LOCUS       Exported     3505 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3505
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1543..1590
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3438..3454
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3455..3486
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3504
                     /label="murE"
                     /note="Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan."
                     /translation="MKLTKLLTYLTTEPSVNDSQDPEITSIEMDSREVKKGSLFVCVKGYTVDGHDFAQKAVENGAAAIVAEREVDVNVPVIIVRQSLRALSVLSDAFYGQPTKKLQLIGITGTNGKTSTTHMVDEILKKAGKRTGLIGTMYMKIGDETLPVKNTTPESVTLQKTFKKMNDKHVDTAIMEVSSHALSLGRVHGCDYDIAVFTNLTQDHLDYHKTMDEYRHAKSLLFSQLGGAFNHEHPKRAVLNADDEASAYFEKVTAAHISTYGIKNDADVMAKNISITAQGTSFDLVTNKGTKHITMSLVGQFNVYNVLAAVATCIAAGIPFEIITEAVEELHGVRGRFELVNQQQEFPVIVDYAHTPDSLENVLETCRDMTEGKLFVVVGCGGDRDKTKRPKMAKIAVELADEPIFTSDNPRSEDPRAILRDMEAGVENAYYHSIANREQAIFFAIANAKKGDVVLIAGKGHETYQQIGNETFDFDDAEVAARAIVELNKNKTNS"
     primer_bind     complement(1595..1611)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1678..2266)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2457..3317)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3318..3422)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG AAACTGACCA AACTGCTGAC CTATCTGACC ACCGAACCGT CAGTGAATGA
       61 TTCTCAAGAC CCGGAAATTA CCAGCATTGA GATGGACAGC CGTGAAGTCA AGAAAGGTAG
      121 CCTGTTTGTC TGTGTCAAAG GCTACACCGT TGACGGTCAC GATTTCGCGC AAAAAGCAGT
      181 AGAAAATGGC GCAGCGGCTA TCGTTGCGGA ACGTGAAGTC GATGTAAACG TTCCGGTGAT
      241 CATTGTCAGA CAAAGCCTGC GTGCCCTGAG CGTCCTGTCT GACGCGTTCT ACGGCCAGCC
      301 GACCAAAAAA CTCCAGCTGA TTGGCATTAC GGGCACCAAC GGTAAGACGT CCACCACCCA
      361 CATGGTAGAT GAAATTCTGA AAAAGGCGGG CAAGCGTACG GGTCTGATTG GCACCATGTA
      421 CATGAAGATT GGTGATGAAA CCCTGCCGGT TAAAAACACG ACTCCGGAAA GCGTCACCCT
      481 GCAAAAAACG TTCAAGAAGA TGAACGATAA ACACGTTGAT ACCGCGATCA TGGAGGTGAG
      541 CAGCCATGCG CTGAGCTTAG GTCGTGTTCA TGGTTGCGAT TACGATATTG CGGTGTTCAC
      601 GAATCTGACC CAGGACCACC TGGATTATCA TAAAACCATG GACGAATACC GCCACGCAAA
      661 AAGCCTTTTG TTTAGCCAAC TGGGCGGCGC GTTCAACCAT GAGCATCCGA AACGCGCGGT
      721 TCTGAACGCT GACGATGAGG CGTCAGCCTA CTTTGAGAAG GTCACCGCGG CGCATATTTC
      781 CACCTACGGT ATTAAAAACG ATGCGGATGT AATGGCCAAG AACATTAGCA TTACTGCTCA
      841 AGGTACCAGC TTTGATCTGG TCACGAATAA GGGTACCAAG CATATCACCA TGAGCCTGGT
      901 GGGTCAGTTT AATGTTTATA ATGTGCTGGC TGCAGTCGCA ACGTGCATTG CGGCCGGCAT
      961 CCCGTTTGAG ATTATCACCG AGGCGGTCGA GGAACTGCAT GGTGTACGCG GCCGCTTCGA
     1021 ACTGGTGAAC CAACAGCAGG AGTTTCCGGT TATCGTGGAT TACGCGCACA CCCCAGACAG
     1081 CCTGGAGAAC GTTTTAGAAA CTTGCCGTGA CATGACGGAA GGTAAGCTGT TTGTCGTCGT
     1141 TGGTTGCGGT GGTGATCGTG ACAAAACCAA GCGCCCGAAG ATGGCGAAAA TTGCGGTCGA
     1201 GCTGGCAGAC GAGCCTATCT TCACCAGCGA CAACCCGCGC TCCGAGGACC CGCGCGCAAT
     1261 TCTGCGCGAT ATGGAGGCAG GCGTGGAGAA CGCGTACTAC CACTCGATCG CGAACCGTGA
     1321 ACAGGCGATC TTTTTCGCGA TCGCAAACGC CAAAAAGGGT GATGTGGTCC TGATTGCTGG
     1381 TAAAGGTCAC GAAACCTACC AACAAATTGG TAACGAAACC TTCGACTTTG ATGACGCCGA
     1441 AGTGGCCGCG CGCGCAATCG TCGAGCTGAA TAAGAACAAA ACGAATTCCT GAAGAGCTTA
     1501 GAGACCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1561 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1621 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1681 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1741 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1801 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1861 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1921 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1981 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     2041 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     2101 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     2161 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     2221 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     2281 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     2341 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     2401 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     2461 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     2521 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     2581 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     2641 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     2701 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     2761 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2821 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     2881 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     2941 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     3001 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     3061 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     3121 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     3181 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     3241 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     3301 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     3361 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     3421 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     3481 TTGACTTGGT CAGGTGGAGT GGGAG
//