LOCUS Exported 3172 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3172 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1210..1257 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3105..3121 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3122..3153 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3171 /label="dxr" /note="Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP)." /translation="MKNICLLGATGSIGEQTLDVLRAHQDQFQLVSMSFGRNIDKAVPMIEVFQPKFVSVGDLDTYHKLKQMSFSFECQIGLGEEGLIEAAVMEEVDIVVNALLGSVGLIPTLKAIEQKKTIALANKETLVTAGHIVKEHAKKYDVPLLPVDSEHSAIFQALQGEQAKNIERLIITASGGSFRDKTREELESVTVEDALKHPNWSMGAKITIDSATMMNKGLEVIEAHWLFDIPYEQIDVVLHKESIIHSMVEFHDKSVIAQLGTPDMRVPIQYALTYPDRLPLPDAKRLELWEIGSLHFEKADFDRFRCLQFAFESGKIGGTMPTVLNAANEVAVAAFLAGKIPFLAIEDCIEKALTRHQLLKKPSLADIQEVDKDTRGYVNSILT" primer_bind complement(1262..1278) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1345..1933) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2124..2984) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2985..3089) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 GTCTCAAATG AAAAATATTT GCTTACTGGG CGCGACGGGC AGCATTGGTG AGCAGACCCT 61 CGATGTTCTC CGTGCGCACC AGGATCAATT TCAACTGGTG AGCATGAGCT TCGGTAGAAA 121 TATCGACAAA GCCGTCCCAA TGATTGAGGT TTTCCAGCCG AAGTTTGTCA GCGTGGGCGA 181 CCTGGACACC TATCACAAAC TGAAACAAAT GAGCTTCAGC TTCGAGTGCC AAATTGGTTT 241 GGGTGAAGAG GGTCTGATCG AAGCGGCAGT CATGGAGGAA GTTGACATCG TAGTTAACGC 301 ATTGCTGGGC AGCGTGGGCC TGATCCCGAC GCTGAAAGCA ATTGAGCAGA AAAAGACGAT 361 CGCACTGGCG AACAAGGAGA CTCTGGTAAC CGCGGGTCAC ATTGTTAAAG AACATGCGAA 421 GAAATACGAC GTCCCGCTGC TGCCGGTGGA TAGCGAACAC AGCGCGATTT TTCAGGCCCT 481 GCAGGGTGAG CAGGCCAAGA ACATTGAACG TCTGATTATT ACCGCGAGCG GCGGTAGCTT 541 TCGTGACAAG ACTCGTGAAG AACTGGAGAG CGTGACCGTT GAAGATGCGC TGAAACATCC 601 GAACTGGAGC ATGGGCGCAA AGATCACCAT CGATAGCGCA ACCATGATGA ACAAGGGTCT 661 GGAGGTGATC GAGGCGCACT GGCTGTTTGA TATTCCGTAT GAACAGATCG ATGTTGTCCT 721 GCATAAAGAG AGCATCATTC ACAGCATGGT TGAGTTCCAT GACAAATCAG TGATCGCTCA 781 GTTGGGTACG CCGGACATGC GTGTTCCGAT CCAGTACGCG CTGACCTACC CGGACCGCCT 841 TCCGTTGCCT GACGCGAAGC GTCTGGAGCT GTGGGAAATC GGCTCCCTGC ATTTCGAGAA 901 AGCGGACTTT GATCGTTTTC GCTGTCTGCA GTTTGCGTTC GAGAGCGGTA AAATTGGTGG 961 TACCATGCCG ACGGTTCTGA ACGCTGCCAA CGAAGTCGCC GTGGCAGCAT TCTTGGCGGG 1021 CAAAATTCCG TTCCTGGCTA TTGAGGACTG CATCGAGAAG GCACTGACCC GTCACCAGCT 1081 GCTGAAAAAA CCGAGCTTAG CCGATATTCA AGAGGTTGAT AAAGACACCC GCGGCTATGT 1141 TAATAGCATT CTGACCTGAA GAGCTTAGAG ACCGCTGAGG TGTCAATCGT CGGAGCCGCT 1201 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT 1261 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG 1321 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 1381 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 1441 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 1501 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 1561 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 1621 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 1681 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 1741 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 1801 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 1861 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 1921 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG 1981 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 2041 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 2101 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 2161 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 2221 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG 2281 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 2341 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 2401 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 2461 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 2521 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 2581 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 2641 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 2701 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 2761 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 2821 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 2881 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 2941 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 3001 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 3061 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG 3121 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG //