LOCUS       Exported     3136 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3136
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1174..1221
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3069..3085
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3086..3117
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3135
                     /label="mnmA"
                     /note="Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34."
                     /translation="MEKRPEDTRVVVGMSGGVDSSVAALLLKEQGYDVIGIFMKNWDDTDENGFCTATEDYEDVIRVCNQIGIPYYAVNFEKQYYEKVFQYFLDEYKAGRTPNPDVLCNKEIKFKAFLEHALSLGADYLATGHYARVDRSGGKVRMLRGIDENKDQTYFLNQLTEDTLSKVMFPIGELQKSRVREIAKEAELATATKKDSTGICFIGERNFKTFLSQYLPAQPGDMMTMDGEVKGRHDGLMYYTIGQRHGLGIGGSGEPWFAVGKDLEKNILYVDQGFHNPLLYSDKITATNISWVRSDIMKGEEISCTAKFRYRQEDHKVTVRMTGEGEAEVIFDEQVRAVTPGQAVVFYDGEECLGGGTIDDVYKDGTKLWYV"
     primer_bind     complement(1226..1242)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1309..1897)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2088..2948)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2949..3053)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG GAAAAACGTC CGGAGGACAC CCGTGTTGTT GTCGGTATGA GCGGTGGCGT
       61 GGATAGCTCT GTGGCCGCGC TGCTGCTGAA AGAGCAAGGC TATGATGTGA TCGGTATTTT
      121 CATGAAAAAC TGGGACGACA CCGACGAAAA TGGTTTCTGT ACCGCGACCG AGGACTATGA
      181 GGACGTGATC CGTGTGTGCA ACCAGATTGG TATTCCATAT TATGCGGTCA ATTTTGAGAA
      241 ACAGTACTAC GAAAAAGTTT TCCAATACTT CCTGGATGAA TATAAGGCCG GCCGTACCCC
      301 GAACCCGGAT GTGCTGTGCA ATAAAGAAAT CAAGTTTAAA GCTTTCCTGG AGCATGCGCT
      361 GTCTCTGGGC GCCGACTATC TGGCAACCGG CCATTACGCA CGTGTGGACC GCTCCGGTGG
      421 CAAGGTACGT ATGCTGCGTG GCATTGATGA AAACAAGGAT CAGACCTATT TCCTGAACCA
      481 ACTCACTGAG GACACCCTCA GCAAAGTTAT GTTTCCGATC GGCGAGTTGC AAAAAAGCCG
      541 TGTCCGTGAG ATCGCGAAGG AAGCTGAACT GGCGACCGCA ACGAAGAAGG ACAGCACGGG
      601 TATCTGCTTC ATTGGCGAAC GTAATTTCAA GACCTTCCTG TCTCAGTATC TGCCTGCCCA
      661 GCCGGGCGAC ATGATGACGA TGGACGGTGA GGTTAAGGGC CGCCACGATG GCCTGATGTA
      721 CTACACGATT GGTCAACGTC ACGGCCTGGG TATTGGTGGC AGCGGTGAAC CGTGGTTTGC
      781 GGTCGGCAAG GATCTGGAGA AGAACATCCT GTACGTCGAC CAGGGCTTCC ATAACCCTCT
      841 GCTGTACAGC GATAAGATTA CCGCCACGAA TATCAGCTGG GTTCGTTCTG ATATCATGAA
      901 AGGTGAGGAA ATCAGCTGTA CCGCGAAATT TCGTTACCGT CAAGAAGATC ACAAAGTAAC
      961 CGTGCGTATG ACCGGCGAGG GTGAGGCGGA AGTTATCTTT GATGAACAGG TGCGTGCGGT
     1021 GACCCCGGGC CAGGCGGTGG TCTTTTACGA CGGCGAAGAG TGCCTGGGTG GTGGCACGAT
     1081 CGACGACGTG TATAAGGATG GCACCAAATT GTGGTACGTG TGAAGAGCTT AGAGACCGCT
     1141 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1201 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1261 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1321 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1381 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1441 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1501 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1561 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1621 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1681 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1741 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1801 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1861 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1921 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1981 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2041 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2101 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2161 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2221 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2281 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2341 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2401 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2461 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2521 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2581 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2641 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2701 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2761 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2821 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2881 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2941 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3001 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3061 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3121 TCAGGTGGAG TGGGAG
//