LOCUS       Exported     3835 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3835
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3821
                     /label="dnaG"
                     /note="RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication."
                     /translation="MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFHCFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKRGFSEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETPLFHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKILRRNVEEIILCYDSDKAGYEATLKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFKNDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEISTLSGSLEQEVYVKQLASEFSLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHMLRDRSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDILMLQVNQELSEAELSDYVKKVLNQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLNRSLK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGGCAACCG CATCCCGGAC GAAATCGTAG
     2041 ATCAGGTTCA AAAAAGCGCC GACATCGTTG AGGTGATTGG CGACTACGTC CAACTGAAGA
     2101 AACAGGGCCG TAATTATTTT GGTCTGTGTC CGTTTCACGG CGAGTCAACC CCGAGCTTCA
     2161 GCGTGAGCCC AGATAAACAG ATTTTCCACT GCTTTGGTTG CGGCGCGGGT GGTAACGTTT
     2221 TCAGCTTCCT GCGTCAGATG GAGGGTTACA GCTTTGCGGA GAGCGTGTCC CATCTGGCGG
     2281 ATAAGTACCA AATCGACTTT CCGGACGACA TTACCGTGCA CTCCGGTGCG CGCCCGGAAA
     2341 GCTCCGGCGA ACAAAAAATG GCAGAGGCGC ACGAATTGCT GAAAAAGTTC TACCATCATC
     2401 TGCTGATCAA CACCAAAGAA GGTCAGGAGG CCCTGGACTA CTTGCTGAGC CGCGGTTTTA
     2461 CTAAGGAGCT TATTAACGAA TTCCAGATTG GCTATGCTCT GGACAGCTGG GATTTTATTA
     2521 CGAAATTTTT GGTGAAACGT GGCTTTAGCG AAGCTCAAAT GGAGAAAGCT GGCTTACTTA
     2581 TTCGTCGTGA GGACGGTAGC GGTTATTTTG ATAGATTTCG TAATCGCGTT ATGTTTCCGA
     2641 TTCACGACCA TCACGGTGCC GTGGTGGCGT TCAGCGGTCG TGCGCTCGGT AGCCAGCAGC
     2701 CGAAATATAT GAACAGCCCG GAAACGCCGT TGTTCCACAA AAGCAAACTG TTGTATAATT
     2761 TTTATAAGGC ACGTCTGCAC ATCCGCAAGC AGGAACGCGC AGTGCTGTTT GAGGGTTTTG
     2821 CTGATGTTAT CAGCGCAGTG AGCAGCGACG TGAAGGAGAG TATCGCCACC ATGGGTACTA
     2881 GCTTAACGGA TGACCACGTG AAAATTTTGC GCCGTAATGT CGAAGAGATC ATTCTGTGCT
     2941 ATGACTCTGA TAAAGCGGGT TATGAGGCAA CTCTGAAAGC TAGCGAACTG CTGCAGAAAA
     3001 AAGGCTGCAA AGTCCGCGTG GCCATGATTC CGGATGGTCT GGACCCGGAC GATTACATTA
     3061 AGAAATTCGG TGGCGAGAAG TTCAAAAATG ACATCATTGA TGCTAGCGTT ACGGTGATGG
     3121 CGTTCAAGAT GCAGTACTTT CGTAAAGGCA AAAACCTGAG CGACGAAGGT GACCGTCTGG
     3181 CGTACATTAA GGATGTCCTG AAGGAGATCT CTACCCTCAG CGGCAGCCTG GAACAGGAGG
     3241 TGTATGTTAA ACAACTGGCG TCTGAGTTTA GCTTGAGCCA GGAAAGCCTG ACCGAGCAGC
     3301 TGAGCGTCTT TTCGAAGCAA AATAAACCGG CCGACAACAG CGGTGAAACC AAAACCCGTC
     3361 GTGCCCACCT GACCACCAAG GCGCGCCAAA AACGCTTGCG TCCTGCCTAT GAAAACGCCG
     3421 AGCGCTTGTT ACTGGCGCAC ATGCTTCGCG ATCGTAGCGT GATCAAAAAG GTGATTGATC
     3481 GTGTTGGCTT CCAGTTTAAT ATCGATGAAC ACCGTGCACT GGCGGCGTAT CTGTACGCGT
     3541 TTTATGAAGA GGGCGCAGAG CTGACCCCAC AGCATCTGAT GGCACGCGTT ACGGATGACC
     3601 ACATTTCCCA ATTGTTAAGC GACATTTTAA TGTTGCAAGT CAACCAAGAG TTGAGCGAGG
     3661 CCGAGCTGAG CGACTATGTC AAGAAGGTGC TGAACCAGCG TAACTGGTCC ATGATCAAAG
     3721 AGAAGGAGGC AGAACGCGCA GAAGCTGAGC GCCAGAAGGA TTTCCTGCGT GCGGCGAGCC
     3781 TGGCGCAAGA GATTGTCACT CTGAATCGTA GCTTGAAGTG AAGAGCTTAG AGACC
//