LOCUS       Exported     3154 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3154
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1192..1239
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3087..3103
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3104..3135
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3153
                     /label="ispG"
                     /note="Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate."
                     /translation="MQVSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVACPDERAANAIADIKKRISIPLVVDIHFDYKLALKAIEGGADKIRINPGNIGRREKVEAVVKAAKDKGIPIRIGVNAGSLEKRILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTMRISLSADPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISKIKAPIKVAVLGCAVNGPGEAREADIGIAGARGEGLLFRKGKIVRKVPEETMVEELKKEIDILAEEHYAKLEAEKAKLKEETQKA"
     primer_bind     complement(1244..1260)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1327..1915)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2106..2966)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2967..3071)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG CAAGTTAGCG AGATTACTCA CCGCACTAAA ACCCGTCCGG TCAAAGTGGG
       61 TCCGCTGACC ATTGGCGGCA ATAATGAGGT GGTTATTCAA TCCATGACCA CGACCAAGAC
      121 CCATGATGTG GAGGCCACGG TTGCCGAGAT CAACAGACTG GCGGAGGCCG GTTGCCAGAT
      181 CGTGCGTGTT GCATGTCCTG ACGAGCGTGC GGCGAACGCT ATCGCGGACA TCAAGAAGCG
      241 TATTAGCATC CCGCTGGTGG TCGACATCCA CTTCGATTAT AAGCTGGCGC TGAAGGCGAT
      301 TGAGGGCGGT GCCGACAAGA TCCGTATTAA TCCGGGCAAT ATCGGTCGCC GTGAGAAGGT
      361 TGAAGCGGTG GTGAAAGCCG CAAAGGACAA GGGTATTCCG ATTCGCATTG GTGTGAACGC
      421 AGGCTCCCTG GAAAAGCGTA TTCTGGAGAA GTACGGTTAT CCGACCGCGG ACGGTATGGT
      481 CGAGTCGGCA TTGCATCATA TTAAAATTCT GGAGGACCTG GACTTTCATG ACATTATCGT
      541 GAGCATGAAG GCATCTGATG TGAACCTGGC AATTGAGGCG TATGAAAAGG CGGCGAAAGC
      601 GTTCGATTAT CCGCTGCACC TCGGTATTAC CGAGAGCGGT ACTCTGTTCG CCGGCACCGT
      661 CAAGTCCGCC GCGGGGCTCG GTGCCATTCT GAGCAAAGGT ATTGGCAATA CCATGCGTAT
      721 TAGCCTGAGC GCGGACCCGG TGGAGGAGGT CAAGGTCGCG CGCGAACTGC TCAAGAGCTT
      781 CGGCCTGGCA TCCAATGCGG CGACGCTGAT TAGCTGTCCG ACATGCGGTC GTATTGAGAT
      841 TGACTTGATC AGTATCGCAA ATGAAGTTGA GGAATATATC AGCAAAATCA AGGCGCCGAT
      901 CAAAGTGGCA GTACTTGGCT GCGCGGTTAA CGGTCCGGGC GAGGCGCGCG AGGCGGATAT
      961 CGGGATCGCG GGCGCGAGAG GTGAAGGTCT GCTGTTCCGT AAGGGTAAAA TCGTTCGTAA
     1021 GGTGCCGGAA GAAACGATGG TTGAAGAACT GAAAAAAGAG ATCGACATCC TGGCCGAGGA
     1081 ACACTATGCG AAACTGGAGG CAGAGAAGGC AAAATTAAAA GAGGAAACCC AAAAGGCCTG
     1141 AAGAGCTTAG AGACCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     1201 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     1261 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     1321 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1381 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1441 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1501 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1561 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1621 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1681 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1741 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1801 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1861 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG
     1921 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     1981 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     2041 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     2101 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     2161 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     2221 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     2281 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     2341 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     2401 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     2461 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     2521 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     2581 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     2641 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     2701 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     2761 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     2821 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     2881 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     2941 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     3001 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     3061 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC
     3121 CGGAGGCTTT TGACTTGGTC AGGTGGAGTG GGAG
//