LOCUS       Exported     3766 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3766
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3752
                     /label="menD"
                     /note="Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC)."
                     /translation="MTVNPITHYIGSFIDEFALSGITDAVVCPGSRSTPLAVLCAAHPDISVHVQIDERSAGFFALGLAKAKQRPVLLICTSGTAAANFYPAVVEAHYSRVPIIVLTADRPHELREVGAPQAINQHFLFGNFVKFFTDSALPEESPQMLRYIRTLASRAAGEAQKRPMGPVHVNVPLREPLMPDLSDEPFGRMRTGRHVSVKTGTQSVDRESLSDVAEMLAEAEKGMIVCGELHSDADKENIIALSKALQYPILADPLSNLRNGVHDKSTVIDAYDSFLKDDELKRKLRPDVVIRFGPMPVSKPVFLWLKDDPTIQQIVIDEDGGWRDPTQASAHMIHCNASVFAEEIMAGLTAATRSSEWLEKWQFVNGRFREHLQTISSEDVSFEGNLYRILQHLVPENSSLFVGNSMPIRDVDTFFEKQDRPFRIYSNRGANGIDGVVSSAMGVCEGTKAPVTLVIGDLSFYHDLNGLLAAKKLGIPLTVILVNNDGGGIFSFLPQASEKTHFEDLFGTPTGLDFKHAAALYGGTYSCPASWDEFKTAYAPQADKPGLHLIEIKTDRQSRVQLHRDMLNEAVREVKKQWEL"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGACCGTTAA CCCAATCACG CACTACATCG
     2041 GCAGCTTTAT CGATGAGTTT GCCCTGAGCG GCATTACCGA TGCGGTTGTC TGCCCTGGCT
     2101 CTCGTAGCAC CCCGCTGGCT GTGCTGTGTG CGGCGCACCC GGACATTAGC GTTCACGTGC
     2161 AGATTGATGA GCGTAGCGCC GGATTTTTTG CTCTGGGTCT GGCCAAGGCG AAGCAGCGTC
     2221 CAGTTCTGCT GATCTGCACC AGCGGCACCG CCGCTGCTAA CTTCTACCCA GCAGTTGTGG
     2281 AGGCGCACTA CAGCCGCGTC CCGATTATTG TGTTGACGGC CGACCGTCCA CATGAACTGC
     2341 GCGAAGTGGG TGCCCCGCAG GCTATTAACC AGCATTTTTT GTTCGGCAAT TTTGTTAAGT
     2401 TCTTTACCGA CAGCGCACTG CCGGAAGAAT CCCCGCAGAT GCTGCGTTAT ATCCGTACGC
     2461 TGGCGAGCCG TGCTGCGGGC GAGGCACAAA AACGCCCGAT GGGCCCAGTG CATGTGAACG
     2521 TTCCACTGCG TGAGCCGCTG ATGCCGGACT TGAGCGACGA ACCGTTCGGT CGTATGCGCA
     2581 CCGGTCGTCA CGTTAGCGTA AAAACCGGTA CCCAGAGCGT GGATCGTGAA AGCCTGAGCG
     2641 ACGTGGCTGA AATGCTGGCA GAGGCGGAGA AGGGTATGAT TGTGTGCGGC GAACTGCACT
     2701 CAGATGCCGA CAAGGAAAAT ATTATTGCGC TGAGCAAGGC GTTGCAATAT CCAATCCTGG
     2761 CGGACCCGCT GAGCAACTTG CGCAACGGCG TTCACGATAA AAGCACGGTT ATCGATGCGT
     2821 ATGATAGCTT CCTGAAAGAC GACGAGTTGA AGCGCAAACT GCGCCCTGAC GTCGTTATTA
     2881 GATTCGGCCC GATGCCTGTC TCTAAACCGG TGTTCCTGTG GCTGAAGGAT GACCCTACCA
     2941 TTCAACAGAT TGTCATTGAT GAGGATGGTG GTTGGCGCGA CCCGACGCAA GCGAGCGCGC
     3001 ATATGATCCA CTGTAACGCA AGCGTTTTCG CGGAAGAGAT CATGGCGGGT CTGACTGCAG
     3061 CGACCCGTAG CAGCGAGTGG CTGGAAAAGT GGCAGTTCGT CAACGGTCGC TTTCGTGAAC
     3121 ATCTGCAAAC TATCAGCAGC GAGGACGTGA GCTTCGAGGG TAACCTGTAT CGCATCCTGC
     3181 AGCACCTGGT TCCGGAGAAT TCCAGCCTGT TTGTCGGTAA TTCTATGCCA ATCCGTGACG
     3241 TTGACACCTT CTTTGAGAAG CAGGATCGTC CGTTCCGTAT TTACTCTAAC CGTGGTGCGA
     3301 ACGGTATTGA TGGTGTGGTT AGCTCGGCCA TGGGCGTTTG CGAAGGCACG AAGGCGCCGG
     3361 TCACCCTGGT CATCGGCGAT CTGTCCTTCT ACCACGACCT GAACGGTCTG CTGGCGGCAA
     3421 AGAAGCTGGG TATCCCGCTG ACGGTCATTC TGGTCAATAA CGATGGTGGT GGTATCTTTT
     3481 CCTTTCTGCC GCAAGCCAGC GAAAAGACCC ACTTTGAAGA TCTGTTCGGT ACCCCTACCG
     3541 GCCTGGATTT CAAACATGCT GCCGCGTTGT ACGGTGGTAC TTACAGCTGC CCGGCGAGCT
     3601 GGGACGAGTT CAAGACCGCA TACGCACCGC AAGCGGACAA ACCGGGCCTG CACCTGATTG
     3661 AAATCAAAAC CGACCGCCAA AGCCGCGTTC AGCTGCACCG CGATATGTTG AACGAGGCCG
     3721 TCCGCGAGGT TAAAAAACAG TGGGAGCTGT GAAGAGCTTA GAGACC
//