACAA ACTGATTGCC ATTCTGGACA
     2281 AGAAGATGAC CACGGATGTG AAACATCCGC AGCATGTTGT GAACAAAGAA AAAACCGCAG
     2341 AGGCTCTGAG CAAGGTCATT AATCTGGACA AGGCCGATAT CTTAGATATT TTGAACAAAG
     2401 ACGCTAAGCA AGTTGAGTTC GGTTCCGCGG GCCGTGATAT CACGTACAGC CAAAAGCAAA
     2461 AAATCGAGAA AATGAAATTG CCGGGCATTT CGTTCCTGCG TGATACCAAG CGTTACTACC
     2521 CGAACGGTGT GTTTGCGTCG AATCTGATCG GCTACGCGGA AGTTGATGAA GAAACGAATG
     2581 AGATCTCCGG TGCAATGGGT CTGGAGAAAG TTCTGGACAA ATATTTGAAG GAACGCGACG
     2641 GTTACGTCAC GTACGAGAGC GATAAATCGG GCTGGGAGCT GCCGAATAGC AAAAACAAGA
     2701 TCACCGCCCC GAAAAACGGT GACAACGTGT ACCTGACCAT TGATCAGAAA ATCCAGACCT
     2761 TTCTGGAGGA CTCTATGACG AAGGTGGCCC AAAAGTATAA TCCGAAGAAA ATTATGGCTG
     2821 CGGTCGTCGA CCCAAAGACC GGCAAGGTTT TGGCAATGGG TCAAAGACCG AGCTTTGATC
     2881 CGAACAAACG TGATGTTACC AACTACTACA ACGACTTAAT CAGCTATGCA TATGAGCCGG
     2941 GCTCTACCAT GAAGATTTTT ACCCTGGCAG CTGCCATGCA AGAGAACGTT TTCAACGCCA
     3001 ATGAAAAGTA CAAGTCCGGT ACCTTTGAGG TGGGTGGTGC GCCTGTGAAG GACCATAATA
     3061 ACGGCGTTGG CTGGGGCCCT ACTACGTACC ACGATGGTGT TCTGCGTAGC AGCAACGTCG
     3121 CATTCGCCAA GCTGGCGAAA GAGAAGCTGG GTTATGACCG CCTGAACCAG TATCTGCACA
     3181 AATTTAATTT TTACCAGAAA ACTGGCATCG ATCTGCCGGG CGAAGTATCC AGCAAGATCA
     3241 ACTTTAAATA CGAGTTTGAC AAGGCAAGCA CGGCGTATGG TCAGGCAAGC GCGGTTACGC
     3301 CGATTCAGCA GATCCAAGCT GCGACCGCAA TTGCAAACGA TGGTAAAATG ATGAAGCCGT
     3361 ACGTCATTGA CCACATCGTT GACCCGGATA AAGATAAGAC GATCTATCAG AACAAACCGG
     3421 AATCGGCGGG TACTCCAATT AGCGCAAGCA CTGCGAAGAA GGTTCGTGAT ATCTTGGGCG
     3481 AGGTCGTGAC CAGCAAGATT GGCACCGGCC AGGCCTATAA GATTGAGGGT TTTGACGTTG
     3541 CGGGTAAAAC GGGCACTGCA CAAATCGCCG GTAAAGGCGG TTATCTGGAC GGTACCGACA
     3601 ACTACATCTT TAGCTTCATG GGTATGGCGC CGAAGGACGA TCCGGAGTTG CTGATCTATG
     3661 TTGCAGTCCA GCAACCGCAG CTGAAGGCCG GTCAGAGCAG CAGCGACCCA GTGAGCGAGA
     3721 TCTTCAATCC GACGATGAAG AACAGCTTAC ACTACCTGAA CATTGAGCCG ACCGAAAAAA
     3781 GCGACTCCGA CAAGGAGGAA ACCAAAGCTC AAACGATGCC GGACCTGACG GACCAAACGG
     3841 TGGCGGCGGC GCAGAAAAAA GCAAAGGAAG AGAACCTGAC CCCGATCGTA ATTGGTAGCG
     3901 ACGTTGCGGT GAAAGAGCAG TATCCGAAAG CTGACGAAGA AGTGCTGACC AATCAAAAGG
     3961 TGTTCTTAAA GACCGGTGGT AAGATTAAAA TGCCGGACAT GACCGGTTGG AGCCGTCGCG
     4021 AGGTGCTGCA GTACGGTGAA CTGGCGGGCA TTCACATTGA GGTTAGCGGT CAGGGTTACG
     4081 CAGTGAGCCA GAGCGTTAAG AAAGATAAGG AAATCAAGGA TAAAACCGTT ATCAAGGTCA
     4141 AGTTCAAAAA TCCGGATTGA AGAGCTTAGA GACC
//