LOCUS       Exported     4174 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4174
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4160
                     /note="Protects formylmethionyl-tRNA from spontaneoushydrolysis and promotes its binding to the 30S ribosomalsubunits during initiation of protein synthesis. Alsoinvolved in the hydrolysis of GTP during the formation ofthe 70S ribosomal complex"
                     /translation="MAKMRVYEYAKALNVSSKEILTALKNMDLEVNNHMAMLEEKAIKKLDAKYKKGGAAAKSQKPAETNKNKQPQGVNQQSAGNQPNKIRDGKKNDVQNNQFNKNKKNNNNKKNKRNNNNNKNQHQQKPVKPKKELPEKITFSGTLTVGALAEELGKEPSELIKKLMLLGVMATINQELDKDTIELIASEYGVETEEVIVLEETELEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIRKTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAADDGVMPQTVEAINHAKAAEVPIIVAVNKIDKESANPDRVMQELTEYGLVPEAWGGETIFVPLSALTGKGIDELVEMILLVSEVEELKANPNRQAKGTVIEAELDKGRGSVATLLVQTGTLHVGDPIVVGNTFGRVRAMVNDIGRRVKTAGPSTPVEITGLNDVPQAGDQFLVFKDEKTARSVGEARASKQLEEQRSDKAKLSLDDLFEQIKQGDVKDINLIVKADVQGSAEALTAALQKIEVEGVKVKIIHTGVGAITESDIILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIYKVIDEIEAAMKGMLDPEYEEKVIGQVEVRQTFKVSKIGTIAGGYVTEGTITRDSGLRLIRDGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDILEAFVMQEIERT"
                     /label="infB"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGCGAAAAT GCGTGTCTAT GAGTACGCCA
     2041 AGGCCCTGAA CGTGAGCAGC AAAGAAATTC TGACCGCACT GAAGAACATG GATCTGGAGG
     2101 TCAACAACCA CATGGCCATG CTGGAGGAGA AGGCTATTAA AAAGCTGGAC GCGAAATACA
     2161 AGAAGGGTGG CGCGGCTGCA AAGAGCCAAA AACCGGCAGA AACGAATAAG AACAAACAGC
     2221 CGCAGGGTGT CAATCAACAG TCCGCGGGCA ATCAGCCGAA CAAAATCCGT GATGGCAAGA
     2281 AAAATGATGT TCAAAATAAC CAGTTTAATA AGAACAAGAA GAATAACAAT AACAAAAAGA
     2341 ACAAACGTAA CAATAACAAT AACAAGAACC AACACCAGCA GAAGCCGGTC AAGCCGAAGA
     2401 AAGAACTGCC AGAGAAAATC ACCTTCTCCG GTACCCTGAC AGTGGGTGCA CTGGCAGAAG
     2461 AACTGGGTAA AGAGCCGTCA GAATTGATCA AGAAGCTGAT GCTGTTGGGT GTTATGGCAA
     2521 CGATTAATCA AGAACTGGAC AAGGACACGA TCGAATTGAT TGCCAGCGAG TACGGTGTCG
     2581 AAACCGAAGA AGTGATCGTC CTGGAGGAAA CGGAGCTGGA GAAGTATGAG GAACCAGATA
     2641 ATGAAGAGGA CTTGGAAATT CGTCCTCCGG TTGTCACCAT CATGGGTCAC GTGGACCATG
     2701 GCAAGACGAC TCTGCTGGAC AGCATCCGTA AGACCAAAGT AGTTGAGGGT GAGGCTGGTG
     2761 GCATTACTCA ACATATCGGT GCGTATCAGA TCGAGGAAAA TGGTAAAAAG ATTACCTTCC
     2821 TGGACACGCC TGGTCATGCT GCCTTCACCA CCATGCGTGC CCGTGGTGCA GAGGTCACGG
     2881 ATATTACTAT CTTGGTCGTC GCTGCAGATG ACGGCGTGAT GCCGCAGACG GTCGAAGCCA
     2941 TCAATCACGC TAAGGCGGCG GAGGTTCCGA TCATCGTGGC TGTTAACAAG ATCGATAAGG
     3001 AATCTGCGAA TCCAGACCGT GTTATGCAGG AGCTGACCGA GTACGGTCTG GTACCGGAAG
     3061 CGTGGGGCGG TGAAACGATT TTCGTCCCTC TGAGCGCCTT GACTGGTAAA GGCATTGATG
     3121 AGCTGGTTGA GATGATTCTG TTAGTAAGCG AAGTTGAGGA ACTGAAAGCT AACCCTAATC
     3181 GTCAGGCGAA AGGTACTGTC ATCGAGGCGG AGCTGGATAA GGGTCGTGGC TCTGTCGCGA
     3241 CCTTGCTGGT CCAGACCGGC ACGCTGCATG TTGGTGACCC GATCGTCGTG GGTAATACTT
     3301 TCGGTCGCGT ACGTGCCATG GTTAACGATA TTGGCCGCCG CGTTAAGACC GCGGGTCCGA
     3361 GCACTCCGGT TGAGATTACG GGTCTGAACG ATGTTCCGCA AGCCGGTGAC CAATTTCTGG
     3421 TTTTCAAAGA TGAAAAAACG GCTCGTAGCG TCGGTGAGGC GCGCGCGTCT AAACAACTGG
     3481 AGGAGCAGCG CAGCGATAAG GCCAAACTGA GCCTGGACGA CCTGTTCGAG CAGATTAAAC
     3541 AGGGTGACGT CAAGGACATC AACCTGATTG TCAAAGCAGA TGTTCAGGGT TCTGCCGAGG
     3601 CGCTGACGGC TGCGCTGCAG AAGATTGAAG TGGAAGGTGT TAAAGTTAAG ATCATCCACA
     3661 CTGGTGTTGG CGCGATTACT GAGTCTGATA TCATTCTGGC ATCTGCCTCC AACGCGATTG
     3721 TGATCGGCTT CAACGTTCGC CCGGATGGCA ACGCAAAGAG CACCGCAGAA GCGGAGAACG
     3781 TCGATATCCG TCTGCACCGT ATTATCTACA AAGTCATTGA CGAGATTGAG GCAGCAATGA
     3841 AGGGCATGCT GGACCCGGAA TACGAGGAGA AGGTTATCGG TCAAGTGGAA GTTCGTCAGA
     3901 CGTTTAAGGT GAGCAAGATC GGTACTATCG CGGGCGGTTA CGTCACCGAG GGTACGATTA
     3961 CGCGTGATTC TGGCCTGCGT CTGATTCGTG ATGGTGTTGT TATCTTTGAG GGCGAAGTCG
     4021 ACGTCTTGAA GCGCTTCAAA GATGACGTGA AGGAAGTTAG CCAAGGCTAT GAGTGCGGCA
     4081 TTACCATTAA GAAATACAAT GACATTAGAG AGGGTGACAT TCTGGAGGCG TTCGTGATGC
     4141 AAGAGATTGA GCGCACCTGA AGAGCTTAGA GACC
//