LOCUS Exported 3583 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3583 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1621..1668 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3516..3532 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3533..3564 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3582 /label="ymdA" /note="Endoribonuclease that initiates mRNA decay. Initiates the decay of all SAM-dependent riboswitches, such as yitJ riboswitch. Involved in processing of the gapA operon mRNA, it cleaves between cggR and gapA (PubMed:19193632). Is also the decay-initiating endonuclease for rpsO mRNA. Involved in degradation of type I toxin-antitoxin system bsrG/SR4 RNAs and a minor role in degradation of type I toxin-antitoxin system bsrE/SR5 degradation (PubMed:22229825, PubMed:26940229)." /translation="MTPIMMVLISILLILLGLVVGYFVRKTIAEAKIAGARGAAEQILEDAKRDAEALKKEALLEAKDEIHKLRIDAEQEVRERRNELQKQENRLLQKEENLDRKHEGIDKREAMLEKKDHSLNERQQHIEEMESKVDEMIRMQQSELERISSLTRDEAKQIILERVENELSHDIAIMTKETENRAKEEADKKAKNILSLALQRCAADHVAETTVSVVNLPNDEMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRRETARIALDKLVQDGRIHPARIEEMVEKSRREVDDYIREMGEQTTFEVGVHGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAFLAGLMASELGEDAKLAKRAGLLHDIGKAIDHEVEGSHVEIGVELATKYKEHPVVINSIASHHGDEEPTSIIAVLVAAADALSAARPGARSETLENYIRRLEKLEEISESYEGVEKSFAIQAGREVRIMVKPDSINDLEAHRLARDIRKRIEDELDYPGHIKVTVIRETRAVEYAK" primer_bind complement(1673..1689) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1756..2344) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2535..3395) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3396..3500) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 GTCTCAAATG ACCCCGATTA TGATGGTTCT GATTTCGATT CTGTTGATTC TGCTGGGTCT 61 GGTTGTCGGT TATTTCGTTC GCAAGACGAT TGCCGAGGCG AAAATTGCCG GCGCACGTGG 121 TGCCGCGGAG CAAATCCTGG AGGACGCCAA GCGCGACGCG GAGGCCCTGA AGAAGGAAGC 181 CCTGCTGGAG GCAAAGGACG AAATCCACAA ACTGCGTATT GACGCCGAAC AGGAGGTGCG 241 TGAGCGCCGT AACGAACTGC AAAAGCAGGA AAACCGTTTG CTGCAAAAAG AAGAGAATCT 301 GGATCGTAAA CACGAGGGTA TCGATAAGCG TGAAGCCATG CTGGAGAAAA AGGACCACAG 361 CCTGAATGAG CGTCAGCAAC ATATTGAGGA GATGGAGAGC AAGGTCGACG AAATGATCCG 421 TATGCAACAG AGCGAATTGG AGCGCATTAG CTCGCTGACC CGCGACGAAG CAAAGCAGAT 481 TATTCTGGAG CGTGTGGAAA ATGAGCTGAG CCACGATATC GCGATTATGA CTAAGGAAAC 541 CGAGAATCGT GCGAAGGAAG AGGCCGACAA AAAAGCGAAG AACATTCTGT CTCTGGCTCT 601 GCAACGTTGC GCGGCGGACC ATGTGGCCGA GACTACCGTC AGCGTGGTCA ATCTGCCGAA 661 TGACGAAATG AAGGGCCGTA TCATCGGTCG TGAAGGTCGT AATATCCGTA CCCTGGAAAC 721 GCTGACCGGT ATTGATCTGA TTATTGATGA CACGCCGGAG GCTGTTATCT TGAGCGGCTT 781 CGATCCGATT CGTCGCGAAA CCGCGCGCAT TGCTCTGGAC AAGCTGGTCC AGGACGGTCG 841 CATCCATCCG GCACGTATTG AGGAGATGGT TGAAAAATCT CGTCGCGAAG TGGATGATTA 901 CATTCGTGAG ATGGGTGAGC AGACCACCTT TGAGGTAGGC GTGCACGGTC TGCATCCGGA 961 TCTGATTAAG ATCCTGGGTC GTTTGAAGTT CCGTACTTCT TATGGTCAGA ACGTTCTGAA 1021 GCACAGCATG GAGGTGGCCT TCTTGGCGGG TCTGATGGCG AGCGAGCTGG GTGAAGATGC 1081 GAAACTGGCA AAACGTGCGG GCCTGTTGCA CGACATTGGT AAGGCTATCG ATCACGAAGT 1141 TGAAGGTAGC CATGTGGAGA TCGGCGTCGA GTTGGCGACT AAATACAAGG AACACCCAGT 1201 CGTGATCAAC AGCATTGCGA GCCACCATGG TGACGAGGAA CCGACCTCCA TTATTGCAGT 1261 CCTGGTCGCG GCAGCGGACG CTTTATCAGC CGCTCGTCCA GGCGCCCGCA GCGAAACCCT 1321 GGAGAATTAC ATCCGTCGCC TGGAAAAGCT GGAGGAAATC AGCGAGAGCT ATGAGGGCGT 1381 GGAAAAGAGC TTTGCAATTC AGGCCGGTCG TGAAGTTCGT ATCATGGTTA AACCAGACTC 1441 TATCAATGAC CTGGAAGCAC ACCGCCTGGC GCGTGATATC CGTAAGCGCA TCGAGGATGA 1501 GCTGGATTAC CCTGGCCATA TCAAGGTTAC CGTCATCCGC GAAACTCGTG CCGTCGAGTA 1561 TGCGAAGTGA AGAGCTTAGA GACCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA 1621 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC 1681 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA 1741 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 1801 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 1861 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 1921 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 1981 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 2041 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 2101 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 2161 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 2221 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 2281 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 2341 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 2401 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 2461 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 2521 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 2581 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 2641 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT 2701 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 2761 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 2821 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 2881 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 2941 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 3001 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 3061 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 3121 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 3181 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 3241 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 3301 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 3361 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 3421 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 3481 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA 3541 GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGG GAG //