LOCUS       Exported     3307 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3307
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1345..1392
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3240..3256
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3257..3288
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3306
                     /label="aroE"
                     /note="Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate."
                     /translation="MKRDKVQTLHGEIHIPGDKSISHRSVMFGALAAGTTTVKNFLPGADCLSTIDCFRKMGVHIEQSSSDVVIHGKGIDALKEPESLLDVGNSGTTIRLMLGILAGRPFYSAVAGDESIAKRPMKRVTEPLKKMGAKIDGRAGGEFTPLSVSGASLKGIDYVSPVASAQIKSAVLLAGLQAEGTTTVTEPHKSRDHTERMLSAFGVKLSEDQTSVSIAGGQKLTAADIFVPGDISSAAFFLAAGAMVPNSRIVLKNVGLNPTRTGIIDVLQNMGAKLEIKPSADSGAEPYGDLIIETSSLKAVEIGGDIIPRLIDEIPIIALLATQAEGTTVIKDAAELKVKETNRIDTVVSELRKLGAEIEPTADGMKVYGKQTLKGGAAVSSHGDHRIGMMLGIASCITEEPIEIEHTDAIHVSYPTFFEHLNKLSKKS"
     primer_bind     complement(1397..1413)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1480..2068)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2259..3119)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3120..3224)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG AAGCGTGATA AGGTCCAGAC TTTACACGGT GAGATTCATA TTCCAGGCGA
       61 CAAATCGATT AGCCACCGTA GCGTCATGTT TGGCGCACTG GCAGCTGGCA CTACCACCGT
      121 GAAAAACTTC TTGCCGGGTG CGGACTGCCT GTCAACCATT GACTGCTTTC GTAAAATGGG
      181 CGTGCATATT GAACAAAGCA GCTCGGATGT GGTGATCCAT GGCAAAGGTA TCGACGCACT
      241 GAAAGAGCCG GAAAGCCTGC TGGACGTGGG TAATAGCGGT ACCACCATCC GTCTGATGTT
      301 GGGCATCCTG GCCGGCCGCC CGTTTTACAG CGCAGTGGCG GGCGACGAAA GCATTGCAAA
      361 AAGACCGATG AAGCGCGTCA CCGAACCGCT GAAGAAAATG GGTGCAAAAA TTGACGGCCG
      421 TGCGGGTGGC GAGTTCACGC CGCTCAGCGT TTCCGGTGCG TCCTTGAAGG GTATCGACTA
      481 TGTTAGCCCG GTCGCTTCCG CCCAAATTAA GAGCGCCGTA CTGTTGGCGG GTCTGCAAGC
      541 GGAGGGTACC ACTACCGTTA CCGAGCCGCA TAAGTCTCGC GACCACACGG AACGTATGCT
      601 GTCTGCATTC GGCGTGAAGC TGTCCGAGGA TCAAACGTCG GTTTCGATCG CGGGTGGTCA
      661 GAAGCTGACG GCGGCGGACA TCTTCGTGCC GGGCGACATC AGCAGCGCGG CGTTCTTCCT
      721 CGCCGCGGGT GCTATGGTGC CGAATAGCCG TATTGTGCTG AAAAACGTCG GTCTGAATCC
      781 GACCCGCACC GGCATTATCG ACGTGCTGCA AAACATGGGT GCGAAGCTGG AGATCAAACC
      841 GAGCGCGGAC TCTGGCGCGG AGCCATATGG CGACCTGATT ATTGAAACGA GCAGCTTGAA
      901 AGCGGTTGAG ATCGGTGGTG ACATTATCCC GCGTTTGATT GATGAGATTC CGATCATTGC
      961 GCTGCTGGCC ACGCAAGCGG AGGGTACCAC CGTGATTAAG GACGCGGCGG AGTTGAAGGT
     1021 TAAGGAAACC AACCGTATTG ATACGGTGGT GTCCGAGCTG CGCAAGCTCG GTGCCGAGAT
     1081 CGAACCGACC GCTGACGGCA TGAAGGTGTA CGGTAAGCAG ACCTTGAAGG GTGGTGCTGC
     1141 AGTTAGCAGC CATGGTGACC ACCGCATCGG CATGATGCTG GGCATTGCGT CCTGTATTAC
     1201 CGAGGAACCG ATCGAAATTG AACACACCGA TGCAATCCAT GTGAGCTATC CGACCTTTTT
     1261 CGAACATCTG AATAAACTGA GCAAAAAGTC CTGAAGAGCT TAGAGACCGC TGAGGTGTCA
     1321 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1381 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1441 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1501 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1561 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1621 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1681 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1741 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1801 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1861 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1921 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1981 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     2041 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     2101 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     2161 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     2221 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     2281 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2341 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     2401 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     2461 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     2521 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     2581 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2641 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     2701 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     2761 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     2821 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     2881 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     2941 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     3001 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     3061 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     3121 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     3181 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     3241 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA
     3301 GTGGGAG
//