LOCUS       Exported     2998 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2998
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1036..1083
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2931..2947
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2948..2979
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..2997
                     /label="birA"
                     /note="Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor."
                     /translation="MRSTLRKDLIELFSQAGNEFISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTEVMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGVWMSLILRPDIPLQKTPQLTLLAAVAVVQGIEEAAGIQTDIKWPNDILINGKKTVGILTEMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEKIDRAGVIQHILLCFEKRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNGTFYGKALGIDDEGVLLLETNEGIKKIYSADIELG"
     primer_bind     complement(1088..1104)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1171..1759)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1950..2810)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2811..2915)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG CGTTCCACCC TGCGTAAGGA TCTGATCGAG CTGTTCAGCC AGGCCGGCAA
       61 CGAGTTTATT AGCGGTCAAA AGATCTCGGA CGCGCTGGGC TGCAGCCGCA CCGCAGTATG
      121 GAAACATATT GAGGAACTCC GCAAAGAGGG TTATGAAGTG GAAGCAGTTC GCCGCAAAGG
      181 CTACCGTCTG ATTAAGAAGC CTGGTAAACT GAGCGAGAGC GAAATTCGTT TTGGCCTCAA
      241 GACCGAGGTC ATGGGCCAGC ACCTGATCTA TCACGATGTG CTGAGCAGCA CGCAGAAAAC
      301 CGCCCACGAG CTTGCCAATA ACAATGCACC AGAGGGTACG CTTGTTGTGG CAGACAAGCA
      361 AACCGCCGGT CGCGGTCGTA TGAGCCGTGT GTGGCACTCA CAAGAGGGCA ATGGTGTCTG
      421 GATGTCCCTG ATCCTGCGTC CAGATATCCC TCTGCAAAAA ACGCCGCAAC TGACGCTGCT
      481 GGCCGCTGTC GCAGTTGTGC AGGGTATCGA AGAGGCGGCG GGCATTCAGA CCGACATCAA
      541 GTGGCCGAAC GATATTCTGA TCAACGGCAA AAAGACCGTC GGTATTCTGA CCGAAATGCA
      601 GGCAGAGGAG GATCGTGTTC GTAGCGTGAT TATCGGTATC GGCATCAACG TGAACCAGCA
      661 GCCGAATGAC TTTCCTGATG AACTGAAGGA CATTGCAACC AGCTTGAGCC AGGCAGCTGG
      721 TGAAAAGATC GATCGTGCCG GTGTTATCCA GCATATCCTG CTGTGCTTCG AGAAACGCTA
      781 TCGTGATTAT ATGACGCACG GCTTCACCCC GATTAAGTTG CTGTGGGAAT CCTATGCCCT
      841 GGGTATTGGT ACGAACATGC GTGCCCGTAC CCTGAACGGT ACCTTCTACG GTAAAGCACT
      901 GGGTATTGAC GACGAGGGTG TTTTGCTGCT GGAAACCAAT GAGGGTATCA AGAAGATCTA
      961 CAGCGCAGAC ATCGAGCTGG GCTGAAGAGC TTAGAGACCG CTGAGGTGTC AATCGTCGGA
     1021 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1081 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1141 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1201 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1261 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1321 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1381 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1441 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1501 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1561 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1621 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1681 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1741 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1801 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1861 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1921 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1981 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2041 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2101 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2161 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2221 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2281 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2341 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2401 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2461 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2521 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2581 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2641 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2701 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2761 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2821 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2881 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2941 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//