LOCUS       Exported     3556 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3556
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1594..1641
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3489..3505
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3506..3537
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3555
                     /label="pgm"
                     /note="Essential for rapid growth and for sporulation. Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate."
                     /translation="MSKKPAALIILDGFGLRNETVGNAVALAKKPNFDRYWNQYPHQTLTASGEAVGLPEGQMGNSEVGHLNIGAGRIVYQSLTRVNVAIREGEFERNQTFLDAISNAKENNKALHLFGLLSDGGVHSHINHLFALLKLAKKEGLTKVYIHGFLDGRDVGPQTAKTYINQLNDQIKEIGVGEIASISGRYYSMDRDKRWDRVEKAYRAMAYGEGPSYRSALDVVDDSYANGIYDEFVIPSVITKENGEPVAKIQDGDSVIFYNFRPDRAIQISNTFTNKDFRDFDRGENYPKNLYFVCLTHFSETVDGYVAFKPINLDNTVGEVLSQHGLKQLRIAETEKYPHVTFFMSGGREAEFPGEERILINSPKVATYDLKPEMSAYEVKDALVKEIEADKHDAIILNFANPDMVGHSGMVEPTIKAIEAVDECLGEVVDAILAKGGHAIITADHGNADILITESGEPHTAHTTNPVPVIVTKEGITLREGGILGDLAPTLLDLLGVEKPKEMTGTSLIQK"
     primer_bind     complement(1646..1662)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1729..2317)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2508..3368)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3369..3473)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG TCCAAAAAGC CGGCAGCACT GATCATCCTG GACGGCTTCG GTCTGCGTAA
       61 CGAAACGGTC GGTAACGCCG TCGCCCTGGC CAAAAAGCCA AATTTCGATC GCTACTGGAA
      121 TCAATACCCG CACCAGACCT TGACCGCGAG CGGTGAGGCG GTAGGCCTGC CGGAAGGCCA
      181 GATGGGCAAC AGCGAAGTGG GCCACTTGAA TATTGGCGCG GGTCGCATCG TGTACCAGAG
      241 CCTGACTCGT GTGAACGTCG CGATCCGTGA AGGTGAATTC GAGCGTAACC AGACCTTCCT
      301 GGACGCAATC AGCAACGCGA AAGAGAATAA CAAAGCCCTG CACTTATTCG GCCTGTTGAG
      361 CGACGGCGGT GTTCACAGCC ATATTAACCA CCTTTTCGCG CTGCTTAAGC TGGCCAAAAA
      421 GGAGGGCCTG ACTAAAGTTT ATATCCACGG TTTCTTAGAC GGTCGCGACG TCGGCCCGCA
      481 GACGGCCAAG ACGTACATTA ACCAGCTGAA TGATCAGATT AAAGAGATTG GTGTCGGCGA
      541 GATTGCCAGC ATTAGCGGTC GCTATTATAG CATGGATCGT GACAAGCGCT GGGACCGTGT
      601 AGAGAAGGCA TATCGCGCCA TGGCTTACGG CGAAGGTCCG TCCTACCGTA GCGCGCTGGA
      661 CGTGGTGGAC GACAGCTACG CCAATGGCAT TTATGACGAG TTTGTCATCC CGAGCGTCAT
      721 CACCAAGGAG AATGGTGAGC CGGTCGCGAA GATCCAAGAC GGTGATAGCG TTATTTTCTA
      781 TAACTTCCGT CCGGATCGTG CGATTCAAAT TAGCAATACG TTCACCAATA AGGATTTCCG
      841 TGACTTCGAC CGCGGCGAAA ATTACCCGAA GAATTTGTAC TTCGTGTGCT TGACTCACTT
      901 CAGCGAAACC GTGGACGGCT ACGTGGCATT CAAACCGATC AATCTGGATA ACACCGTTGG
      961 TGAGGTGCTG AGCCAGCACG GCTTGAAGCA GCTGCGTATT GCAGAAACGG AAAAGTACCC
     1021 GCATGTCACT TTCTTCATGA GCGGTGGCAG AGAGGCAGAG TTCCCGGGTG AGGAGCGTAT
     1081 CCTGATTAAT AGCCCGAAAG TGGCAACGTA CGATCTGAAA CCGGAGATGT CGGCGTACGA
     1141 GGTCAAAGAC GCGCTGGTTA AAGAAATTGA AGCTGACAAG CACGACGCCA TTATCCTGAA
     1201 TTTCGCAAAT CCGGACATGG TCGGTCACAG CGGTATGGTG GAACCGACCA TTAAAGCGAT
     1261 CGAGGCTGTC GACGAATGCC TGGGTGAAGT CGTCGATGCA ATTCTGGCGA AGGGTGGCCA
     1321 TGCGATCATC ACCGCGGATC ATGGTAACGC GGATATTCTG ATTACCGAGT CGGGCGAACC
     1381 GCACACCGCT CACACGACTA ACCCAGTGCC GGTTATCGTT ACGAAGGAGG GCATCACGCT
     1441 GCGCGAGGGT GGTATCCTGG GTGATCTCGC TCCGACGCTG CTGGATCTGC TGGGCGTGGA
     1501 GAAACCTAAG GAAATGACTG GCACGAGCCT GATCCAGAAG TGAAGAGCTT AGAGACCGCT
     1561 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1621 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1681 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1741 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1801 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1861 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1921 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1981 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     2041 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     2101 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2161 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2221 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2281 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2341 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2401 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2461 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2521 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2581 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2641 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2701 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2761 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2821 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2881 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2941 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     3001 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     3061 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3121 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3181 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3241 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3301 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3361 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3421 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3481 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3541 TCAGGTGGAG TGGGAG
//