LOCUS       Exported     3319 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3319
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1357..1404
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3252..3268
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3269..3300
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3318
                     /label="murC"
                     /note="Cell wall formation."
                     /translation="MTVYHFVGIKGTGMSPLAQILHDNGYTVQGSDIEKFIFTQTALEKRNITILPFSAENIKPGMTVIAGNAFPDTHPEIEKAMSEGIPVIRYHKFLGDYMKKFTSVAVTGAHGKTSTTGLLAHVIQNAKPTSFLIGDGTGQGNENSEYFVFEACEYRRHFLSYQPDYAIMTNIDFDHPDYFSSIDDVFDAFQEMALQVNKGIIACGDDEHLPKIHANVPVVYYGTGEENDFQARNIVKSTEGTTFDVFVRNTFYDTFYIPAYGHHNVLNSLAVIALCHYEEIDSSIIKHALKSFGGVKRRFNEKQLGDQVLIDDYAHHPTEIKVTIEAARQKYPDREIVAVFQPHTFTRTQQFLDEFAESLSGADCVYLCDIFGSARENAGKLTIGDLQGKIHNAKLIEEDDTSVLKAHDKAVLIFMGAGDIQKYMRAYENVMA"
     primer_bind     complement(1409..1425)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1492..2080)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2271..3131)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3132..3236)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG ACCGTGTACC ACTTTGTGGG CATTAAGGGC ACCGGTATGA GCCCGCTGGC
       61 ACAAATTCTG CACGATAACG GCTATACGGT GCAAGGCAGC GACATTGAGA AGTTTATTTT
      121 CACCCAGACC GCCTTGGAAA AACGTAACAT CACCATTCTG CCGTTTTCTG CGGAGAATAT
      181 CAAACCGGGT ATGACCGTTA TCGCGGGTAA TGCGTTTCCG GACACCCACC CGGAGATCGA
      241 GAAAGCAATG AGCGAAGGTA TTCCGGTCAT TCGCTACCAT AAATTCCTGG GTGACTACAT
      301 GAAAAAGTTT ACGAGCGTCG CCGTGACCGG CGCGCACGGT AAAACGAGCA CGACGGGCCT
      361 GCTGGCCCAT GTGATCCAGA ACGCAAAGCC GACCAGCTTC TTGATCGGCG ACGGCACCGG
      421 CCAGGGCAAC GAGAACAGCG AGTACTTTGT GTTTGAGGCG TGTGAATACC GCCGTCATTT
      481 TCTGAGCTAC CAGCCGGACT ATGCCATCAT GACCAACATT GATTTCGATC ACCCTGACTA
      541 CTTTAGCAGC ATTGATGACG TTTTCGATGC ATTTCAGGAG ATGGCGTTAC AGGTCAATAA
      601 GGGCATTATT GCGTGCGGTG ATGACGAACA TCTGCCGAAG ATTCACGCTA ACGTTCCGGT
      661 AGTTTATTAC GGCACCGGTG AAGAAAACGA CTTCCAAGCG CGCAACATCG TTAAGTCGAC
      721 CGAGGGTACC ACCTTCGACG TGTTCGTGCG TAATACCTTT TATGACACCT TCTATATCCC
      781 GGCGTACGGC CACCATAACG TTCTGAACAG CCTGGCAGTT ATCGCGCTGT GCCACTATGA
      841 GGAAATCGAC AGCAGCATCA TCAAGCATGC ATTGAAATCT TTTGGCGGCG TGAAACGCCG
      901 TTTTAATGAA AAGCAACTGG GTGATCAAGT TCTGATTGAC GATTATGCTC ATCACCCGAC
      961 GGAGATTAAA GTGACGATCG AAGCTGCGCG TCAGAAATAT CCGGACCGCG AGATTGTGGC
     1021 CGTGTTCCAG CCGCACACCT TCACGCGTAC TCAGCAGTTT CTGGATGAGT TTGCGGAGTC
     1081 CCTGAGCGGT GCGGATTGCG TGTATCTGTG TGACATTTTC GGTAGCGCGC GTGAGAACGC
     1141 AGGCAAACTG ACCATTGGTG ATCTGCAGGG CAAGATTCAT AACGCCAAAC TCATTGAGGA
     1201 GGATGACACC TCTGTCTTGA AGGCGCACGA CAAAGCCGTT CTGATTTTCA TGGGTGCCGG
     1261 TGATATCCAA AAATATATGC GTGCGTACGA AAACGTTATG GCCTGAAGAG CTTAGAGACC
     1321 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1381 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1441 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1501 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1561 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1621 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1681 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1741 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1801 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1861 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1921 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1981 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2041 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     2101 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     2161 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     2221 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     2281 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2341 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2401 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2461 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2521 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2581 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2641 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2701 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2761 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2821 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     2881 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     2941 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     3001 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     3061 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     3121 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     3181 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     3241 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     3301 TGGTCAGGTG GAGTGGGAG
//