LOCUS       Exported     4660 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4660
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..4646
                     /label="alaS"
                     /note="Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain."
                     /translation="MKHLTSAEVRQMFLDFFKEKGHAVEPSASLVPHEDPSLLWINSGVATLKKYFDGRVVPENPRIVNAQKAIRTNDIENVGKTARHHTFFEMLGNFSIGDYFKEEAITWAWEFLTSDKWIGFDKELLSVTVHPEDEEAYEFWAKKIGIPEERIIRLEGNFWDIGEGPSGPNTEIFYDRGEAYGNDPEDPELYPGGENDRYLEVWNLVFSEFNHNPDGTYTPLPKKNIDTGMGLERMVSVIQNVPTNFDTDLFVPIIKATESISGETYGKDNVKDTAFKVIADHIRTVAFAVSDGALPSNEGRGYVLRRLLRRAVRYAKTINIHRPFMFDLVPVVAEIMADFYPEVKEKADFIAKVIKTEEERFHETLNEGLAILSEMIKKEKDKGSSVISGADVFKLYDTYGFPVELTEEYAEDENMTVDHEGFEEEMNQQRERARNARQDVGSMQVQGGALRDVTVESTFVGYSQTKADANIIVLLQDGQLIEEAHEGESVQIILDETPFYAESGGQIGDKGYLRSEQAVVRIKDVQKAPNGQHVHEGVVESGTVQKGLHVTAEVEDHMRSGVIKNHTATHLLHQALKDVLGTHVNQAGSLVTENRLRFDFSHFGQVTKEELEQIERIVNEKIWASIPVSIDLKPIAEAKEMGAMALFGEKYGDIVRVVQVGDYSLELCGGCHVRNTAEIGLFKIVSESGIGAGTRRIEAVTGQGAYVEMNSQISVLKQTADELKTNIKEVPKRVAALQAELKDAQRENESLLAKLGNVEAGAILSKVKEVDGVNVLAAKVNAKDMNHLRTMVDELKAKLGSAVIVLGAVQNDKVNISAGVTKDLIEKGLHAGKLVKQAAEVCGGGGGGRPDMAQAGGKQPEKLEEALASVEDWVKSVL"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAACACCT GACCTCCGCG GAGGTCCGTC
     2041 AGATGTTCCT GGACTTTTTC AAGGAGAAAG GTCACGCGGT TGAGCCGAGC GCGAGCTTGG
     2101 TCCCGCACGA GGACCCGAGC CTGCTGTGGA TTAACAGCGG TGTGGCCACC CTGAAAAAAT
     2161 ATTTCGATGG TCGCGTCGTG CCGGAGAATC CACGTATCGT TAATGCCCAA AAGGCGATCC
     2221 GTACCAATGA CATCGAGAAT GTTGGTAAAA CGGCACGCCA CCATACCTTC TTTGAGATGT
     2281 TGGGTAACTT CAGCATTGGT GACTATTTTA AGGAGGAAGC GATTACCTGG GCGTGGGAAT
     2341 TTCTGACGAG CGATAAGTGG ATTGGCTTTG ACAAGGAACT GCTGAGCGTC ACGGTTCACC
     2401 CGGAGGATGA GGAGGCCTAT GAATTCTGGG CTAAGAAAAT CGGTATCCCG GAGGAGCGTA
     2461 TTATTCGTTT GGAGGGCAAT TTTTGGGACA TCGGCGAAGG TCCGAGCGGT CCGAACACGG
     2521 AAATTTTTTA CGATAGAGGC GAGGCGTATG GCAATGATCC AGAGGATCCG GAACTGTACC
     2581 CTGGTGGCGA GAATGATCGC TACTTGGAGG TTTGGAATCT GGTTTTTAGC GAGTTCAACC
     2641 ACAACCCGGA TGGCACGTAC ACGCCACTGC CGAAGAAAAA TATTGACACC GGTATGGGCC
     2701 TGGAGCGTAT GGTCAGCGTG ATTCAGAACG TGCCTACTAA CTTCGATACG GATTTGTTTG
     2761 TTCCGATCAT TAAGGCAACC GAGAGCATTA GCGGCGAAAC CTATGGTAAA GATAACGTCA
     2821 AGGATACCGC GTTTAAAGTG ATTGCAGACC ATATTCGTAC GGTTGCATTT GCGGTGTCTG
     2881 ATGGCGCTCT GCCGAGCAAT GAGGGTCGTG GTTACGTGCT GCGTCGCTTG CTGCGCCGCG
     2941 CGGTTCGTTA TGCGAAAACC ATCAACATTC ATCGTCCGTT CATGTTCGAT CTGGTTCCGG
     3001 TGGTGGCCGA GATCATGGCC GATTTCTACC CGGAAGTGAA AGAGAAGGCT GACTTTATCG
     3061 CGAAAGTTAT CAAAACGGAG GAGGAGCGCT TCCACGAAAC GTTAAATGAG GGTTTGGCGA
     3121 TCTTGAGCGA GATGATTAAA AAAGAGAAGG ACAAGGGTAG CAGCGTGATC AGCGGCGCCG
     3181 ACGTTTTCAA ATTGTATGAT ACCTATGGTT TTCCGGTGGA ACTGACCGAG GAGTACGCCG
     3241 AGGACGAGAA CATGACTGTT GATCACGAGG GTTTCGAAGA AGAGATGAAT CAACAACGCG
     3301 AACGTGCCCG TAACGCTCGT CAGGACGTTG GTTCCATGCA AGTTCAGGGC GGCGCGCTGC
     3361 GTGACGTTAC CGTCGAAAGC ACGTTTGTTG GTTACTCCCA GACGAAAGCG GACGCGAATA
     3421 TTATCGTTCT GTTACAGGAC GGTCAGTTGA TCGAGGAAGC ACACGAAGGT GAGAGCGTTC
     3481 AGATCATCCT GGATGAAACT CCGTTCTACG CGGAGAGCGG CGGTCAGATT GGCGATAAAG
     3541 GCTATCTGCG TAGCGAACAA GCCGTGGTGC GTATCAAAGA CGTGCAGAAA GCTCCTAACG
     3601 GTCAGCACGT CCACGAGGGT GTGGTTGAGA GCGGTACCGT GCAAAAGGGT CTGCATGTGA
     3661 CCGCGGAGGT CGAGGATCAC ATGCGCTCTG GCGTCATTAA GAATCACACC GCAACCCATC
     3721 TGCTGCATCA AGCACTGAAG GACGTTTTGG GCACCCACGT GAACCAGGCG GGCAGCCTGG
     3781 TGACCGAAAA TCGCCTGCGT TTTGACTTTT CACACTTCGG CCAGGTTACC AAAGAGGAAC
     3841 TGGAACAGAT CGAGAGAATT GTCAATGAGA AGATCTGGGC TAGCATCCCT GTCTCCATTG
     3901 ATCTGAAACC GATTGCAGAG GCGAAAGAGA TGGGTGCAAT GGCGCTGTTC GGTGAAAAGT
     3961 ATGGTGACAT CGTTCGCGTT GTGCAAGTGG GCGACTATTC TTTAGAACTG TGTGGTGGCT
     4021 GCCACGTGCG TAATACCGCA GAAATCGGTC TGTTCAAGAT CGTTTCTGAA TCCGGTATCG
     4081 GCGCAGGCAC CCGCCGTATT GAGGCGGTGA CCGGTCAAGG CGCGTATGTC GAAATGAACT
     4141 CGCAGATTTC AGTTTTAAAG CAGACGGCGG ACGAACTGAA AACTAATATT AAGGAAGTGC
     4201 CGAAGCGTGT GGCGGCTCTG CAGGCCGAAT TAAAGGACGC TCAACGCGAA AATGAGTCCC
     4261 TGCTTGCGAA GCTTGGCAAC GTTGAGGCAG GCGCGATCCT TAGCAAGGTT AAAGAAGTGG
     4321 ACGGTGTTAA TGTGTTGGCA GCGAAAGTTA ATGCGAAGGA CATGAACCAT TTGCGTACGA
     4381 TGGTTGACGA ACTGAAAGCG AAACTGGGTT CCGCGGTGAT CGTACTGGGC GCGGTCCAAA
     4441 ATGATAAGGT CAATATTTCT GCGGGCGTTA CCAAGGACTT GATCGAAAAG GGTTTACACG
     4501 CCGGCAAACT GGTCAAACAG GCGGCCGAAG TTTGCGGCGG CGGTGGCGGT GGCCGTCCAG
     4561 ACATGGCGCA GGCGGGTGGC AAACAGCCGG AGAAACTGGA GGAAGCACTG GCGTCGGTTG
     4621 AAGATTGGGT GAAAAGCGTT CTGTGAAGAG CTTAGAGACC
//