LOCUS       Exported     4063 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4063
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4049
                     /translation="MSKQDLLLEIGLEEMPARFLNESMVQLGDKLTGWLKEKNITHGEVKLFNTPRRLAVFVKDVAEKQDDIKEEAKGPAKKIALDADGNWTKAAIGFSKGQGANVEDLYIKEVKGIEYVFVQKFQAGQETKSLLPELSGLITSLHFPKNMRWGNEDLRYIRPIKWIVALFGQDVIPFSITNVESGRTTQGHRFLGHEVSIESPSAYEEQLKGQHVIADPSVRKQMIQSQLETMAAENNWSIPVDEDLLDEVNHLVEYPTALYGSFESEFLSIPEEVLVTTMKEHQRYFPVKDKNGDLLPHFITVRNGNSHAIENVARGNEKVLRARLSDASFFYKEDQKLNIDANVKKLENIVFHEELGSLADKVRRVTSIAEKLAVRLQADEDTLKHVKRAAEISKFDLVTHMIYEFPELQGIMGEKYARMLGEDEAVAAAVNEHYMPRSAGGETPSTFTGAVVAMADKLDTIASFFSIGVIPTGSQDPYGLRRQASGIVAILLDRNWGISFEELLTFVQTDKENELLDFFTQRLKYVLNAEQIRHDVIDAVLESSELEPYSALHKAQVLEQKLGAPGFKETAEALGRVISISKKGVRGDIQPDLFENEYEAKLFDAYQTAKQNLQENFSKKDYEAALASLAALKEPIDAYFDHTMVIADNESLKANRLAQMVSLADEIKSFANMNALIVK"
                     /label="glyS"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAGCAAACA AGATCTGTTG CTGGAAATCG
     2041 GTCTGGAGGA AATGCCGGCG CGTTTTCTGA ATGAGAGCAT GGTGCAGCTG GGCGACAAGC
     2101 TGACCGGCTG GCTGAAAGAG AAAAACATTA CGCATGGCGA AGTTAAACTG TTTAATACCC
     2161 CTCGTCGTCT GGCTGTTTTC GTGAAGGACG TTGCGGAGAA ACAAGACGAC ATTAAGGAGG
     2221 AGGCCAAAGG CCCTGCCAAA AAGATCGCCT TGGACGCGGA TGGTAATTGG ACTAAAGCTG
     2281 CGATTGGCTT CAGCAAGGGT CAGGGTGCCA ACGTTGAGGA TCTGTACATC AAGGAAGTGA
     2341 AAGGTATTGA ATATGTGTTT GTCCAAAAAT TCCAAGCCGG TCAGGAAACC AAGTCCCTGC
     2401 TGCCGGAACT GTCTGGCCTG ATCACCTCGC TGCACTTCCC GAAGAATATG AGATGGGGTA
     2461 ACGAGGATCT GCGTTACATT CGTCCGATTA AGTGGATCGT CGCGCTGTTC GGCCAGGATG
     2521 TCATCCCGTT CTCCATTACT AATGTGGAAA GCGGTCGTAC TACCCAAGGT CATCGTTTTC
     2581 TTGGCCACGA GGTGAGCATC GAGAGCCCGA GCGCATATGA GGAGCAGCTG AAGGGTCAAC
     2641 ACGTTATCGC CGATCCGAGC GTTCGTAAAC AAATGATCCA GAGCCAACTG GAAACCATGG
     2701 CGGCGGAGAA CAACTGGTCC ATCCCTGTGG ATGAGGATCT GCTGGATGAG GTAAACCATC
     2761 TGGTGGAATA CCCGACCGCG CTGTATGGTA GCTTCGAATC CGAATTTCTG TCGATCCCGG
     2821 AGGAAGTCCT GGTCACGACC ATGAAAGAGC ACCAACGCTA CTTTCCTGTT AAGGATAAAA
     2881 ACGGTGATCT GTTGCCACAC TTTATTACTG TTCGCAACGG CAACAGCCAT GCAATTGAGA
     2941 ACGTTGCACG TGGCAATGAG AAGGTGCTGC GTGCTCGTCT GAGCGACGCG TCCTTCTTTT
     3001 ACAAAGAGGA CCAAAAGTTG AACATCGATG CCAACGTCAA GAAGCTGGAG AATATCGTCT
     3061 TTCATGAGGA GCTGGGTAGC CTGGCAGACA AGGTGCGTCG TGTCACGAGC ATTGCAGAGA
     3121 AGCTGGCGGT CCGTCTGCAA GCAGATGAAG ATACCCTGAA GCATGTCAAA CGCGCAGCGG
     3181 AGATCAGCAA GTTTGATCTG GTCACCCACA TGATTTACGA ATTCCCGGAA CTGCAGGGTA
     3241 TCATGGGTGA GAAGTACGCG CGTATGTTGG GCGAAGATGA GGCCGTTGCG GCCGCTGTTA
     3301 ACGAGCACTA CATGCCGCGT AGCGCCGGTG GTGAGACTCC GAGCACGTTT ACCGGTGCCG
     3361 TTGTTGCAAT GGCGGACAAA CTGGACACTA TCGCTTCCTT CTTTAGCATC GGTGTTATCC
     3421 CAACTGGCTC TCAGGATCCG TACGGTCTGC GTCGTCAGGC CAGCGGCATT GTTGCGATCT
     3481 TGCTGGATCG CAACTGGGGC ATTAGCTTTG AGGAGCTGCT GACGTTCGTG CAGACGGACA
     3541 AGGAGAACGA ACTGCTGGAT TTCTTCACCC AGCGTCTGAA GTATGTCCTC AATGCCGAGC
     3601 AGATTCGTCA CGACGTGATC GACGCTGTGC TGGAGAGCAG CGAACTGGAA CCGTACAGCG
     3661 CGCTGCACAA AGCCCAGGTG CTGGAGCAGA AGCTGGGTGC ACCGGGCTTT AAAGAAACCG
     3721 CGGAGGCCCT GGGTCGCGTC ATTAGCATTA GCAAGAAAGG TGTGCGCGGT GACATTCAAC
     3781 CTGATCTGTT CGAGAATGAA TACGAGGCAA AACTCTTTGA TGCATATCAG ACCGCGAAGC
     3841 AAAATCTGCA AGAAAATTTT AGCAAAAAGG ACTACGAGGC AGCGCTGGCG AGCCTGGCAG
     3901 CGCTGAAGGA GCCGATTGAC GCATATTTCG ACCACACGAT GGTAATCGCT GACAACGAGT
     3961 CGTTAAAAGC GAATCGCCTG GCGCAAATGG TTTCCCTGGC AGACGAGATT AAGTCATTCG
     4021 CAAACATGAA TGCACTGATT GTGAAGTGAA GAGCTTAGAG ACC
//