LOCUS       Exported     4789 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4789
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4775
                     /label="ileS"
                     /note="Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)."
                     /translation="MDFKDTLLMPKTDFPMRGNLPNREPDIQKKWEEEDIYRLVQERTKDRPKFVLHDGPPYANGDIHMGHALNKILKDFIVRYKSMSGYNAPYVPGWDTHGLPIETALTKNKKVNRKEMSVAEFRKLCEEYAWKQIEGQREQFKRLGVRGDWENPYVTLKPEYEAQQIRVFGEMAKRGYIYKGLKPVNWSPSSESALAEAEIEYQDKRSASIYVAFGVKDGKGVLENGERIIIWTTTPWTIPANLGISVHPDLEYSVIAVGEDRFVVASALVENVASACGFDQYEVTRTVKGKDLENIIAEHPLYGRDSLVMLGEHVTTDAGTGCVHTAPGHGEDDFIIGQKYGLDVLCPVDEKGVMTSEAPGFEGMFYDDANKAITQQLDEKGALVKLEFITHSYPHDWRTKKPTIFRATAQWFASIKDFRSDLLDAIKETKWVPEWGEQRLHNMVRDRGDWCISRQRAWGVPIPVFYAENGEPVITDETIEHVSELFRQHGSNIWFEKEAKDLLPEGFTHPGSPNGTFTKEQDIMDVWFDSGSSHQAVLEERDDLVRPADLYLEGSDQYRGWFNSSLSTAVAVTGKAPYKGVLSHGFALDGEGRKMSKSIGNVVVPAKVMKQLGADILRLWVSSVDYQADVRVSDAILKQVAEVYRKIRNTFRFLHGNLFDFDPKTNAVAVEDLREVDQYMLIKLNKLIDKVKKAYDEYEFAVVYHSIHNFCTIELSSFYLDFAKDIVYIEHADHPDRRSMQTVFYETLLALVKLSAPILPHTADELWSHLTFVEEQSVQLTDMPETITVPNSEATEEKFDRFMALRDDVLKALETARNEKIIGKSLEANLKLYPNKENKELLASIKENLSQLFIVSELTISEENEAPNDAQSFATGKIAVEKAEGEMCERSRVISKDVGANPKYPTLSLRNAEIVEKYYQK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGACTTCAA GGATACCTTG TTGATGCCAA
     2041 AGACGGACTT CCCGATGCGC GGCAATCTGC CGAATCGTGA ACCTGATATC CAAAAGAAGT
     2101 GGGAGGAAGA AGATATCTAC CGTCTGGTCC AGGAACGTAC CAAGGACCGC CCGAAATTCG
     2161 TGTTGCATGA CGGCCCGCCG TATGCTAATG GTGATATTCA CATGGGTCAT GCATTGAACA
     2221 AAATTCTGAA AGATTTCATT GTCCGTTATA AGAGCATGAG CGGTTACAAT GCCCCGTACG
     2281 TGCCGGGTTG GGATACGCAT GGCCTGCCGA TCGAAACCGC ACTGACCAAA AATAAAAAAG
     2341 TTAATCGTAA AGAAATGAGC GTTGCGGAGT TCCGTAAGCT CTGCGAAGAA TACGCCTGGA
     2401 AACAGATCGA GGGTCAGCGT GAGCAATTCA AACGTTTGGG TGTGCGTGGC GACTGGGAAA
     2461 ATCCGTACGT TACTTTGAAG CCGGAATACG AAGCACAGCA AATTCGCGTT TTTGGTGAAA
     2521 TGGCGAAGCG TGGCTATATT TACAAAGGTC TGAAACCGGT GAACTGGAGC CCAAGCTCCG
     2581 AGAGCGCCCT GGCCGAGGCG GAAATTGAGT ATCAAGACAA ACGTAGCGCC AGCATTTATG
     2641 TCGCGTTTGG TGTTAAGGAC GGTAAGGGTG TGCTGGAAAA TGGTGAGCGT ATTATTATTT
     2701 GGACTACCAC GCCATGGACC ATCCCTGCTA ACCTGGGTAT TAGCGTTCAC CCGGATCTGG
     2761 AATACTCTGT AATCGCTGTC GGCGAAGATC GTTTCGTAGT CGCGAGCGCG CTGGTGGAGA
     2821 ATGTTGCTAG CGCATGCGGT TTCGACCAGT ATGAGGTGAC GCGCACCGTA AAGGGTAAGG
     2881 ATCTGGAAAA TATTATTGCC GAGCACCCGC TGTACGGTCG TGACTCTCTG GTTATGCTGG
     2941 GCGAGCACGT GACCACCGAC GCCGGTACCG GCTGCGTTCA CACCGCGCCG GGCCATGGTG
     3001 AGGACGACTT CATTATCGGT CAAAAGTACG GCCTGGACGT TCTGTGTCCG GTGGATGAGA
     3061 AAGGTGTCAT GACGAGCGAA GCCCCGGGTT TTGAGGGTAT GTTCTACGAC GACGCCAACA
     3121 AGGCGATCAC CCAACAGCTG GATGAAAAGG GTGCGCTGGT GAAGTTGGAA TTCATTACTC
     3181 ATAGCTACCC TCACGATTGG CGTACGAAGA AGCCTACCAT CTTTAGAGCC ACCGCGCAGT
     3241 GGTTCGCCTC CATCAAGGAT TTCCGTTCTG ATCTGCTGGA CGCCATTAAA GAAACCAAAT
     3301 GGGTTCCAGA GTGGGGTGAG CAGCGTCTGC ACAACATGGT GCGCGACCGC GGCGACTGGT
     3361 GTATTAGCCG TCAGCGCGCC TGGGGTGTGC CTATTCCGGT TTTTTATGCG GAGAATGGTG
     3421 AGCCTGTGAT TACCGACGAA ACCATTGAGC ACGTTTCTGA GCTGTTTCGC CAACATGGTA
     3481 GCAACATCTG GTTTGAAAAA GAGGCGAAAG ATCTGCTGCC GGAGGGTTTC ACCCACCCGG
     3541 GTAGCCCGAA TGGCACCTTT ACGAAGGAAC AGGATATCAT GGATGTTTGG TTCGACAGCG
     3601 GCAGCAGCCA TCAAGCTGTC TTGGAGGAAC GTGATGACCT GGTTCGCCCG GCGGACCTGT
     3661 ATCTGGAGGG CAGCGATCAA TACCGCGGTT GGTTCAATAG CAGCCTGAGC ACCGCAGTGG
     3721 CTGTCACCGG CAAGGCACCG TACAAAGGCG TGCTGAGCCA CGGCTTTGCT CTGGATGGCG
     3781 AGGGCCGTAA GATGAGCAAG AGCATTGGTA ACGTCGTGGT TCCTGCCAAA GTTATGAAAC
     3841 AGCTGGGTGC AGATATTCTG CGCTTGTGGG TGTCCAGCGT GGATTATCAG GCAGATGTCC
     3901 GTGTCAGCGA CGCCATCCTG AAGCAAGTCG CGGAGGTGTA CCGTAAGATC CGTAACACGT
     3961 TCCGTTTTCT GCACGGTAAT CTGTTCGACT TCGACCCGAA AACCAACGCG GTTGCAGTTG
     4021 AAGATCTGCG TGAGGTCGAC CAGTACATGC TGATCAAGTT GAATAAACTG ATCGACAAGG
     4081 TGAAGAAAGC CTACGATGAA TATGAATTTG CGGTGGTTTA TCATTCGATT CATAATTTTT
     4141 GTACCATTGA GCTGAGCTCC TTCTATTTGG ATTTCGCTAA AGACATCGTT TATATTGAAC
     4201 ACGCGGACCA CCCGGATCGT CGCAGCATGC AAACCGTGTT CTATGAAACC CTGCTCGCTT
     4261 TGGTCAAGCT GAGCGCACCG ATCCTGCCGC ACACTGCCGA CGAGCTGTGG AGCCATCTGA
     4321 CCTTCGTGGA GGAGCAATCC GTGCAGCTGA CCGATATGCC AGAAACCATC ACGGTTCCGA
     4381 ATAGCGAAGC CACCGAGGAG AAATTTGACC GCTTTATGGC CTTGCGTGAT GATGTTCTGA
     4441 AGGCTCTGGA AACCGCACGT AACGAGAAAA TCATCGGCAA ATCGCTGGAG GCAAATCTCA
     4501 AGTTATACCC GAACAAAGAG AACAAAGAGC TGCTGGCAAG CATTAAAGAG AATCTGAGCC
     4561 AACTGTTCAT CGTGTCGGAG CTGACCATTA GCGAGGAGAA TGAAGCGCCG AACGACGCTC
     4621 AGTCCTTTGC GACGGGCAAA ATCGCGGTCG AGAAGGCCGA GGGCGAGATG TGCGAGCGTT
     4681 CCCGTGTCAT CAGCAAAGAC GTAGGTGCGA ACCCGAAGTA TCCAACCCTG AGCCTGCGCA
     4741 ACGCTGAAAT CGTCGAGAAG TACTACCAGA AGTGAAGAGC TTAGAGACC
//