LOCUS       Exported     3373 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3373
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1411..1458
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3306..3322
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3323..3354
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3372
                     /label="accC"
                     /note="This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA."
                     /translation="MIKKLLIANRGEIAVRIIRACRELGIETVAVYSEADKDALHVQMADEAFCIGPKASKDSYLNVTNIVSVAKLTGTDAIHPGYGFLAENADFAELCEEVNVTFVGPSADAISKMGTKDVARETMKQAGVPIVPGSQGIIENVEEAVSLANEIGYPVIIKATAGGGGKGIRVARTEEELINGIKITQQEAATAFGNPGVYIEKYIEDFRHVEIQVLADNYGNTIHLGERDCSIQRRLQKLLEESPSPALDSEIREQMGDAAVKAAKAVGYTGAGTVEFIYDYNEQRYYFMEMNTRIQVEHPVTEMVTGTDLIKEQIKVASGMELSLKQEDVEFEGWAIECRINAENPSKNFMPSPGEIKMYLPPGGLGVRVDSAAYPGYSIPPYYDSMIAKVITYGKTRDEAIARMKRALSEFVIEGIETTIPFHLKLLEHETFVSGEFNTKFLETYDVMGS"
     primer_bind     complement(1463..1479)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1546..2134)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2325..3185)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3186..3290)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG ATTAAGAAAC TGCTGATCGC AAATCGCGGT GAGATTGCGG TTCGCATTAT
       61 CCGTGCGTGC CGCGAACTGG GCATCGAAAC CGTGGCGGTC TATAGCGAGG CGGATAAGGA
      121 CGCGCTGCAC GTCCAGATGG CCGATGAAGC TTTCTGTATC GGTCCGAAAG CGAGCAAGGA
      181 CTCCTACTTG AACGTGACTA ATATTGTGAG CGTTGCAAAG TTGACCGGTA CCGACGCGAT
      241 TCACCCGGGT TACGGTTTCC TGGCGGAAAA CGCGGACTTT GCAGAACTGT GCGAAGAGGT
      301 TAACGTTACG TTTGTTGGTC CGAGCGCCGA CGCCATTAGC AAAATGGGTA CCAAAGACGT
      361 TGCTCGTGAA ACGATGAAGC AGGCGGGCGT GCCGATCGTG CCGGGTAGCC AGGGTATTAT
      421 CGAGAATGTG GAGGAGGCGG TGAGCCTGGC GAACGAGATT GGCTATCCGG TTATCATCAA
      481 GGCAACCGCG GGCGGTGGCG GCAAGGGTAT CCGCGTTGCC CGTACGGAAG AAGAACTGAT
      541 CAACGGCATC AAGATCACCC AACAAGAGGC CGCAACCGCG TTTGGTAATC CAGGTGTTTA
      601 CATTGAGAAG TATATCGAAG ATTTCCGTCA CGTTGAAATT CAAGTCCTGG CAGACAATTA
      661 TGGCAATACG ATTCACCTGG GCGAACGTGA TTGCAGCATT CAGCGCCGTC TGCAGAAGCT
      721 GTTGGAAGAG AGCCCGAGCC CGGCGCTGGA CTCCGAGATT CGTGAACAGA TGGGCGACGC
      781 AGCCGTGAAA GCGGCCAAAG CGGTCGGTTA CACCGGTGCG GGCACCGTGG AGTTCATCTA
      841 TGACTACAAT GAGCAGCGTT ATTACTTTAT GGAGATGAAC ACGCGTATCC AGGTTGAGCA
      901 TCCTGTCACT GAGATGGTTA CTGGTACCGA CCTTATTAAG GAACAGATCA AAGTGGCCAG
      961 CGGCATGGAA CTGTCTCTGA AGCAGGAGGA CGTTGAGTTC GAGGGTTGGG CTATCGAGTG
     1021 CCGCATTAAC GCGGAAAACC CATCCAAGAA CTTCATGCCG AGCCCGGGTG AAATCAAAAT
     1081 GTATCTGCCG CCGGGTGGTC TGGGTGTTCG TGTAGACAGC GCTGCGTACC CAGGCTATAG
     1141 CATCCCGCCG TATTATGACA GCATGATCGC CAAAGTGATC ACCTACGGTA AGACCCGCGA
     1201 CGAGGCGATT GCACGCATGA AACGCGCCTT GAGCGAGTTC GTTATCGAAG GTATCGAAAC
     1261 TACCATTCCG TTTCACTTGA AACTGCTGGA GCACGAAACC TTTGTGAGCG GCGAATTCAA
     1321 CACCAAATTT CTGGAGACTT ATGATGTTAT GGGTTCCTGA AGAGCTTAGA GACCGCTGAG
     1381 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1441 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1501 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1561 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1621 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1681 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1741 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1801 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1861 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1921 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1981 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2041 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2101 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2161 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2221 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2281 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2341 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2401 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2461 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2521 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2581 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2641 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2701 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2761 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2821 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2881 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2941 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     3001 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     3061 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3121 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3181 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3241 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3301 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     3361 GGTGGAGTGG GAG
//