LOCUS       Exported     3505 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3505
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3491
                     /label="mrpD"
                     /note="Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+)."
                     /translation="MNNFVILPILIPLLSAILLIFMTKNLMLMRIFSTAASAIGIVISGILVQTVFTKGIQTLSLGGWKAPYGIVLAADQFASLLVLTTAIIGLLVGLYSFRSVGEKRERSFYYSGVQFLLAGVSGAFLTGDLFNMYVFFELLLIASYMLIVLGGTKIQLRESLKYIVFNIVSSALFVIGVGFLYAVTGTLNMADLSVKISESGQTGLITVIGVLLLLVFGMKGGIFPLYFWLPGSYYAPPAAISALFGALLTKVGLYAITRVFTLIFIHDTAFTHQLMIWLAALTVIFGVIGSLAYSNVMKIVIYNIITAVGVILFGVAVHTPASIQGAIYYLIHDMLIKGALFMLAGTLIALTGTASLHKMGGLIKRYPVLGWMFFISAISLAGIPPLSGFVGKFKIAEGGFAEGEFTISMLILLSSLLVLYSVLRIFIHAFWGEEKETPKPNHRTAKGLLYPAAIFLLLSLLFGLGTEWVSPYVDQAAETLLNPEKYIEAVLKE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAATAACTT TGTCATTCTG CCGATTTTGA
     2041 TTCCGCTGCT TAGCGCAATT CTGCTGATCT TTATGACCAA AAACTTGATG CTCATGCGCA
     2101 TCTTTTCGAC GGCGGCGTCG GCGATCGGCA TCGTTATCTC CGGCATTCTG GTCCAGACCG
     2161 TCTTTACCAA GGGCATTCAG ACGCTGAGCT TGGGTGGTTG GAAAGCCCCG TACGGCATCG
     2221 TGCTGGCGGC GGATCAGTTC GCGAGCCTGT TGGTTCTGAC CACCGCCATC ATTGGCTTGC
     2281 TGGTCGGTTT ATACTCTTTC CGCAGCGTGG GTGAGAAGCG TGAACGTAGC TTCTATTACA
     2341 GCGGCGTGCA ATTCCTGCTG GCGGGTGTCT CTGGCGCATT TTTGACTGGT GACTTATTCA
     2401 ACATGTATGT GTTTTTTGAG CTGCTGCTGA TTGCGAGCTA TATGCTGATC GTGCTGGGTG
     2461 GTACGAAAAT CCAACTGCGT GAAAGCTTGA AATACATTGT CTTTAATATC GTTTCCAGCG
     2521 CTCTGTTCGT CATTGGTGTT GGCTTTCTGT ACGCGGTTAC GGGTACCCTG AACATGGCGG
     2581 ACCTGAGCGT CAAAATCTCC GAGTCCGGCC AAACGGGTTT GATTACCGTA ATCGGTGTTC
     2641 TGCTGCTGCT GGTTTTCGGC ATGAAAGGTG GCATTTTTCC GCTGTATTTC TGGCTGCCGG
     2701 GTAGCTACTA CGCACCGCCG GCCGCGATTA GCGCGCTGTT TGGCGCGCTG CTGACCAAGG
     2761 TCGGCCTGTA CGCGATTACG CGTGTCTTTA CGCTGATCTT CATTCACGAC ACCGCATTCA
     2821 CCCATCAATT GATGATTTGG CTGGCGGCAT TGACCGTGAT TTTTGGCGTT ATCGGTAGCT
     2881 TGGCATACAG CAATGTTATG AAAATTGTTA TTTATAATAT CATCACGGCA GTGGGTGTGA
     2941 TCCTGTTCGG TGTCGCGGTT CATACCCCAG CGAGCATCCA GGGTGCGATC TACTACTTGA
     3001 TCCACGATAT GCTGATTAAA GGTGCCCTGT TCATGCTGGC CGGTACCCTT ATCGCGCTGA
     3061 CGGGCACCGC CTCTCTGCAC AAGATGGGCG GTCTGATCAA GCGTTACCCG GTTCTGGGTT
     3121 GGATGTTTTT TATTAGCGCG ATTAGCCTGG CTGGTATTCC GCCGCTGAGC GGCTTCGTGG
     3181 GCAAATTCAA AATCGCGGAG GGCGGCTTCG CGGAGGGTGA GTTTACGATT AGCATGCTGA
     3241 TTCTGCTGAG CAGCCTGCTG GTGCTGTACA GCGTTCTGCG TATTTTTATT CATGCTTTCT
     3301 GGGGTGAGGA GAAGGAAACG CCAAAACCGA ACCACCGCAC CGCCAAAGGT CTGCTCTATC
     3361 CGGCGGCTAT CTTTCTGCTG TTGAGCCTGC TGTTCGGCTT GGGTACGGAG TGGGTCAGCC
     3421 CGTACGTGGA CCAAGCGGCG GAAACCTTGC TGAACCCTGA AAAGTATATT GAAGCAGTTC
     3481 TTAAGGAGTG AAGAGCTTAG AGACC
//