LOCUS Exported 3373 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3373 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1411..1458 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3306..3322 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3323..3354 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3372 /label="accC" /note="This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA." /translation="MIKKLLIANRGEIAVRIIRACRELGIETVAVYSEADKDALHVQMADEAFCIGPKASKDSYLNVTNIVSVAKLTGTDAIHPGYGFLAENADFAELCEEVNVTFVGPSADAISKMGTKDVARETMKQAGVPIVPGSQGIIENVEEAVSLANEIGYPVIIKATAGGGGKGIRVARTEEELINGIKITQQEAATAFGNPGVYIEKYIEDFRHVEIQVLADNYGNTIHLGERDCSIQRRLQKLLEESPSPALDSEIREQMGDAAVKAAKAVGYTGAGTVEFIYDYNEQRYYFMEMNTRIQVEHPVTEMVTGTDLIKEQIKVASGMELSLKQEDVEFEGWAIECRINAENPSKNFMPSPGEIKMYLPPGGLGVRVDSAAYPGYSIPPYYDSMIAKVITYGKTRDEAIARMKRALSEFVIEGIETTIPFHLKLLEHETFVSGEFNTKFLETYDVMGS" primer_bind complement(1463..1479) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1546..2134) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2325..3185) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(3186..3290) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 GTCTCAAATG ATTAAGAAAC TGCTGATCGC AAATCGCGGT GAGATTGCGG TTCGCATTAT 61 CCGTGCGTGC CGCGAACTGG GCATCGAAAC CGTGGCGGTC TATAGCGAGG CGGATAAGGA 121 CGCGCTGCAC GTCCAGATGG CCGATGAAGC TTTCTGTATC GGTCCGAAAG CGAGCAAGGA 181 CTCCTACTTG AACGTGACTA ATATTGTGAG CGTTGCAAAG TTGACCGGTA CCGACGCGAT 241 TCACCCGGGT TACGGTTTCC TGGCGGAAAA CGCGGACTTT GCAGAACTGT GCGAAGAGGT 301 TAACGTTACG TTTGTTGGTC CGAGCGCCGA CGCCATTAGC AAAATGGGTA CCAAAGACGT 361 TGCTCGTGAA ACGATGAAGC AGGCGGGCGT GCCGATCGTG CCGGGTAGCC AGGGTATTAT 421 CGAGAATGTG GAGGAGGCGG TGAGCCTGGC GAACGAGATT GGCTATCCGG TTATCATCAA 481 GGCAACCGCG GGCGGTGGCG GCAAGGGTAT CCGCGTTGCC CGTACGGAAG AAGAACTGAT 541 CAACGGCATC AAGATCACCC AACAAGAGGC CGCAACCGCG TTTGGTAATC CAGGTGTTTA 601 CATTGAGAAG TATATCGAAG ATTTCCGTCA CGTTGAAATT CAAGTCCTGG CAGACAATTA 661 TGGCAATACG ATTCACCTGG GCGAACGTGA TTGCAGCATT CAGCGCCGTC TGCAGAAGCT 721 GTTGGAAGAG AGCCCGAGCC CGGCGCTGGA CTCCGAGATT CGTGAACAGA TGGGCGACGC 781 AGCCGTGAAA GCGGCCAAAG CGGTCGGTTA CACCGGTGCG GGCACCGTGG AGTTCATCTA 841 TGACTACAAT GAGCAGCGTT ATTACTTTAT GGAGATGAAC ACGCGTATCC AGGTTGAGCA 901 TCCTGTCACT GAGATGGTTA CTGGTACCGA CCTTATTAAG GAACAGATCA AAGTGGCCAG 961 CGGCATGGAA CTGTCTCTGA AGCAGGAGGA CGTTGAGTTC GAGGGTTGGG CTATCGAGTG 1021 CCGCATTAAC GCGGAAAACC CATCCAAGAA CTTCATGCCG AGCCCGGGTG AAATCAAAAT 1081 GTATCTGCCG CCGGGTGGTC TGGGTGTTCG TGTAGACAGC GCTGCGTACC CAGGCTATAG 1141 CATCCCGCCG TATTATGACA GCATGATCGC CAAAGTGATC ACCTACGGTA AGACCCGCGA 1201 CGAGGCGATT GCACGCATGA AACGCGCCTT GAGCGAGTTC GTTATCGAAG GTATCGAAAC 1261 TACCATTCCG TTTCACTTGA AACTGCTGGA GCACGAAACC TTTGTGAGCG GCGAATTCAA 1321 CACCAAATTT CTGGAGACTT ATGATGTTAT GGGTTCCTGA AGAGCTTAGA GACCGCTGAG 1381 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG 1441 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA 1501 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG 1561 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 1621 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 1681 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 1741 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 1801 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 1861 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 1921 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 1981 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 2041 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 2101 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT 2161 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 2221 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 2281 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 2341 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 2401 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 2461 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 2521 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 2581 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 2641 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 2701 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 2761 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 2821 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 2881 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 2941 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 3001 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 3061 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 3121 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 3181 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 3241 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC 3301 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA 3361 GGTGGAGTGG GAG //