LOCUS       Exported     3925 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3925
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3911
                     /label="dxs"
                     /note="Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)."
                     /translation="MDLLSIQDPSFLKNMSIDELEKLSDEIRQFLITSLSASGGHIGPNLGVVELTVALHKEFNSPKDKFLWDVGHQSYVHKLLTGRGKEFATLRQYKGLCGFPKRSESEHDVWETGHSSTSLSGAMGMAAARDIKGTDEYIIPIIGDGALTGGMALEALNHIGDEKKDMIVILNDNEMSIAPNVGAIHSMLGRLRTAGKYQWVKDELEYLFKKIPAVGGKLAATAERVKDSLKYMLVSGMFFEELGFTYLGPVDGHSYHELIENLQYAKKTKGPVLLHVITKKGKGYKPAETDTIGTWHGTGPYKINTGDFVKPKAAAPSWSGLVSGTVQRMAREDGRIVAITPAMPVGSKLEGFAKEFPDRMFDVGIAEQHAATMAAAMAMQGMKPFLAIYSTFLQRAYDQVVHDICRQNANVFIGIDRAGLVGADGETHQGVFDIAFMRHIPNMVLMMPKDENEGQHMVHTALSYDEGPIAMRFPRGNGLGVKMDEQLKTIPIGTWEVLRPGNDAVILTFGTTIEMAIEAAEELQKEGLSVRVVNARFIKPIDEKMMKSILKEGLPILTIEEAVLEGGFGSSILEFAHDQGEYHTPIDRMGIPDRFIEHGSVTALLEEIGLTKQQVANRIRLLMPPKTHKGIGS"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGATCTGCT GAGCATCCAG GATCCGTCTT
     2041 TCCTGAAGAA CATGTCCATC GATGAACTGG AGAAACTGTC GGACGAAATC CGTCAGTTTT
     2101 TGATTACCAG CCTGAGCGCA AGCGGCGGCC ATATCGGTCC GAATCTGGGT GTGGTGGAGT
     2161 TGACTGTTGC CTTGCACAAA GAGTTTAATT CCCCGAAAGA CAAATTTCTG TGGGACGTTG
     2221 GTCATCAGAG CTATGTGCAC AAGCTGCTGA CCGGCCGCGG CAAAGAATTC GCAACTCTGC
     2281 GCCAGTATAA GGGCCTGTGC GGTTTTCCGA AGCGTTCTGA GTCCGAGCAC GACGTATGGG
     2341 AAACCGGTCA CTCCTCTACC TCCCTGAGCG GCGCCATGGG TATGGCGGCG GCGCGTGATA
     2401 TCAAGGGCAC CGACGAATAC ATCATTCCTA TCATTGGTGA TGGTGCTCTG ACGGGTGGTA
     2461 TGGCGCTGGA GGCGCTGAAC CATATTGGCG ACGAGAAGAA AGATATGATT GTGATCCTTA
     2521 ATGACAATGA AATGTCAATC GCGCCGAATG TCGGTGCGAT TCATAGCATG CTGGGTCGTC
     2581 TGCGCACCGC GGGTAAGTAC CAGTGGGTGA AGGATGAACT GGAGTACCTG TTTAAGAAAA
     2641 TTCCGGCCGT CGGCGGCAAG TTGGCGGCCA CGGCGGAGCG TGTGAAGGAC AGCTTGAAGT
     2701 ACATGCTGGT TAGCGGTATG TTTTTCGAAG AATTGGGCTT TACGTACCTG GGTCCGGTGG
     2761 ATGGCCATAG CTACCACGAA TTGATTGAGA ATCTGCAATA CGCGAAAAAG ACCAAAGGTC
     2821 CGGTGCTGTT GCATGTCATT ACCAAAAAGG GCAAGGGCTA CAAGCCGGCC GAAACCGATA
     2881 CCATTGGCAC CTGGCACGGT ACGGGCCCGT ACAAAATCAA CACCGGTGAC TTTGTGAAAC
     2941 CGAAGGCGGC GGCGCCAAGC TGGAGCGGTC TGGTTAGCGG CACCGTCCAG CGTATGGCAC
     3001 GTGAGGACGG TCGTATTGTT GCGATCACCC CGGCCATGCC GGTGGGTTCA AAATTAGAGG
     3061 GTTTCGCGAA GGAATTCCCG GACCGCATGT TCGATGTGGG TATCGCTGAA CAACACGCCG
     3121 CCACCATGGC GGCGGCAATG GCCATGCAGG GCATGAAGCC GTTCCTGGCA ATCTATTCTA
     3181 CGTTCCTGCA GCGTGCTTAC GATCAGGTGG TTCATGATAT TTGCCGTCAG AATGCGAATG
     3241 TTTTCATTGG TATCGATCGT GCAGGCCTGG TGGGTGCGGA TGGCGAAACC CACCAGGGTG
     3301 TCTTTGATAT CGCATTCATG AGACACATCC CGAACATGGT TCTGATGATG CCTAAGGACG
     3361 AGAATGAGGG CCAACACATG GTCCATACGG CGCTGAGCTA CGACGAAGGC CCGATCGCCA
     3421 TGCGCTTTCC GCGTGGTAAT GGCCTGGGCG TTAAGATGGA TGAACAGTTA AAGACCATCC
     3481 CGATTGGCAC GTGGGAGGTG CTGCGTCCGG GTAACGATGC GGTTATCCTG ACCTTCGGCA
     3541 CCACGATCGA GATGGCAATT GAGGCGGCAG AGGAGCTGCA GAAGGAGGGC CTGTCGGTCC
     3601 GCGTCGTTAA TGCGCGCTTC ATTAAGCCGA TTGATGAGAA AATGATGAAA AGCATCCTGA
     3661 AGGAGGGCCT GCCGATCCTG ACCATTGAGG AAGCGGTTTT GGAGGGTGGC TTCGGTTCTA
     3721 GCATTCTGGA GTTCGCTCAC GATCAGGGTG AATACCACAC CCCGATTGAT CGTATGGGTA
     3781 TCCCGGACCG TTTCATCGAG CACGGTAGCG TGACGGCTCT GCTGGAAGAG ATTGGCCTGA
     3841 CCAAGCAACA GGTGGCCAAT CGTATCCGTT TGCTGATGCC GCCGAAAACC CACAAAGGCA
     3901 TTGGCTCCTG AAGAGCTTAG AGACC
//