LOCUS       Exported     2902 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2902
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      940..987
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2835..2851
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2852..2883
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..2901
                     /note="Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity)."
                     /translation="MAITAQQVKELREKTGAGMMDCKKALTETDGDMDKAIDLLREKGIAKAAKKADRIAAEGSTLIKTDGNKGVILEVNSETDFVAKNEGFKELLNTLADHLLANTPADVEEAMGQKMENGSTVEEYITSAVAKIGEKITLRRFTVLTKDDSSAFGAYLHMGGRIGVLTVLNGTTDEETAKDIAMHVAAVNPRYISRDQVSEEETNHERQILTQQALQEGKPENIVAKMVEGRLNKFFEEICLLDQAFVKNPDEKVKQVIAAKNATVQTFVRYEVGEGIEKRQENFAEEVMNQVKK"
                     /label="tsf"
     primer_bind     complement(992..1008)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1075..1663)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1854..2714)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2715..2819)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG GCCATCACCG CGCAACAGGT TAAAGAACTG CGTGAAAAAA CCGGTGCCGG
       61 CATGATGGAT TGTAAAAAAG CTCTGACCGA AACCGACGGC GATATGGATA AGGCGATTGA
      121 TTTGCTGCGC GAGAAGGGTA TCGCGAAGGC AGCGAAGAAA GCGGACCGTA TCGCGGCAGA
      181 GGGTAGCACC CTGATCAAGA CCGATGGTAA TAAAGGTGTG ATTTTGGAAG TCAACAGCGA
      241 AACCGACTTT GTGGCGAAAA ACGAAGGCTT CAAAGAACTG CTGAACACGT TGGCCGATCA
      301 TCTGCTCGCG AATACCCCGG CCGATGTCGA AGAAGCCATG GGTCAAAAAA TGGAGAATGG
      361 TAGCACGGTA GAAGAATACA TCACGAGCGC AGTCGCGAAG ATCGGTGAAA AAATTACGCT
      421 GCGTCGTTTC ACGGTTCTGA CCAAAGACGA CAGCTCGGCC TTCGGTGCTT ATCTGCACAT
      481 GGGTGGTCGT ATCGGTGTGC TGACGGTGCT GAACGGTACG ACCGATGAGG AAACCGCAAA
      541 AGATATCGCT ATGCACGTCG CGGCGGTTAA TCCGCGTTAC ATTAGCCGCG ACCAAGTCAG
      601 CGAGGAAGAA ACGAACCACG AGCGTCAAAT CTTGACCCAG CAGGCCCTGC AAGAAGGCAA
      661 GCCGGAGAAT ATCGTTGCCA AGATGGTTGA GGGTCGTCTG AACAAATTCT TCGAAGAAAT
      721 TTGCTTGCTG GACCAGGCGT TCGTCAAGAA CCCGGACGAG AAGGTGAAAC AAGTTATCGC
      781 GGCAAAGAAC GCGACCGTCC AAACCTTCGT TCGCTATGAA GTGGGTGAGG GTATTGAGAA
      841 ACGTCAGGAA AATTTTGCGG AGGAGGTAAT GAACCAAGTT AAGAAGTGAA GAGCTTAGAG
      901 ACCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      961 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1021 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1081 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1141 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1261 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1321 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1381 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1441 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1501 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1561 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1621 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1681 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     1741 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     1801 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     1861 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     1921 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     1981 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2041 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2101 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2161 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2221 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2281 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2341 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2401 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2461 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2521 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2581 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2641 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     2701 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     2761 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     2821 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     2881 ACTTGGTCAG GTGGAGTGGG AG
//