LOCUS       Exported     4429 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4429
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4415
                     /label="mrpA"
                     /note="Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+)."
                     /translation="MQLLHLAILSPFLFAFIIPFLAKYAKRVHTGWFVLILPVLLFIYFLPMIRMTQSGETLRSVLEWIPSLGINFTVYIDGLGLLFALLITGIGSLVTLYSIFYLSKEKEQLGPFYVYLLMFMGAMLGVVLVDNVMVLYMFWELTSLSSFLLIGYWYKREKSRYGAAKSLLITVSGGLCMLGGFILLYLITDSFSIREMVHQVQLIAGHELFIPAMILILLGAFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGIYVIARFSPIFAFSAQWFWIVSLVGLFTMVWGSFHAVKQTDLKSILAFSTVSQLGMIISMLGVSAAALHYGHTEYYTVAAMAAIFHLINHATFKGSLFMAVGIIDHETGTRDIRKLGGLMAIMPITFTISLIGTFSMAGLPPFNGFLSKEMFFTSMLRVTHFDLFNVQTWGVLFPLFAWIGSVFTFIYSMKLLFKTFRGNYQPEQLEKQAHEAPVGMLVPPVILVALAVSLFFFPNILSYSLIEPAMNSIYPTLLDGHEKFHVHISQWHGVTTELLMTAGIVVIGTIGYLSLNKWKGIYKLFPSKLTLNRLYDKLLTMMEKGSYRVTKQYMTGFLRDYLLYIFAGFIILIGGAFAIKGGFSFKTEGMAKIGVYEIILTLVMISATVATVFARSRLTAIIALGVVGYTLALFFVIFRAPDLALTQLVIETISVALFLLCFYHLPKLRLKTKTRTFRMTNFIISLGVGVIVTLLGIASSSQRTKDSIASFFVKHSHDLGGGDNVVNVILVDFRGFDTMFEITVLTIAALGIYSMIKTKVKEEGKSGE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCAACTGCT GCACCTGGCG ATTCTGTCTC
     2041 CATTTCTGTT CGCGTTTATT ATCCCGTTCC TGGCAAAATA TGCGAAGCGT GTTCACACCG
     2101 GTTGGTTCGT GCTGATTCTG CCGGTTCTGC TGTTTATTTA CTTTCTGCCG ATGATTCGCA
     2161 TGACCCAGAG CGGTGAAACC CTGCGCTCGG TTCTGGAGTG GATCCCGAGC TTGGGTATTA
     2221 ACTTTACGGT CTACATTGAT GGTCTTGGTC TGCTGTTTGC TCTGCTGATC ACGGGTATTG
     2281 GTAGCCTGGT CACTCTGTAC TCTATCTTCT ACCTGAGCAA GGAGAAAGAG CAACTGGGCC
     2341 CGTTTTACGT TTACTTGTTG ATGTTCATGG GCGCCATGCT GGGTGTTGTT CTGGTTGATA
     2401 ATGTCATGGT GCTGTACATG TTTTGGGAAC TGACCAGCCT GAGCAGCTTT CTGCTGATTG
     2461 GTTACTGGTA TAAACGCGAA AAGAGCCGTT ATGGTGCCGC AAAAAGCCTG CTGATCACCG
     2521 TCAGCGGCGG TCTGTGCATG CTGGGCGGCT TTATTCTGCT TTATCTGATC ACGGATAGCT
     2581 TCTCTATTCG TGAGATGGTG CATCAAGTTC AGCTTATCGC CGGCCATGAG CTGTTCATTC
     2641 CAGCAATGAT TCTGATTCTG CTGGGTGCGT TTACCAAGAG CGCACAGTTT CCGTTCTACA
     2701 TTTGGCTTCC GGATGCCATG GAAGCGCCGA CCCCGGTCAG CGCCTATCTG CACTCCGCGA
     2761 CCATGGTAAA AGCCGGCATT TACGTAATCG CGCGTTTTTC CCCGATTTTT GCGTTTTCCG
     2821 CACAATGGTT TTGGATTGTG TCGTTGGTCG GTTTATTCAC CATGGTCTGG GGCTCTTTTC
     2881 ATGCGGTCAA GCAAACGGAC CTGAAATCAA TCCTGGCGTT TAGCACGGTG TCGCAGCTGG
     2941 GTATGATTAT CAGCATGCTG GGTGTGAGCG CGGCTGCGCT GCACTATGGT CACACCGAGT
     3001 ACTACACTGT AGCAGCAATG GCCGCCATCT TTCACCTGAT CAATCACGCG ACGTTTAAGG
     3061 GTTCACTGTT CATGGCCGTT GGCATCATCG ACCACGAAAC CGGCACCCGC GACATCCGTA
     3121 AACTGGGCGG CCTGATGGCG ATTATGCCGA TCACGTTTAC CATCAGCCTG ATTGGCACGT
     3181 TCAGCATGGC CGGTCTGCCG CCGTTCAATG GCTTTCTGAG CAAAGAGATG TTCTTCACCT
     3241 CTATGCTGCG TGTCACCCAC TTCGACCTGT TTAACGTTCA AACCTGGGGC GTTTTGTTCC
     3301 CGCTGTTTGC GTGGATTGGC AGCGTTTTTA CGTTCATTTA CAGCATGAAA TTACTGTTCA
     3361 AAACCTTCAG AGGTAACTAC CAACCGGAAC AGCTGGAAAA ACAAGCTCAT GAGGCGCCGG
     3421 TTGGTATGCT GGTCCCGCCG GTTATTCTGG TTGCACTTGC GGTTAGCCTG TTCTTTTTTC
     3481 CGAACATTCT GAGCTATTCT CTGATTGAAC CAGCCATGAA CTCCATCTAC CCGACCTTGC
     3541 TGGATGGCCA CGAGAAATTT CACGTTCACA TTAGCCAATG GCATGGTGTG ACCACGGAGC
     3601 TGCTGATGAC GGCGGGTATC GTGGTGATCG GCACTATCGG TTATTTGTCC CTGAACAAGT
     3661 GGAAAGGTAT CTATAAGCTG TTTCCGAGCA AGCTGACCCT GAACCGTCTG TACGACAAGC
     3721 TTCTGACGAT GATGGAGAAG GGCAGCTATC GTGTGACCAA ACAGTATATG ACTGGTTTCC
     3781 TCCGTGATTA TCTGCTGTAT ATTTTCGCTG GTTTTATTAT CCTGATTGGC GGTGCGTTCG
     3841 CGATTAAGGG TGGTTTTAGC TTCAAGACGG AGGGTATGGC CAAAATTGGT GTGTATGAGA
     3901 TCATTCTGAC CCTGGTTATG ATTAGCGCGA CCGTGGCGAC CGTGTTCGCG CGCTCCCGTC
     3961 TGACGGCAAT TATTGCTCTG GGTGTTGTCG GTTACACCTT GGCACTGTTC TTTGTGATTT
     4021 TTCGCGCTCC AGACCTGGCA CTGACCCAAC TGGTGATCGA AACCATTTCG GTCGCGTTGT
     4081 TCCTGCTGTG TTTTTATCAT CTGCCGAAGC TGCGCCTCAA GACGAAAACG CGTACCTTCC
     4141 GTATGACCAA TTTCATTATT TCTCTGGGTG TTGGTGTGAT CGTCACCTTG CTGGGGATCG
     4201 CGAGCAGCAG CCAGCGCACC AAGGACAGCA TTGCGAGCTT TTTCGTCAAG CATAGCCACG
     4261 ATCTGGGTGG TGGCGACAAC GTGGTCAACG TTATCCTTGT TGACTTTCGT GGTTTCGATA
     4321 CCATGTTCGA AATTACGGTT CTGACCATTG CAGCCTTGGG CATCTACTCT ATGATTAAGA
     4381 CGAAGGTAAA AGAAGAGGGC AAAAGCGGCG AGTGAAGAGC TTAGAGACC
//