LOCUS Exported 3802 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3802 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 2010..3788 /translation="MFGRTYYCGDITEKAIGESVTLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKEALAIAEGIRNEYVLDIQGKVVAREEGTVNPNLKTGAIEIHADGVNVLNAAKTPPFAISDQAEEVSEDVRLKHRYLDLRRPAMFQTMQLRHNVTKAVRSFLDENGFLDIETPILTGSTPEGARDYLVPSRVHEGEFYALPQSPQLFKQLLMVSGIERYYQIARCFRDEDLRADRQPEFTQIDIEMSFMSQEDIMSLAEEMMAKVMRETKGEELQLPLPRMTYDEAMNKYGSDKPDTRFDMLLTDVSDIVKDTEFKVFSSAVANGGVVKAINVKGGAGDYSRKDIDALGAFAANYGAKGLAWVKVEADGVKGPIAKFFDEEKQSKLIEALDAAEGDLLLFGADQFEVVAASLGALRLKLGKERGLIDEKLFNFLWVIDWPLLEHDPEEGRFYAAHHPFTMPVREDLELIETAPEDMKAQAYDLVLNGYELGGGSIRIFEKDIQEKMFALLGFSPEEAAEQFGFLLEAFEYGAPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKTASASCLMTEAPGEVSDAQLDELHLSIKKKVKN" /label="aspS" /note="Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn)." primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTTTGGTCG CACCTACTAT TGCGGTGATA 2041 TTACCGAGAA GGCGATTGGT GAAAGCGTGA CGCTGAAAGG TTGGGTGCAA AAGCGTCGCG 2101 ACCTGGGTGG CCTGATCTTT ATTGATCTGC GCGACCGTAC CGGCATCGTG CAAGTCGTTT 2161 TCAACCCGGA TGTCTCGAAA GAAGCACTGG CTATCGCGGA GGGTATCCGC AACGAATACG 2221 TTCTGGATAT TCAAGGCAAG GTTGTGGCTC GCGAAGAGGG CACTGTTAAT CCTAACCTGA 2281 AAACCGGTGC GATCGAGATC CATGCGGACG GTGTAAACGT GTTGAATGCG GCTAAGACGC 2341 CGCCGTTTGC GATCAGCGAC CAAGCAGAGG AGGTCAGCGA GGATGTCCGT TTGAAACACC 2401 GTTACTTGGA TCTGCGCCGT CCGGCAATGT TTCAAACGAT GCAACTGCGC CACAACGTTA 2461 CCAAGGCGGT GCGTAGCTTT CTCGATGAAA ACGGTTTCCT GGATATCGAA ACGCCGATCT 2521 TAACCGGCAG CACCCCGGAA GGTGCGCGCG ACTATCTGGT GCCTTCCCGC GTTCACGAGG 2581 GTGAGTTCTA TGCGTTGCCG CAGAGCCCGC AGCTGTTCAA ACAACTGCTG ATGGTCAGCG 2641 GTATCGAGCG TTACTACCAG ATTGCGCGTT GTTTTCGTGA CGAGGATCTG CGTGCGGACC 2701 GCCAACCGGA ATTCACGCAA ATCGACATCG AGATGAGCTT TATGAGCCAG GAGGATATCA 2761 TGTCCCTGGC GGAGGAGATG ATGGCGAAAG TGATGCGCGA AACCAAGGGC GAAGAATTAC 2821 AACTGCCGCT GCCGCGTATG ACCTACGACG AAGCAATGAA CAAGTACGGT TCCGATAAGC 2881 CGGACACGCG CTTTGACATG CTGCTGACGG ACGTTTCCGA CATCGTGAAG GATACCGAGT 2941 TTAAAGTGTT CAGCAGCGCG GTGGCGAACG GCGGCGTTGT GAAGGCCATC AACGTGAAGG 3001 GTGGCGCTGG CGACTACAGC CGTAAGGACA TCGATGCGCT GGGCGCGTTC GCGGCAAATT 3061 ACGGCGCGAA AGGTCTGGCG TGGGTGAAGG TTGAAGCCGA CGGTGTTAAG GGTCCGATTG 3121 CGAAGTTCTT CGATGAGGAA AAACAGAGCA AGCTGATTGA AGCCCTGGAC GCGGCCGAAG 3181 GCGATCTGCT GCTGTTCGGC GCGGATCAGT TTGAGGTTGT TGCGGCCAGC CTGGGTGCAC 3241 TGCGCTTGAA ACTGGGCAAA GAGCGTGGCC TGATCGACGA GAAACTGTTT AACTTTTTGT 3301 GGGTCATTGA TTGGCCGTTG CTGGAGCACG ATCCTGAGGA GGGTCGCTTC TACGCAGCTC 3361 ATCACCCGTT CACCATGCCG GTACGTGAGG ATCTGGAGCT CATTGAAACG GCGCCGGAGG 3421 ACATGAAAGC CCAGGCATAT GATCTGGTTC TGAATGGTTA TGAGCTGGGT GGTGGCAGCA 3481 TCCGTATTTT CGAAAAGGAC ATTCAGGAAA AGATGTTTGC GCTGCTGGGC TTCTCGCCGG 3541 AGGAAGCAGC AGAACAATTC GGTTTCCTGC TGGAAGCATT CGAGTATGGC GCTCCGCCGC 3601 ACGGTGGTAT CGCGCTGGGC CTGGATCGCC TGGTTATGCT TCTGGCGGGT CGTACCAATC 3661 TGCGCGACAC TATCGCGTTT CCGAAAACGG CAAGCGCGAG CTGTCTGATG ACGGAAGCAC 3721 CAGGCGAAGT CTCGGATGCG CAGCTGGACG AGCTGCACCT GTCAATTAAA AAGAAAGTTA 3781 AAAACTGAAG AGCTTAGAGA CC //