LOCUS       Exported     3718 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3718
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3704
                     /label="proS"
                     /note="Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS."
                     /translation="MRQSLTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLAYKVIQNIQQIVREEMEKIDAVEMLMPALQQAETWQESGRWYTYGPELMRLKDRHGREFALGATHEEVITSLVRDEVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHASAESLDETYQKMYEAYSNIFARCGINVRPVIADSGAMGGKDTHEFMALSAIGEDTIAYSDESQYAANIEMAEVLHQEVPSDEEPKALEKVHTPNVKTIEELTAFLQVSAEACIKSVLFKADDRFVLVLVRGDHEVNDIKVKNLLHAEVVELATHEEVIQQLGTEPGFVGPVGIHQDVEVYADQAVKAMVNAVAGANEGDHHYKNVNVNRDAQIKEFADLRFIKEGDPSPDGKGTIRFAEGIEVGQVFKLGTRYSEAMNATYLDENGRAQPMLMGCYGIGVSRTLSAIAEQHHDEKGLIWPKSVAPYDLHILALNMKNDGQRELAEKLYADLKAEGYEVLYDDRAERAGVKFADSDLIGLPIRITVGKRADEGIVEVKIRQTGESTEISVDELSAFISKQ"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCGTCAGTC CCTGACCCTC ATCCCGACGC
     2041 TGCGTGAAGT GCCGGCCGAT GCGGAGGCCA AGAGCCACCA GCTGCTGCTG CGTGCCGGTT
     2101 TCATTCGCCA AAATACGAGC GGTGTGTACA GCTATATGCC GCTGGCTTAC AAGGTGATTC
     2161 AGAATATTCA GCAGATCGTG CGTGAGGAAA TGGAAAAGAT TGATGCGGTT GAGATGCTGA
     2221 TGCCGGCCCT GCAACAGGCG GAAACGTGGC AGGAATCCGG TCGCTGGTAC ACGTACGGCC
     2281 CGGAACTTAT GCGCCTGAAA GACCGCCATG GTCGCGAATT CGCGCTGGGT GCGACGCATG
     2341 AAGAGGTGAT TACCAGCCTG GTTCGTGATG AGGTCAAGAG CTATAAACGT CTGCCGCTTA
     2401 CGCTGTATCA AATTCAATCT AAATTCCGTG ATGAGAAACG TCCGCGTTTT GGCCTGCTGC
     2461 GTGGCCGTGA ATTTATCATG AAAGACGCAT ACAGCTTCCA TGCAAGCGCG GAGAGCCTGG
     2521 ACGAAACCTA CCAAAAAATG TACGAGGCCT ATAGCAACAT CTTCGCACGT TGTGGTATCA
     2581 ATGTTCGTCC GGTTATTGCT GACAGCGGTG CTATGGGTGG TAAGGACACC CATGAATTTA
     2641 TGGCCCTGTC CGCGATTGGT GAGGACACTA TCGCGTACAG TGATGAGTCC CAGTACGCCG
     2701 CTAATATCGA AATGGCTGAG GTCCTCCATC AGGAGGTTCC GTCTGATGAG GAGCCGAAGG
     2761 CCCTGGAGAA AGTTCATACC CCGAACGTCA AGACGATCGA GGAACTGACG GCCTTTTTGC
     2821 AAGTGAGCGC AGAGGCGTGT ATTAAGTCGG TGCTGTTTAA AGCGGACGAC CGCTTTGTGT
     2881 TAGTCCTGGT CCGTGGCGAT CACGAGGTCA ACGATATCAA GGTGAAAAAT CTGCTGCACG
     2941 CGGAGGTGGT GGAGCTTGCC ACCCACGAAG AAGTGATCCA GCAACTGGGT ACTGAACCGG
     3001 GTTTCGTGGG TCCGGTTGGC ATTCACCAGG ACGTCGAGGT TTACGCAGAT CAAGCGGTTA
     3061 AGGCCATGGT CAACGCGGTG GCGGGCGCTA ATGAAGGCGA TCACCACTAT AAAAACGTTA
     3121 ATGTTAATCG CGACGCGCAG ATCAAAGAAT TCGCGGATCT GCGTTTCATC AAAGAGGGCG
     3181 ACCCGTCCCC GGATGGTAAA GGCACCATCC GTTTCGCAGA GGGCATCGAG GTTGGTCAGG
     3241 TATTCAAGCT GGGCACCCGC TACAGCGAAG CCATGAATGC CACGTACCTG GATGAAAATG
     3301 GCCGTGCGCA ACCGATGTTG ATGGGTTGCT ATGGTATCGG TGTTTCTCGC ACCCTGAGCG
     3361 CAATTGCGGA GCAGCATCAT GACGAGAAAG GTCTTATCTG GCCGAAATCC GTGGCACCGT
     3421 ACGATCTGCA CATCTTAGCG CTGAACATGA AGAATGATGG TCAGCGTGAG CTGGCGGAAA
     3481 AACTGTATGC GGACCTGAAA GCGGAAGGTT ACGAAGTTCT GTATGACGAT CGTGCCGAGC
     3541 GTGCGGGCGT CAAATTCGCG GATAGCGATC TGATTGGTCT GCCGATTAGA ATCACGGTGG
     3601 GTAAACGCGC AGATGAAGGT ATCGTCGAAG TGAAAATCCG TCAAACGGGT GAAAGCACGG
     3661 AGATCAGCGT CGACGAACTG AGCGCCTTTA TTTCAAAGCA GTGAAGAGCT TAGAGACC
//