LOCUS       Exported     4441 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4441
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4427
                     /label="priA"
                     /note="Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA."
                     /translation="MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEELMILSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHETLKLIQRHVQKGDIDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQKGLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAIVLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVELMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRFFGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEVAKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMNPYMMM"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAACTTTGC GGAAGTCATT GTGGACGTTT
     2041 CAACGAAGAA TATTGATCGT CCTTTTGACT ACAAAATTCC GGACCACCTG AAGGGTATGA
     2101 TTAAAACCGG TATGCGCGTC ATCGTTCCGT TCGGCCCGCG TAAAATTCAG GGCTTCGTGA
     2161 CGGCTGTTAA GGAAGCAAGC GACCTGAGCG GTAAGTCTGT AAAGGAGGTC GAAGATCTGC
     2221 TCGATCTGAC GCCGGTGCTG ACGGAGGAGC TGATGATCCT TAGCAGCTGG TTAAGCGATA
     2281 AAACGCTTAG CTTCAAGATT ACCGCGCTGC AAGCCATGCT GCCGGCAGCG CTGAAAGCAA
     2341 AGTATGAGAA GGAGCTGAAA ATTGCTCATG GTGCGGACTT GCCGCCTCAG GTGGAGCGTC
     2401 TGTTTTCCGA AACCAAGACG CTGCTGTATA GCGACATTCC GGATCATGAA ACGTTGAAGC
     2461 TGATCCAGCG CCATGTTCAA AAGGGTGACA TCGATGTCAC GTATAAAGTT GCTCAGAAAA
     2521 CGAATAAGAA GATGGTTCGT CATATCCAGG CAAATGCGAG CAAGGAGGAG CTGGCGAAAC
     2581 AGGCGGAAGG CCTGAGCCGT CAAGCAGCTA AGCAACAGGC GATCCTGCAC TTTTTAATCA
     2641 GCGAACCGGA GGGTGTGAAG ATCCCGGCTG CCGAGCTGTG TAAAAAGACG GATACCTCCA
     2701 GCGCGACCAT TAAAACCCTG ATTCAAAAGG GTCTGCTGAA AGAGTCTTAT GAGGAAGTGT
     2761 ATCGCGACCC GTATCAGGAC AAAATGTTCA AGAAAACGGA ACCGCTGCCG CTGACCGACG
     2821 AGCAGCGTGC AGCCTTTGAG CCGATTCGTG AAACCCTGGA CTCAGATGAG CACAAGGTCT
     2881 TTCTGCTGCA CGGTGTTACC GGTTCCGGCA AGACCGAAAT CTATCTGCAG AGCATTGAGA
     2941 AGGTCCTGGC CAAAGGTAAG GAGGCAATCG TGCTGGTGCC GGAAATTTCC CTGACTCCTC
     3001 AAATGGTTAA CCGTTTTAAG GGTAGATTCG GTTCACAGGT TGCGGTAATG CACAGCGGCC
     3061 TGTCTACTGG TGAGAAATAT GATGAGTGGC GCAAAATCCA TCGTAAAGAA GTACGTCTGG
     3121 TCGTGGGTGC GCGTTCTGCG ATCTTCGCGC CGTTCGAGAA TCTGGGTATG ATTATCATTG
     3181 ACGAGGAGCA TGAGAGCTCC TACAAACAGG AGGAAATGCC GCGTTACCAT GCTAAAGAGG
     3241 TTGCCATCAA GCGTGCGGAA CACCACAGCT GTCCAGTTGT GCTGGGTAGC GCCACCCCGA
     3301 CCTTGGAGAG CTATGCCCGT GCGCAGAAGG GTGTATACGA GCTGCTGTCC TTGAAGCACC
     3361 GTGTGAATCA CCGTGTCATG CCGGAGGTTA GCCTGGTCGA CATGCGCGAG GAGCTGCGTA
     3421 ACGGCAACCG TAGCATGTTT AGCGTTGAGT TAATGGAAAA ACTCGAGGAA ACGATTGCCA
     3481 AAGGTGAGCA AGCTGTGCTG TTCTTGAATA AACGTGGTTA CAGCTCCTTC GTCATGTGTC
     3541 GCGACTGCGG CTACGTGCCG CAGTGCCCGC ACTGTGATAT CAGCATGACC TATCATCGTT
     3601 ATGGCCAGCG TCTGAAATGC CACTATTGCG GCCATGAGGA GCCGGTACCG CATACGTGTC
     3661 CAGAATGCGC CAGCGAACAC ATTCGTTTCT TCGGTACGGG TACGCAGCGT GTCGAAGAAG
     3721 AGTTAACCAA AGTTCTGCCG AGCGCGCGTG TCATCCGTAT GGATGTCGAC ACGACTAGCC
     3781 GTAAGGGTGC CCATGAAAAG CTGCTGAGCG CATTTGGTGA AGGTAAAGCG GACATCCTGC
     3841 TGGGTACCCA GATGATCGCG AAAGGCCTGG ACTTTCCGAA TGTCACCCTG GTCGGCGTCT
     3901 TGAGCGCTGA TACCACCCTG CACATCCCGG ACTTCCGCAG CGCTGAAAAG ACCTTTCAGT
     3961 TGCTGACCCA GGTGAGCGGT CGTGCGGGCC GTCATGAGAA ACCGGGCCAT GTTATCATTC
     4021 AGACCTATAC CCCATCTCAC TACAGCATTC AATTGACCAA AACCCATGAT TATGAGACTT
     4081 TTTATCAACA CGAGATGGCA CACCGTCGTG AGCAGAGCTA CCCGCCGTAC TACTACCTGG
     4141 CACTGGTCAC GGTTAGCCAC GAGGAAGTCG CGAAGGCAGC GGTCACGGCG GAGAAAATTG
     4201 CCCATTTCCT GAAAGCAAAC TGCGGCGCAG ATACCAAGAT CCTGGGCCCG AGCGCGTCGC
     4261 CGATCGCCCG TATCAAAGAC CGCTACCGCT ACCAGTGTGT CATTAAATAC AAGCAAGAAA
     4321 CGCAGCTGAG CGCGCTGCTG AAGAAAATCC TGGAGCATTA CAAACGTGAA ATCGAACAAA
     4381 AGCACGTTAT GATTAGCATT GACATGAATC CGTATATGAT GATGTGAAGA GCTTAGAGAC
     4441 C
//