LOCUS       Exported     3313 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3313
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1351..1398
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3246..3262
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3263..3294
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3312
                     /label="eno"
                     /note="Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis."
                     /translation="MPYIVDVYAREVLDSRGNPTVEVEVYTETGAFGRALVPSGASTGEYEAVELRDGDKDRYLGKGVLTAVNNVNEIIAPELLGFDVTEQNAIDQLLIELDGTENKGKLGANAILGVSMACARAAADFLQIPLYQYLGGFNSKTLPVPMMNIVNGGEHADNNVDIQEFMIMPVGAPNFREALRMGAQIFHSLKSVLSAKGLNTAVGDEGGFAPNLGSNEEALQTIVEAIEKAGFKPGEEVKLAMDAASSEFYNKEDGKYHLSGEGVVKTSAEMVDWYEELVSKYPIISIEDGLDENDWEGHKLLTERLGKKVQLVGDDLFVTNTKKLSEGIKNGVGNSILIKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDQLAETAQYHGINSFYNLNK"
     primer_bind     complement(1403..1419)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1486..2074)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2265..3125)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3126..3230)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 GTCTCAAATG CCGTACATTG TCGACGTTTA CGCTCGTGAG GTGCTGGATA GCCGCGGCAA
       61 CCCAACGGTC GAGGTGGAGG TCTACACGGA AACCGGCGCA TTCGGCCGTG CGCTGGTTCC
      121 GTCCGGTGCC AGCACCGGCG AGTACGAGGC GGTCGAACTT CGCGACGGTG ATAAAGACAG
      181 ATACCTGGGT AAGGGCGTGC TGACGGCTGT GAACAATGTT AACGAGATCA TTGCCCCGGA
      241 GCTGCTGGGC TTTGACGTCA CCGAGCAAAA TGCGATTGAT CAGCTGCTGA TTGAGCTGGA
      301 TGGCACGGAG AACAAGGGTA AGCTGGGCGC AAACGCTATT CTGGGCGTTT CAATGGCGTG
      361 TGCGCGTGCG GCGGCAGACT TTCTGCAAAT CCCGCTGTAT CAGTACTTGG GTGGCTTCAA
      421 TAGCAAGACG TTGCCAGTGC CAATGATGAA CATCGTTAAT GGTGGCGAAC ACGCTGATAA
      481 TAACGTTGAC ATTCAAGAGT TTATGATCAT GCCGGTCGGT GCTCCGAACT TTCGCGAGGC
      541 GCTGCGTATG GGCGCACAGA TCTTTCACTC CTTGAAGTCC GTCCTGAGCG CTAAAGGTCT
      601 GAACACGGCG GTCGGTGACG AAGGTGGTTT CGCGCCGAAC CTGGGTAGCA ATGAAGAGGC
      661 GCTGCAAACG ATCGTCGAGG CGATTGAGAA AGCGGGTTTT AAGCCGGGTG AAGAAGTCAA
      721 ACTGGCCATG GACGCCGCTT CTAGCGAATT CTACAATAAA GAGGACGGCA AATATCACCT
      781 GAGCGGTGAA GGTGTTGTCA AAACGTCCGC GGAGATGGTT GATTGGTATG AAGAGTTAGT
      841 CTCTAAGTAC CCGATCATCA GCATCGAGGA TGGCCTGGAT GAGAATGATT GGGAAGGCCA
      901 CAAACTGCTG ACGGAGCGTC TGGGCAAAAA AGTCCAGCTG GTGGGCGACG ACTTGTTCGT
      961 CACGAATACG AAAAAACTGA GCGAAGGTAT TAAAAATGGC GTGGGTAATA GCATCCTGAT
     1021 CAAGGTCAAC CAGATTGGCA CGCTGACCGA AACGTTTGAT GCTATTGAGA TGGCAAAACG
     1081 TGCGGGCTAC ACGGCTGTGA TCAGCCATCG TAGCGGCGAA ACCGAGGACT CCACTATCGC
     1141 CGATATCGCA GTAGCGACCA ATGCAGGCCA GATTAAGACC GGTGCACCTA GCCGTACCGA
     1201 TCGTGTGGCC AAATATAATC AGCTGCTGCG TATTGAGGAC CAACTGGCAG AAACCGCCCA
     1261 GTATCACGGT ATCAACTCTT TTTATAACCT GAACAAGTGA AGAGCTTAGA GACCGCTGAG
     1321 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1381 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1441 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1501 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1561 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1621 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1681 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1741 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1801 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1861 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1921 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1981 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2041 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2101 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2161 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2221 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2281 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2341 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2401 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2461 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2521 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2581 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2641 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2701 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2761 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2821 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2881 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2941 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     3001 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3061 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3121 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3181 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3241 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     3301 GGTGGAGTGG GAG
//