LOCUS Exported 3337 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3337 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 2010..3323 /label="sufD" /note="The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation (By similarity)." /translation="MTLGTKLSVDQEYLKSFSEKHQEPAWLKNLRLQALEQAEDLPMPKPDKTKITNWNFTNFAKHTVDNEPLSSLEDLTDEVKALIDIENEDKTLYVQRDQTPAHLSLSQELKDKGVIFTDILTAAREHSDLVEKYFMKDGVKVDEHKLTALHAALVNGGAFLYVPKNVQVETPVQAVYVHESNDTALFNHVLIVAEDHSSVTYVENYISTVNPKDAVFNIISEVITGDNASVTYGAVDNLSSGVTTYVNRRGAARGRDSKIEWALGLMNDGDTISENTTNLYGDGTYGDTKTVVVGRGEQTENFTTQIIHFGKASEGYILKHGVMKDSASSIFNGIGKIEHGASKANAEQESRVLMLSEKARGDANPILLIDEDDVTAGHAASVGRVDPIQLYYLMSRGIPKEEAERLVIYGFLAPVVNELPIEGVKKQLVSVIERKVK" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGACGCTGGG CACGAAACTG TCCGTTGACC 2041 AGGAATATCT GAAAAGCTTT TCGGAAAAGC ACCAGGAACC GGCTTGGCTG AAAAATCTGC 2101 GTCTGCAGGC ATTGGAGCAA GCGGAAGATT TGCCGATGCC GAAACCGGAC AAAACCAAAA 2161 TTACGAATTG GAATTTCACC AATTTTGCGA AACACACTGT GGATAACGAG CCGCTGTCCA 2221 GCTTGGAGGA TCTGACCGAT GAGGTTAAAG CGTTGATCGA TATTGAAAAT GAGGATAAGA 2281 CGCTGTATGT GCAGCGTGAT CAGACCCCGG CACATCTGAG CCTGAGCCAG GAACTGAAGG 2341 ACAAGGGCGT TATCTTCACG GATATTCTGA CCGCGGCACG TGAGCACAGC GATCTGGTGG 2401 AAAAATATTT CATGAAAGAT GGTGTTAAAG TTGACGAACA CAAACTGACC GCGCTGCATG 2461 CAGCATTGGT TAACGGTGGT GCGTTCCTGT ATGTTCCGAA GAATGTCCAA GTTGAAACGC 2521 CGGTGCAAGC AGTTTACGTC CATGAGTCCA ACGATACTGC GCTGTTTAAC CACGTCCTGA 2581 TCGTGGCCGA GGACCATAGC AGCGTTACCT ACGTTGAGAA CTACATTAGC ACGGTTAATC 2641 CGAAGGACGC GGTTTTTAAC ATCATTTCTG AGGTTATTAC CGGTGACAAC GCATCTGTCA 2701 CCTACGGCGC AGTTGATAAC TTGAGCTCTG GTGTGACCAC CTACGTCAAC CGTCGCGGTG 2761 CGGCACGCGG CCGTGATAGC AAGATTGAAT GGGCGCTGGG CTTGATGAAC GACGGTGATA 2821 CTATCAGCGA GAACACCACG AATCTGTATG GAGATGGCAC CTATGGTGAC ACCAAGACCG 2881 TCGTGGTCGG TCGTGGCGAA CAGACCGAGA ACTTCACGAC GCAAATCATC CATTTTGGCA 2941 AGGCGAGCGA GGGTTACATT CTGAAGCACG GCGTCATGAA AGACAGCGCA TCCAGCATCT 3001 TTAATGGTAT TGGCAAGATT GAACACGGTG CGAGCAAAGC AAATGCAGAG CAAGAGAGCC 3061 GTGTGCTGAT GCTGTCCGAA AAAGCCCGCG GCGACGCGAA CCCGATTTTG CTGATCGATG 3121 AGGACGACGT TACGGCGGGT CACGCAGCAA GCGTGGGTCG TGTTGACCCG ATCCAATTGT 3181 ACTACCTGAT GAGCCGTGGT ATCCCAAAGG AAGAAGCTGA ACGCTTAGTT ATTTATGGCT 3241 TCCTGGCCCC GGTGGTGAAC GAGCTGCCGA TCGAGGGCGT CAAGAAGCAG CTGGTTAGCG 3301 TCATCGAACG CAAAGTGAAG TGAAGAGCTT AGAGACC //