LOCUS Exported 3055 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3055 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1093..1140 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2988..3004 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3005..3036 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3054 /label="pheS" /note="" /translation="MEEKLKQLEQEALEQVEAASSLKVVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIANAIADKNEKLEEEEMKQKLAGQTIDVTLPGNPVAVGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFESLNLPKEHPARDMQDSFYITEETLMRTQTSPVQTRTMEKHEGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDKNISMSDLKGTLELVAKKMFGQDREIRLRPSFFPFTEPSVEVDVTCFKCGGNGCSVCKGTGWIEILGAGMVHPNVLKMAGFDPKEYQGFAFGMGVERIAMLKYGIDDIRHFYTNDVRFISQFKQA" primer_bind complement(1145..1161) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1228..1816) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2007..2867) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2868..2972) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 GTCTCAAATG GAAGAAAAGC TGAAACAACT GGAGCAGGAG GCACTGGAAC AGGTGGAAGC 61 TGCGAGCTCC CTGAAAGTTG TTAACGATAT CCGTGTTCAG TACCTGGGCA AAAAGGGTCC 121 GATTACCGAG GTTCTTAGAG GTATGGGCAA GCTGTCTGCA GAGGAACGTC CGAAGATGGG 181 TGCATTGGCG AACGAAGTCC GTGAACGTAT CGCGAACGCA ATTGCAGATA AGAATGAAAA 241 GTTGGAAGAG GAGGAGATGA AACAGAAATT GGCGGGTCAG ACCATTGATG TTACTCTGCC 301 GGGTAACCCG GTGGCGGTTG GCGGCCGCCA TCCGCTGACC GTTGTCATCG AGGAGATCGA 361 GGATCTGTTC ATCGGTATGG GTTATACGGT GGAAGAGGGT CCAGAGGTTG AAACGGACTA 421 TTACAATTTC GAGTCTCTGA ATCTGCCGAA GGAGCACCCG GCCCGCGATA TGCAAGACAG 481 CTTTTATATC ACGGAGGAAA CCCTGATGCG CACCCAAACT TCGCCGGTGC AAACCCGTAC 541 CATGGAAAAA CATGAAGGTA AAGGTCCGGT TAAAATCATT TGCCCGGGCA AGGTTTATCG 601 TCGTGATAAC GATGATGCCA CGCATAGCCA CCAGTTTATG CAAATCGAAG GTCTGGTGGT 661 TGACAAGAAT ATTAGCATGT CTGACCTGAA AGGTACGCTG GAACTGGTCG CAAAGAAGAT 721 GTTCGGTCAG GATCGCGAGA TCCGTCTGCG CCCGAGCTTT TTCCCATTCA CGGAGCCGAG 781 CGTTGAGGTG GACGTGACGT GCTTTAAGTG CGGCGGTAAC GGTTGCTCGG TGTGTAAGGG 841 TACCGGTTGG ATCGAAATCC TGGGTGCGGG CATGGTGCAC CCGAATGTTC TGAAGATGGC 901 GGGTTTCGAT CCGAAGGAGT ACCAGGGTTT TGCATTCGGT ATGGGCGTTG AACGTATCGC 961 CATGCTGAAG TATGGCATCG ACGACATTCG TCATTTCTAT ACGAATGACG TGCGTTTTAT 1021 CAGCCAGTTC AAGCAGGCCT GAAGAGCTTA GAGACCGCTG AGGTGTCAAT CGTCGGAGCC 1081 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG 1141 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT 1201 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 1261 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 1321 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 1381 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 1441 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 1501 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 1561 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 1621 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 1681 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 1741 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 1801 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG 1861 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 1921 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 1981 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 2041 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 2101 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA 2161 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 2221 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 2281 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 2341 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 2401 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 2461 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 2521 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 2581 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 2641 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 2701 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 2761 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 2821 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 2881 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 2941 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC 3001 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG //