LOCUS       Exported     3802 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3802
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3788
                     /translation="MFGRTYYCGDITEKAIGESVTLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKEALAIAEGIRNEYVLDIQGKVVAREEGTVNPNLKTGAIEIHADGVNVLNAAKTPPFAISDQAEEVSEDVRLKHRYLDLRRPAMFQTMQLRHNVTKAVRSFLDENGFLDIETPILTGSTPEGARDYLVPSRVHEGEFYALPQSPQLFKQLLMVSGIERYYQIARCFRDEDLRADRQPEFTQIDIEMSFMSQEDIMSLAEEMMAKVMRETKGEELQLPLPRMTYDEAMNKYGSDKPDTRFDMLLTDVSDIVKDTEFKVFSSAVANGGVVKAINVKGGAGDYSRKDIDALGAFAANYGAKGLAWVKVEADGVKGPIAKFFDEEKQSKLIEALDAAEGDLLLFGADQFEVVAASLGALRLKLGKERGLIDEKLFNFLWVIDWPLLEHDPEEGRFYAAHHPFTMPVREDLELIETAPEDMKAQAYDLVLNGYELGGGSIRIFEKDIQEKMFALLGFSPEEAAEQFGFLLEAFEYGAPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKTASASCLMTEAPGEVSDAQLDELHLSIKKKVKN"
                     /label="aspS"
                     /note="Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn)."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTTTGGTCG CACCTACTAT TGCGGTGATA
     2041 TTACCGAGAA GGCGATTGGT GAAAGCGTGA CGCTGAAAGG TTGGGTGCAA AAGCGTCGCG
     2101 ACCTGGGTGG CCTGATCTTT ATTGATCTGC GCGACCGTAC CGGCATCGTG CAAGTCGTTT
     2161 TCAACCCGGA TGTCTCGAAA GAAGCACTGG CTATCGCGGA GGGTATCCGC AACGAATACG
     2221 TTCTGGATAT TCAAGGCAAG GTTGTGGCTC GCGAAGAGGG CACTGTTAAT CCTAACCTGA
     2281 AAACCGGTGC GATCGAGATC CATGCGGACG GTGTAAACGT GTTGAATGCG GCTAAGACGC
     2341 CGCCGTTTGC GATCAGCGAC CAAGCAGAGG AGGTCAGCGA GGATGTCCGT TTGAAACACC
     2401 GTTACTTGGA TCTGCGCCGT CCGGCAATGT TTCAAACGAT GCAACTGCGC CACAACGTTA
     2461 CCAAGGCGGT GCGTAGCTTT CTCGATGAAA ACGGTTTCCT GGATATCGAA ACGCCGATCT
     2521 TAACCGGCAG CACCCCGGAA GGTGCGCGCG ACTATCTGGT GCCTTCCCGC GTTCACGAGG
     2581 GTGAGTTCTA TGCGTTGCCG CAGAGCCCGC AGCTGTTCAA ACAACTGCTG ATGGTCAGCG
     2641 GTATCGAGCG TTACTACCAG ATTGCGCGTT GTTTTCGTGA CGAGGATCTG CGTGCGGACC
     2701 GCCAACCGGA ATTCACGCAA ATCGACATCG AGATGAGCTT TATGAGCCAG GAGGATATCA
     2761 TGTCCCTGGC GGAGGAGATG ATGGCGAAAG TGATGCGCGA AACCAAGGGC GAAGAATTAC
     2821 AACTGCCGCT GCCGCGTATG ACCTACGACG AAGCAATGAA CAAGTACGGT TCCGATAAGC
     2881 CGGACACGCG CTTTGACATG CTGCTGACGG ACGTTTCCGA CATCGTGAAG GATACCGAGT
     2941 TTAAAGTGTT CAGCAGCGCG GTGGCGAACG GCGGCGTTGT GAAGGCCATC AACGTGAAGG
     3001 GTGGCGCTGG CGACTACAGC CGTAAGGACA TCGATGCGCT GGGCGCGTTC GCGGCAAATT
     3061 ACGGCGCGAA AGGTCTGGCG TGGGTGAAGG TTGAAGCCGA CGGTGTTAAG GGTCCGATTG
     3121 CGAAGTTCTT CGATGAGGAA AAACAGAGCA AGCTGATTGA AGCCCTGGAC GCGGCCGAAG
     3181 GCGATCTGCT GCTGTTCGGC GCGGATCAGT TTGAGGTTGT TGCGGCCAGC CTGGGTGCAC
     3241 TGCGCTTGAA ACTGGGCAAA GAGCGTGGCC TGATCGACGA GAAACTGTTT AACTTTTTGT
     3301 GGGTCATTGA TTGGCCGTTG CTGGAGCACG ATCCTGAGGA GGGTCGCTTC TACGCAGCTC
     3361 ATCACCCGTT CACCATGCCG GTACGTGAGG ATCTGGAGCT CATTGAAACG GCGCCGGAGG
     3421 ACATGAAAGC CCAGGCATAT GATCTGGTTC TGAATGGTTA TGAGCTGGGT GGTGGCAGCA
     3481 TCCGTATTTT CGAAAAGGAC ATTCAGGAAA AGATGTTTGC GCTGCTGGGC TTCTCGCCGG
     3541 AGGAAGCAGC AGAACAATTC GGTTTCCTGC TGGAAGCATT CGAGTATGGC GCTCCGCCGC
     3601 ACGGTGGTAT CGCGCTGGGC CTGGATCGCC TGGTTATGCT TCTGGCGGGT CGTACCAATC
     3661 TGCGCGACAC TATCGCGTTT CCGAAAACGG CAAGCGCGAG CTGTCTGATG ACGGAAGCAC
     3721 CAGGCGAAGT CTCGGATGCG CAGCTGGACG AGCTGCACCT GTCAATTAAA AAGAAAGTTA
     3781 AAAACTGAAG AGCTTAGAGA CC
//