LOCUS       Exported     2893 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2893
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      931..978
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2826..2842
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2843..2874
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..2892
                     /label="accD"
                     /note="Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA."
                     /translation="MLKDIFTKKKKYASVPSDQAKHDVPEGIMTKCPKCKKIMLTKELDKNMRVCMNCDYHFPMNAKQRIESLMDEQSFEEFNQGMLSENPLGFPGYLEKLEKDREKTSLNEAVVTGKGTIGGHPAVVAVMDSSFRMGSMGSVVGEKITLAIEKAKADKVPFIIFTASGGARMQEGVLSLMQMAKTSSALKLFSEEQGLIISVMTHPTTGGVSASFASLGDYNFAEPGALIGFAGRRIIEQTIGEKLPEDFQTAEFLLKHGQLDAVIHRDDMKKTLENLLDMHQTGGDIEWLQD"
     primer_bind     complement(983..999)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1066..1654)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1845..2705)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2706..2810)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG CTGAAAGACA TCTTTACGAA AAAGAAAAAG TATGCGAGCG TGCCGAGCGA
       61 CCAGGCCAAA CATGATGTCC CGGAGGGCAT TATGACCAAG TGCCCGAAGT GTAAGAAAAT
      121 CATGCTGACC AAGGAACTGG ACAAAAACAT GCGTGTTTGT ATGAACTGTG ATTACCACTT
      181 CCCGATGAAC GCGAAACAGC GTATCGAGAG CCTGATGGAT GAGCAGTCCT TCGAGGAGTT
      241 CAACCAGGGC ATGCTGAGCG AGAACCCTCT GGGCTTCCCG GGTTACCTGG AAAAGCTGGA
      301 AAAGGATCGC GAAAAGACGA GCCTGAATGA AGCTGTTGTC ACCGGCAAGG GCACCATCGG
      361 TGGCCATCCG GCAGTTGTGG CCGTCATGGA TAGCAGCTTC CGTATGGGTA GCATGGGTAG
      421 CGTTGTGGGT GAGAAGATTA CCCTGGCGAT TGAAAAAGCG AAGGCCGATA AAGTTCCGTT
      481 CATCATCTTT ACGGCCAGCG GCGGCGCGCG TATGCAAGAG GGTGTTCTGA GCCTGATGCA
      541 GATGGCCAAG ACCAGCAGCG CCCTGAAATT GTTCAGCGAG GAACAAGGCT TAATCATCAG
      601 CGTTATGACC CACCCGACGA CGGGTGGCGT TAGCGCCAGC TTCGCGAGCC TGGGTGACTA
      661 CAATTTCGCC GAACCAGGCG CGCTGATTGG CTTCGCGGGC CGTCGTATTA TTGAGCAAAC
      721 CATCGGTGAA AAGCTGCCGG AAGATTTCCA AACCGCGGAG TTTCTGCTGA AGCACGGCCA
      781 ATTGGACGCA GTGATTCACC GCGACGATAT GAAAAAGACC CTGGAAAACT TGTTGGACAT
      841 GCACCAGACG GGCGGCGACA TCGAATGGCT GCAGGATTGA AGAGCTTAGA GACCGCTGAG
      901 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
      961 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1021 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1081 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1141 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1201 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1261 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1321 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1381 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1441 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1501 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1561 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1621 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     1681 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     1741 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     1801 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     1861 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     1921 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     1981 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2041 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2101 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2161 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2221 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2281 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2341 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2401 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2461 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2521 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     2581 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     2641 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     2701 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     2761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     2821 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     2881 GGTGGAGTGG GAG
//