LOCUS       Exported     3922 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3922
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3908
                     /label="asnB"
                     /note="Main asparagine synthetase in vegetative cells."
                     /translation="MCGFVGVFNKHPLAQTADQEELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVENGGQPLSYEDETYWIIFNGEIYNYIELREELEAKGYTFNTDSDTEVLLATYRHYKEEAASKLRGMFAFLIWNKNDHVLYGARDPFGIKPLYYTTINDQVYFASERKSLMVAQNDIEIDKEALQQYMSFQFVPEPSTLDAHVKKVEPGSQFTIRPDGDITFKTYFKANFKPVQTEEDKLVKEVRDAIYDSVNVHMRSDVPVGSFLSGGIDSSFIVSVAKEFHPSLKTFSVGFEQQGFSEVDVAKETAAALGIENISKVISPEEYMNELPKIVWHFDDPLADPAAIPLYFVAKEAKKHVTVALSGEGADELFGGYNIYREPLSLKPFERIPSGLKKMLLHVAAVMPEGMRGKSLLERGCTPLQDRYIGNAKIFEESVKKQLLKHYNPNLSYRDVTKTYFTESSSYSDINKMQYVDIHTWMRGDILLKADKMTMANSLELRVPFLDKVVFDVASKIPDELKTKNGTTKYLLRKAAEGIVPEHVLNRKKLGFPVPIRHWLKNEMNEWVRNIIQESQTDAYIHKDYVLQLLEDHCADKADNSRKIWTVLIFMIWHSINIEKRYMPEELSHQPKEVIFV"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTGCGGCTT TGTTGGCGTT TTTAATAAGC
     2041 ACCCGCTGGC CCAGACCGCC GATCAAGAGG AATTGATTAA GCAGATGAAC CAAATGATCG
     2101 TGCATCGTGG CCCGGATAGC GACGGTTATT TTCATGACGA GCATGTTGGC TTTGGTTTTC
     2161 GTCGCCTGAG CATCATTGAC GTTGAGAACG GCGGCCAACC GCTGAGCTAC GAGGATGAAA
     2221 CCTATTGGAT TATCTTCAAC GGTGAAATCT ACAACTATAT TGAACTGCGC GAGGAGTTGG
     2281 AAGCCAAAGG TTATACCTTT AATACCGACA GCGATACGGA GGTGCTGTTG GCCACCTACC
     2341 GCCACTACAA GGAAGAAGCC GCGTCTAAAC TGCGCGGCAT GTTTGCGTTT CTTATTTGGA
     2401 ACAAAAACGA TCACGTATTG TATGGCGCCC GCGACCCGTT TGGCATTAAG CCGCTGTATT
     2461 ATACCACCAT CAATGATCAA GTGTACTTTG CGAGCGAGCG TAAGAGCCTG ATGGTTGCCC
     2521 AAAACGATAT CGAAATCGAT AAGGAGGCGC TGCAGCAATA CATGAGCTTC CAGTTCGTTC
     2581 CGGAGCCGAG CACTCTGGAT GCACACGTTA AGAAGGTTGA ACCGGGTAGC CAGTTCACGA
     2641 TCCGTCCGGA TGGTGACATC ACCTTTAAGA CCTACTTCAA AGCCAATTTC AAACCGGTTC
     2701 AAACGGAAGA GGACAAACTG GTTAAAGAAG TACGTGATGC AATCTATGAT AGCGTGAACG
     2761 TGCACATGCG TTCCGACGTT CCAGTGGGTA GCTTCCTCAG CGGCGGTATC GACAGCAGCT
     2821 TCATCGTTAG CGTGGCAAAA GAATTCCACC CGTCCTTAAA GACGTTTAGC GTGGGCTTCG
     2881 AACAGCAAGG TTTTAGCGAG GTTGACGTGG CAAAGGAAAC GGCGGCGGCT CTGGGTATTG
     2941 AGAACATTAG CAAAGTGATC AGCCCGGAAG AGTATATGAA TGAGCTGCCG AAAATCGTCT
     3001 GGCACTTCGA TGATCCGCTG GCGGATCCGG CGGCCATCCC GCTGTACTTC GTCGCAAAGG
     3061 AGGCGAAGAA ACACGTGACC GTTGCGCTGT CTGGCGAAGG CGCGGACGAG CTGTTTGGTG
     3121 GTTATAATAT CTATCGCGAA CCGCTGAGCC TGAAACCATT CGAGCGTATC CCGAGCGGTT
     3181 TGAAGAAAAT GCTGCTGCAT GTTGCGGCAG TGATGCCAGA GGGCATGCGT GGCAAGTCCT
     3241 TACTGGAGCG TGGTTGTACG CCGCTGCAAG ATCGCTACAT TGGCAATGCG AAGATTTTTG
     3301 AAGAGAGCGT CAAAAAGCAG CTGCTGAAGC ACTACAATCC AAACCTGAGC TACCGTGATG
     3361 TCACTAAGAC TTACTTTACC GAAAGCTCTA GCTACTCCGA TATTAATAAA ATGCAGTACG
     3421 TTGATATCCA CACTTGGATG CGCGGTGACA TTTTGTTGAA GGCAGACAAG ATGACCATGG
     3481 CGAACAGCCT GGAGCTGCGT GTCCCGTTCC TGGACAAGGT GGTTTTCGAC GTTGCGAGCA
     3541 AAATCCCAGA CGAGCTGAAA ACCAAGAATG GCACCACCAA GTACTTGCTG CGTAAAGCGG
     3601 CCGAAGGTAT TGTTCCGGAG CACGTGCTGA ATCGCAAGAA GCTGGGTTTT CCGGTCCCGA
     3661 TCCGTCATTG GCTCAAGAAT GAAATGAACG AATGGGTTCG CAACATCATT CAGGAAAGCC
     3721 AAACTGATGC GTACATTCAC AAGGATTACG TTCTGCAGCT GCTGGAGGAT CACTGCGCGG
     3781 ATAAAGCGGA CAATAGCCGT AAAATTTGGA CGGTTCTGAT TTTCATGATC TGGCACAGCA
     3841 TTAATATTGA AAAACGCTAT ATGCCGGAGG AGCTGAGCCA CCAACCAAAA GAAGTCATTT
     3901 TCGTGTGAAG AGCTTAGAGA CC
//