LOCUS Exported 3067 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3067 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1105..1152 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3000..3016 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3017..3048 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3066 /label="hepT" /note="Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7)." /translation="MLNIIRLLAESLPRISDGNENTDVWVNDMKFKMAYSFLNDDIDVIERELEQTVRSDYPLLSEAGLHLLQAGGKRIRPVFVLLSGMFGDYDINKIKYVAVTLEMIHMASLVHDDVIDDAELRRGKPTIKAKWDNRIAMYTGDYMLAGSLEMMTRINEPKAHRILSQTIVEVCLGEIEQIKDKYNMEQNLRTYLRRIKRKTALLIAVSCQLGAIASGADEKIHKALYWFGYYVGMSYQIIDDILDFTSTEEELGKPVGGDLLQGNVTLPVLYALKNPALKNQLKLINSETTQEQLEPIIEEIKKTDAIEASMAVSEMYLQKAFQKLNTLPRGRARSSLAAIAKYIGKRKF" primer_bind complement(1157..1173) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1240..1828) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2019..2879) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2880..2984) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 GTCTCAAATG TTGAATATTA TTCGTCTGCT GGCTGAAAGC CTGCCGCGCA TTTCGGATGG 61 TAATGAAAAT ACGGACGTCT GGGTGAATGA TATGAAATTC AAAATGGCGT ACAGCTTTCT 121 GAATGATGAT ATTGATGTCA TTGAGCGTGA GCTGGAGCAG ACCGTTCGCT CAGATTACCC 181 GCTGCTGAGC GAAGCGGGCC TGCATCTGCT CCAGGCGGGT GGTAAGCGCA TTCGTCCGGT 241 CTTTGTGCTG TTGAGCGGTA TGTTTGGTGA TTACGACATC AATAAGATCA AGTACGTTGC 301 AGTTACCCTG GAAATGATCC ATATGGCCTC CCTGGTTCAT GACGACGTCA TCGACGATGC 361 AGAACTGCGT CGCGGTAAAC CGACGATCAA AGCCAAATGG GACAACCGTA TCGCAATGTA 421 CACCGGTGAT TACATGTTGG CTGGTTCTCT GGAGATGATG ACTCGTATCA ATGAGCCGAA 481 AGCGCACCGC ATTCTGTCAC AAACCATCGT GGAAGTTTGC CTGGGCGAAA TCGAACAGAT 541 TAAAGACAAG TATAACATGG AGCAGAACTT GCGTACGTAT CTGCGCCGTA TTAAGCGCAA 601 GACCGCATTG CTGATTGCGG TGAGCTGCCA GCTGGGTGCA ATCGCGTCAG GTGCAGACGA 661 GAAAATTCAT AAAGCACTTT ATTGGTTCGG CTACTACGTG GGCATGTCCT ACCAAATTAT 721 CGATGACATC TTAGACTTTA CCAGCACCGA GGAGGAATTG GGTAAACCGG TCGGTGGCGA 781 CTTGCTGCAA GGCAACGTGA CGCTGCCGGT TTTGTACGCG CTGAAAAATC CGGCTCTGAA 841 GAATCAGTTG AAGCTGATTA ACAGCGAAAC CACTCAAGAG CAGCTGGAAC CGATCATCGA 901 GGAGATCAAG AAAACCGACG CGATTGAGGC CAGCATGGCG GTTAGCGAGA TGTATCTGCA 961 AAAAGCTTTC CAAAAGCTGA ATACCCTGCC GCGTGGCCGT GCCCGTTCGA GCTTGGCAGC 1021 AATCGCGAAA TATATCGGTA AACGTAAATT TTGAAGAGCT TAGAGACCGC TGAGGTGTCA 1081 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG 1141 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT 1201 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC 1261 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 1321 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 1381 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 1441 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 1501 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 1561 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 1621 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 1681 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 1741 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 1801 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG 1861 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 1921 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 1981 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 2041 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 2101 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 2161 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 2221 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 2281 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC 2341 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA 2401 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG 2461 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT 2521 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC 2581 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG 2641 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG 2701 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT 2761 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA 2821 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA 2881 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 2941 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG 3001 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA 3061 GTGGGAG //