LOCUS       Exported     3346 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3346
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3332
                     /label="ftsA"
                     /note="Cell division protein that is required for the assembly of the Z ring (PubMed:16159787). May serve as a membrane anchor for the Z ring (By similarity). Binds and hydrolyzes ATP (PubMed:11298280). Also involved in sporulation (Probable)."
                     /translation="MNNNELYVSLDIGTSNTKVIVGEMTDDSLNIIGVGNVPSEGLKKGSIVDIDETVHSIRKAFDQAERMVGFPLRKAIVGVNGNYINIQDTNGVVAVSSENKEIQVEDVRRVMEAAQVVSVPHEQLIVDVIPKQFIVDGRDEITDPKKMLGVRLEVEGTLITGSKTILHNLLRCVERAGIEITDICLQPLAAGSAALSKDEKNLGVALIDIGGGSTTIAVFQNGHLTSTRVIPLGGENITKDISIGLRTSTEEAERVKKQLGHAYYDEASEDEIFEVTVIGTNQKQTFTQQEAANIIEARVEEILEIVSEELRSMGITDLPGGFVLTGGQAAMPGVMSLAQDVLQNNVRVASPNYIGVRDPQYMTGVGLIQFACRNARIQGRKIGFKMPEEAIQEIAVSSSEEQEQHHHQNEVQQRPKGKQKTQAEHNKQSKMKKLLSMFWE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAATAACAA CGAGCTGTAC GTTAGCCTGG
     2041 ATATCGGCAC GAGCAACACT AAGGTTATCG TGGGTGAGAT GACGGATGAC AGCCTGAATA
     2101 TCATTGGTGT GGGCAACGTG CCATCTGAAG GTCTGAAAAA GGGCAGCATC GTTGACATTG
     2161 ACGAAACCGT GCATAGCATC CGTAAAGCGT TCGACCAAGC AGAGCGTATG GTGGGCTTCC
     2221 CTCTGCGTAA GGCCATCGTG GGTGTCAACG GTAACTACAT TAACATCCAG GACACCAATG
     2281 GCGTGGTCGC GGTCAGCAGC GAGAATAAAG AAATTCAGGT TGAGGATGTG CGCCGTGTCA
     2341 TGGAGGCAGC ACAGGTCGTT AGCGTTCCGC ATGAGCAGCT GATTGTTGAC GTGATCCCGA
     2401 AACAGTTTAT CGTCGACGGT CGTGACGAAA TCACCGATCC GAAAAAGATG TTGGGTGTGC
     2461 GCCTGGAAGT TGAGGGTACC CTGATCACGG GTTCCAAAAC CATTCTGCAC AATCTGCTGC
     2521 GCTGCGTGGA GCGCGCCGGT ATCGAGATCA CCGACATCTG CCTGCAGCCG CTGGCGGCCG
     2581 GTAGCGCGGC GCTGAGCAAG GACGAGAAAA ACCTGGGCGT GGCGCTGATT GATATCGGTG
     2641 GCGGTAGCAC GACGATCGCG GTGTTCCAGA ACGGTCACCT GACGAGCACG CGTGTTATCC
     2701 CGCTGGGTGG TGAGAATATC ACCAAGGACA TTAGCATTGG TCTGCGTACG AGCACTGAGG
     2761 AGGCGGAACG TGTGAAAAAG CAATTGGGTC ACGCATATTA CGATGAAGCG AGCGAGGACG
     2821 AAATCTTCGA AGTGACTGTG ATCGGCACGA ATCAGAAGCA AACGTTCACC CAACAGGAGG
     2881 CGGCGAACAT CATTGAGGCG CGTGTGGAGG AGATTCTGGA GATCGTCAGC GAGGAGCTGC
     2941 GCAGCATGGG CATCACGGAT CTGCCGGGCG GTTTTGTGCT GACGGGCGGC CAAGCCGCCA
     3001 TGCCGGGCGT TATGAGCCTG GCCCAAGATG TGCTGCAGAA TAACGTTCGC GTGGCGTCCC
     3061 CGAATTATAT CGGTGTCCGC GACCCGCAGT ATATGACCGG TGTGGGCCTG ATTCAGTTCG
     3121 CATGCCGCAA TGCCCGCATT CAAGGTCGTA AGATTGGTTT TAAGATGCCG GAAGAGGCCA
     3181 TTCAAGAGAT TGCGGTTAGC AGCAGCGAGG AACAGGAGCA GCACCACCAC CAGAATGAGG
     3241 TTCAGCAGCG TCCGAAGGGT AAACAAAAAA CGCAAGCGGA ACATAACAAA CAGAGCAAAA
     3301 TGAAAAAGCT GCTGAGCATG TTCTGGGAGT GAAGAGCTTA GAGACC
//