LOCUS       Exported     3196 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3196
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1234..1281
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3129..3145
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3146..3177
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3195
                     /label="resC"
                     /note="Required for the biogenesis of c-type cytochromes."
                     /translation="MAELSGNFLYAAFLVYLIAVPIFGGAIRGNKDKKGRPNRWANIGITLSIVGFFCHLGYFITRWAASGHAPVSNMFEFTTAFGMMLVLAFIILYFLYRLPSLGLFTLSIALLLIAYASMFPTDISPLIPSLQSNWLYIHVTTAALGQAILAISFVAGVIFLLKHVDQTKPSKKTFWLEAIMFILVTTVAFIAITSAFRLAGYEAEFNWVDKSKEKSVMVYEMPALVGPHQGELLTEGRMEPLVDLPALFSGRKVNTVIWSFGAGLVLYGALRLIIRKRISALLHPLVKNVNLDLVDEVGYRAVSIGFPIFTLGALIFAMIWAQLAWTRFWGWDPKEVWALITFLFYAAYLHLRLSRGWHGEKSAWLAVIGFAIIMFNLIFVNLVLAGLHSYA"
     primer_bind     complement(1286..1302)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1369..1957)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2148..3008)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3009..3113)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG GCAGAACTGT CCGGTAATTT CCTGTATGCG GCGTTCCTGG TCTATCTGAT
       61 CGCGGTTCCG ATTTTTGGTG GTGCTATTCG TGGTAATAAA GACAAAAAAG GCCGTCCGAA
      121 CCGCTGGGCG AACATTGGCA TTACCCTGAG CATCGTCGGC TTCTTCTGTC ACCTGGGTTA
      181 CTTTATTACC CGTTGGGCGG CGTCAGGTCA TGCGCCGGTG AGCAACATGT TCGAGTTTAC
      241 CACTGCATTT GGTATGATGC TGGTCCTGGC GTTTATTATC CTGTATTTCC TGTACCGCCT
      301 GCCAAGCCTG GGTCTGTTCA CGCTGTCTAT CGCACTGCTG TTAATTGCGT ATGCGTCTAT
      361 GTTTCCGACC GATATCAGCC CGCTGATTCC TAGCCTGCAA AGCAATTGGC TGTATATCCA
      421 CGTCACGACG GCAGCCCTGG GTCAAGCGAT TCTGGCCATT AGCTTCGTGG CCGGTGTTAT
      481 CTTTTTGTTG AAGCATGTGG ACCAGACGAA GCCGTCGAAG AAAACCTTTT GGCTGGAGGC
      541 GATTATGTTT ATCTTGGTTA CGACTGTTGC ATTCATTGCG ATTACCAGCG CATTCCGTCT
      601 GGCGGGTTAC GAGGCAGAGT TTAACTGGGT CGACAAATCT AAAGAAAAGA GCGTGATGGT
      661 GTACGAGATG CCGGCGCTGG TTGGTCCGCA CCAAGGCGAG CTGCTGACCG AAGGCCGTAT
      721 GGAACCTTTA GTCGATTTGC CGGCCCTGTT CAGCGGTCGT AAGGTGAACA CCGTGATTTG
      781 GAGCTTTGGT GCCGGTCTGG TCCTGTATGG TGCTCTGCGC CTCATCATTC GTAAGCGCAT
      841 CAGCGCACTG CTGCACCCGC TGGTGAAAAA CGTGAACCTG GATTTGGTCG ATGAGGTTGG
      901 CTATCGCGCT GTCAGCATCG GTTTCCCGAT CTTTACCCTG GGTGCCCTGA TTTTCGCAAT
      961 GATCTGGGCT CAGCTGGCCT GGACGCGTTT TTGGGGTTGG GACCCGAAGG AGGTTTGGGC
     1021 GCTGATCACC TTCCTGTTTT ACGCGGCCTA TCTGCATCTG CGCTTGTCGC GTGGCTGGCA
     1081 CGGCGAGAAA TCCGCATGGC TGGCAGTGAT TGGCTTCGCA ATCATTATGT TCAACCTGAT
     1141 CTTTGTGAAC TTGGTGTTGG CCGGTTTGCA TTCCTACGCC TGAAGAGCTT AGAGACCGCT
     1201 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1261 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1321 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1381 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1441 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1501 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1561 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1621 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1681 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1741 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1801 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1861 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1921 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1981 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2041 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2101 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2161 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2221 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2281 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2341 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2401 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2461 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2521 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2581 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2641 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2701 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2761 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2821 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2881 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2941 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3001 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3061 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3121 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3181 TCAGGTGGAG TGGGAG
//