LOCUS       Exported     4438 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4438
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4424
                     /label="pheT"
                     /note=""
                     /translation="MFVSYKWLEDYVDLKGMDPAVLAEKITRAGIEVEGIEYKGEGIKGVVIGHVLEREQHPNADKLNKCLVDIGAEAPVQIICGAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEESNGMICSLQELGIESKLVAKEYAEGIFVFPNDAETGSDALAALQLDDAILELGLTPNRADAMNMLGVAYEVAAILDTEVKLPQTDYPAASEQASDYISVKIEDQEANPLYTAKIIKNVTIAPSPLWMQTKLMNAGIRPHNNVVDITNFVLLEYGQPLHAFDYDRFGSKEVVVRKAAENEMIVTLDDQERKLSADHLVITNGTKAQAVAGVMGGAESEVQEDTKTILLEAAYFNGQKVRKASKDLGLRSESSVRFEKGIDPARVRLAAERAAQLIHLYAGGEVLAGTVEEDHLTIEANNIHVSADKVSSVLGLTISKEELISIYKRLGFTVGEADDLLVVTVPSRRGDITIEEDLIEEAARLYGYDNIPSTLPETAGTTGGLTPYQAKRRKVRRFLEGAGLSQAITYSLTNEKKATAFAIEKSLNTVLALPMSEERSILRHSLVPNLLDSVSYNLARQTDSVALYEVGSVFLTKEEDTKPVETERVAGAVTGLWRKQLWQGEKKPVDFFVVKGIVEGLLDKLNVLDSIEFVQSERKQLHPGRTANILLNGSLIGFIGQVHPSLEKELDIKETYVFELDLHALLAAETAPLVYTAIPKYPSVTRDIALVTDKTVTSGQLESVIKEAGGKLLKEVTVFDVYEGEHMEEGKKSVAFSLQYVNPEQTLTEEEVTKAHSKVLKALEDTYQAVLRG"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTTCGTGTC TTACAAATGG CTTGAGGACT
     2041 ATGTGGATCT GAAGGGTATG GATCCGGCGG TGCTGGCAGA GAAGATCACG CGTGCAGGCA
     2101 TTGAAGTGGA AGGTATTGAG TATAAGGGTG AAGGTATCAA GGGTGTCGTC ATCGGTCACG
     2161 TTCTGGAGAG AGAGCAGCAC CCGAACGCCG ATAAACTGAA CAAATGCCTG GTTGACATTG
     2221 GTGCTGAGGC GCCGGTGCAA ATCATCTGCG GTGCGCCGAA TGTCGATAAG GGTCAGAAAG
     2281 TTGCGGTGGC TACCGTTGGT GCGGTGCTGC CGGGTAACTT TAAAATCAAG AAAGCGAAAC
     2341 TTCGTGGTGA AGAAAGCAAT GGTATGATTT GTAGCCTGCA GGAACTGGGC ATCGAGAGCA
     2401 AACTGGTTGC AAAAGAGTAC GCTGAGGGTA TCTTCGTCTT TCCGAATGAC GCCGAAACCG
     2461 GTAGCGACGC CCTGGCCGCA CTGCAGCTCG ACGACGCGAT TCTGGAGCTG GGTCTGACTC
     2521 CGAATCGTGC GGATGCTATG AACATGCTGG GCGTTGCATA TGAGGTTGCG GCCATCTTGG
     2581 ATACCGAAGT GAAGCTGCCG CAAACGGACT ACCCGGCAGC GAGCGAGCAG GCCAGCGATT
     2641 ACATTAGCGT TAAAATTGAG GACCAAGAGG CAAATCCGCT GTATACGGCT AAAATTATCA
     2701 AAAACGTCAC CATTGCACCA TCTCCGCTTT GGATGCAAAC GAAGCTGATG AATGCCGGTA
     2761 TCCGCCCGCA TAATAACGTG GTCGATATCA CGAACTTCGT GCTGTTGGAG TATGGTCAAC
     2821 CGTTGCACGC GTTTGATTAT GACCGTTTCG GCAGCAAAGA AGTTGTCGTC CGTAAAGCGG
     2881 CGGAAAACGA GATGATTGTG ACGCTCGATG ATCAGGAGCG CAAACTGAGC GCAGATCATC
     2941 TGGTCATCAC CAATGGTACC AAGGCACAAG CAGTTGCGGG CGTTATGGGT GGCGCAGAGA
     3001 GCGAAGTACA GGAGGACACC AAAACGATTC TGCTGGAAGC CGCGTACTTT AACGGCCAGA
     3061 AGGTGCGCAA AGCAAGCAAG GATCTGGGTT TGCGCAGCGA ATCTAGCGTC CGTTTTGAGA
     3121 AGGGTATCGA CCCGGCACGT GTCCGTCTGG CAGCCGAACG TGCGGCCCAG CTGATCCATC
     3181 TGTACGCCGG CGGTGAGGTG TTAGCGGGTA CTGTTGAGGA GGATCACCTG ACGATTGAAG
     3241 CCAATAATAT CCATGTGAGC GCGGACAAGG TGAGCTCTGT ACTGGGTCTG ACGATCAGCA
     3301 AGGAAGAACT GATCAGCATT TACAAACGCC TGGGTTTCAC GGTGGGCGAG GCGGATGACT
     3361 TGCTCGTTGT CACCGTTCCA TCTCGCCGTG GTGATATCAC CATTGAGGAG GATCTGATTG
     3421 AGGAGGCCGC ACGTCTGTAC GGCTACGACA ACATCCCGAG CACGCTGCCG GAAACCGCCG
     3481 GCACCACGGG TGGCCTGACG CCGTACCAAG CGAAACGTCG TAAAGTGCGT CGCTTCCTGG
     3541 AGGGCGCTGG CCTGAGCCAA GCTATCACCT ACTCCTTGAC CAACGAGAAA AAAGCGACCG
     3601 CCTTTGCAAT CGAAAAGAGC CTGAATACGG TTTTAGCGCT GCCGATGAGC GAAGAGAGAT
     3661 CCATTCTGCG TCATTCCCTG GTGCCGAATC TGCTGGACAG TGTCTCTTAT AACCTGGCAC
     3721 GCCAAACCGA TAGCGTGGCA CTGTACGAGG TTGGCTCTGT GTTTCTGACC AAAGAAGAGG
     3781 ACACTAAGCC GGTTGAAACC GAGCGCGTCG CTGGTGCTGT TACGGGTCTG TGGCGTAAGC
     3841 AGCTGTGGCA AGGCGAAAAG AAACCGGTTG ATTTCTTTGT CGTCAAGGGC ATTGTTGAGG
     3901 GCCTGCTGGA CAAGCTGAAC GTCCTGGATT CGATTGAGTT TGTCCAGTCT GAGCGCAAGC
     3961 AGCTGCATCC GGGTCGTACC GCGAACATCC TGTTGAATGG CAGCCTGATT GGTTTTATTG
     4021 GCCAAGTGCA CCCAAGCCTG GAGAAAGAGC TGGACATCAA GGAAACCTAC GTTTTTGAGC
     4081 TGGATCTGCA CGCGCTGCTG GCAGCAGAAA CCGCGCCTCT GGTTTACACC GCGATTCCTA
     4141 AGTACCCGAG CGTGACTCGT GACATTGCCC TGGTCACGGA CAAAACGGTC ACCTCTGGTC
     4201 AACTGGAAAG CGTAATTAAG GAGGCCGGTG GTAAGCTGCT GAAGGAGGTC ACGGTTTTTG
     4261 ACGTCTACGA AGGCGAACAC ATGGAGGAAG GTAAGAAAAG CGTTGCGTTT TCTCTGCAAT
     4321 ATGTTAATCC GGAGCAGACT TTGACTGAGG AGGAGGTCAC TAAAGCTCAT TCGAAGGTGC
     4381 TGAAAGCGCT GGAGGACACC TACCAAGCGG TGCTGCGTGG CTGAAGAGCT TAGAGACC
//