LOCUS       Exported     3388 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3388
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1426..1473
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3321..3337
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3338..3369
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             100..3387
                     /translation="MHILVVGVDYKSAPIEIREKVSFQPNELAEAMVQLKEEKSILENIIVSTCNRTEIYAVVDQLHTGRYYIKKFLADWFQLSKEELSPFLTFYESDAAVEHLFRVACGLDSMVIGETQILGQVRDSFKTAQQEKTIGTIFNELFKQAVTVGKRTHAETDIGSNAVSVSYAAVELAKKIFGNLSSKHILILGAGKMGELAAENLHGQGIGKVTVINRTYLKAKELADRFSGEARSLNQLESALAEADILISSTGASEFVVSKEMMENANKLRKGRPLFMVDIAVPRDLDPALNDLEGVFLYDIDDLEGIVEANMKERRETAEKVELLIEETIVEFKQWMNTLGVVPVISALREKALAIQSETMDSIERKLPHLSTREKKLLNKHTKSIINQMLRDPILKVKELAADADSEEKLALFMQIFDIEEAAGRQMMKTVESSQKVHSFKKAESKAGFSPLVSE"
                     /label="hemA"
                     /note="Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA)."
     primer_bind     complement(1478..1494)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1561..2149)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2340..3200)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3201..3305)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG CATATCCTCG TCGTGGGCGT TGATTACAAG AGCGCGCCGA TCGAAATCCG
       61 TGAGAAAGTC TCCTTCCAAC CGAATGAACT GGCAGAAGCC ATGGTCCAGC TGAAAGAGGA
      121 AAAAAGCATC CTGGAGAACA TTATCGTTAG CACCTGTAAC CGTACCGAAA TCTACGCAGT
      181 GGTCGACCAA CTGCACACTG GTCGTTACTA CATCAAAAAG TTCCTGGCAG ATTGGTTTCA
      241 ACTGTCAAAG GAGGAGCTGA GCCCGTTTCT GACCTTCTAT GAGAGTGATG CTGCCGTTGA
      301 GCACCTGTTC CGTGTCGCGT GCGGCCTGGA CTCTATGGTC ATTGGCGAAA CCCAGATCCT
      361 CGGCCAGGTT CGCGACAGCT TCAAAACTGC ACAGCAAGAA AAGACGATTG GCACTATTTT
      421 CAACGAGTTG TTTAAACAGG CCGTTACGGT CGGTAAACGT ACCCATGCAG AAACGGATAT
      481 CGGTAGCAAC GCGGTGTCTG TAAGCTATGC AGCGGTTGAA CTGGCAAAGA AGATCTTTGG
      541 TAACCTGTCG AGCAAGCACA TTCTGATTTT AGGCGCCGGC AAAATGGGTG AGCTGGCTGC
      601 TGAGAATCTG CACGGTCAAG GTATTGGTAA GGTTACCGTG ATTAATCGTA CGTACCTGAA
      661 AGCCAAAGAA CTGGCTGACC GTTTTAGCGG TGAGGCGCGT AGCCTGAACC AGCTGGAAAG
      721 CGCGTTGGCA GAGGCAGACA TCCTGATCAG CAGCACGGGC GCGAGCGAGT TCGTCGTGAG
      781 CAAGGAAATG ATGGAAAATG CCAACAAGCT GCGTAAGGGT CGCCCGCTGT TCATGGTCGA
      841 CATTGCCGTT CCGCGCGATC TGGATCCGGC ACTGAACGAT CTGGAGGGTG TGTTCCTGTA
      901 TGATATCGAT GACCTGGAGG GCATCGTTGA GGCTAACATG AAGGAGCGTC GTGAAACGGC
      961 CGAGAAGGTG GAACTGTTGA TCGAAGAAAC CATCGTCGAG TTTAAGCAGT GGATGAATAC
     1021 TCTGGGTGTC GTTCCGGTGA TTAGCGCACT GCGTGAGAAG GCGCTGGCTA TCCAGTCTGA
     1081 AACCATGGAT TCCATCGAAC GTAAGCTGCC GCATCTGAGC ACCCGTGAGA AGAAGCTGCT
     1141 GAACAAACAC ACGAAAAGCA TCATTAATCA AATGTTGCGT GATCCTATTC TGAAAGTTAA
     1201 GGAGCTGGCG GCAGACGCGG ATTCCGAAGA GAAGCTGGCG CTGTTTATGC AAATTTTCGA
     1261 TATCGAGGAA GCAGCAGGCC GTCAAATGAT GAAAACGGTG GAGAGCTCGC AAAAGGTCCA
     1321 TAGCTTCAAA AAGGCCGAGA GCAAAGCAGG CTTCAGCCCG CTGGTGAGCG AGTGAAGAGC
     1381 TTAGAGACCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1441 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1501 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     1561 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1621 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1681 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1741 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1801 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1861 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1921 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1981 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     2041 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2101 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     2161 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2221 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     2281 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2341 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2401 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     2461 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     2521 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     2581 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     2641 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     2701 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     2761 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     2821 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2881 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2941 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     3001 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     3061 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3121 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3181 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3241 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3301 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3361 CTTTTGACTT GGTCAGGTGG AGTGGGAG
//