LOCUS       Exported     2830 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2830
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      868..915
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2763..2779
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2780..2811
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..2829
                     /note=""
                     /translation="MVDMKQRILTGVLAAIVFLFLVIVGKLPFTILIYAMGSVALFELLRMKKLKLVSLPGLIGLLLLWMFLLPSQYSFFEADGISKMEIALFAVLLLLTYTVLVKNTFTFDEVGFITLAAIYIGMCFHYFIEIRNLDQYGLTYIFYACVVIWSTDSGAYFVGKSLGKRKLWPEISPNKTVEGFAGGIVIALVLATIFQLVAQLPIPYIYLLLITLFLSVFGQLGDLVESALKRHYDVKDSGNILPGHGGILDRFDSFLFVMPFLYFLLALFS"
                     /label="cdsA"
     primer_bind     complement(920..936)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1003..1591)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1782..2642)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2643..2747)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG GTTGATATGA AGCAGCGTAT TCTGACGGGC GTGCTGGCAG CAATCGTGTT
       61 TCTGTTCTTG GTGATCGTTG GTAAGCTGCC GTTTACTATC CTGATCTACG CCATGGGCAG
      121 CGTTGCTCTG TTTGAATTGT TGCGTATGAA GAAACTGAAA CTGGTTAGCC TGCCGGGTCT
      181 GATCGGTCTG CTGCTGCTGT GGATGTTCCT GCTGCCGAGC CAGTATAGCT TTTTTGAGGC
      241 CGACGGTATT AGCAAAATGG AGATTGCCCT CTTCGCCGTT CTGTTGCTGT TGACGTACAC
      301 CGTCCTGGTC AAAAACACCT TTACGTTCGA TGAAGTTGGT TTTATCACGC TGGCGGCCAT
      361 TTACATTGGC ATGTGTTTCC ACTACTTTAT CGAGATCCGC AACCTGGATC AGTACGGTCT
      421 GACCTATATT TTCTATGCAT GCGTCGTCAT CTGGAGCACG GACAGCGGTG CGTACTTCGT
      481 GGGCAAGTCT TTGGGCAAGC GCAAACTGTG GCCGGAAATC AGCCCGAACA AGACCGTTGA
      541 GGGCTTTGCT GGTGGTATTG TCATTGCTCT CGTGCTGGCG ACGATTTTCC AACTGGTGGC
      601 TCAACTGCCG ATCCCTTATA TCTATCTCCT GCTGATTACC CTGTTTCTGA GCGTTTTTGG
      661 CCAATTGGGT GACCTGGTTG AGAGCGCGCT GAAACGTCAC TATGACGTTA AAGATAGCGG
      721 CAACATTCTG CCGGGTCACG GTGGTATCCT GGACCGCTTT GATTCGTTTT TGTTCGTCAT
      781 GCCGTTCTTG TATTTTTTGT TGGCACTGTT CTCCTGAAGA GCTTAGAGAC CGCTGAGGTG
      841 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
      901 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
      961 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1021 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1081 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1141 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1201 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1261 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1321 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1381 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1441 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1501 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1561 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     1621 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     1681 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     1741 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     1801 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     1861 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     1921 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     1981 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2041 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2101 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2161 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2221 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2281 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2341 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     2401 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     2461 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     2521 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     2581 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     2641 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     2701 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     2761 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT
     2821 GGAGTGGGAG
//