LOCUS       Exported     4549 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4549
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4535
                     /translation="MLGILNKMFDPTKRTLNRYEKIANDIDAIRGDYENLSDDALKHKTIEFKERLEKGATTDDLLVEAFAVVREASRRVTGMFPFKVQLMGGVALHDGNIAEMKTGEGKTLTSTLPVYLNALTGKGVHVVTVNEYLASRDAEQMGKIFEFLGLTVGLNLNSMSKDEKREAYAADITYSTNNELGFDYLRDNMVLYKEQMVQRPLHFAVIDEVDSILIDEARTPLIISGQAAKSTKLYVQANAFVRTLKAEKDYTYDIKTKAVQLTEEGMTKAEKAFGIDNLFDVKHVALNHHINQALKAHVAMQKDVDYVVEDGQVVIVDSFTGRLMKGRRYSEGLHQAIEAKEGLEIQNESMTLATITFQNYFRMYEKLAGMTGTAKTEEEEFRNIYNMQVVTIPTNRPVVRDDRPDLIYRTMEGKFKAVAEDVAQRYMTGQPVLVGTVAVETSELISKLLKNKGIPHQVLNAKNHEREAQIIEEAGQKGAVTIATNMAGRGTDIKLGEGVKELGGLAVVGTERHESRRIDNQLRGRSGRQGDPGITQFYLSMEDELMRRFGAERTMAMLDRFGMDDSTPIQSKMVSRAVESSQKRVEGNNFDSRKQLLQYDDVLRQQREVIYKQRFEVIDSENLREIVENMIKSSLERAIAAYTPREELPEEWKLDGLVDLINTTYLDEGALEKSDIFGKEPDEMLELIMDRIITKYNEKEEQFGKEQMREFEKVIVLRAVDSKWMDHIDAMDQLRQGIHLRAYAQTNPLREYQMEGFAMFEHMIESIEDEVAKFVMKAEIENNLEREEVVQGQTTAHQPQEGDDNKKAKKAPVRKVVDIGRNAPCHCGSGKKYKNCCGRTE"
                     /label="secA"
                     /note="Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCTGGGTAT CCTGAATAAG ATGTTTGATC
     2041 CGACCAAACG CACCTTGAAC CGCTACGAGA AGATTGCAAA TGACATTGAC GCTATTCGCG
     2101 GCGATTACGA AAACCTGTCC GATGACGCGC TGAAACACAA GACTATCGAG TTCAAAGAAC
     2161 GTTTAGAGAA AGGCGCGACG ACGGATGATC TGCTGGTCGA GGCTTTTGCA GTGGTTCGTG
     2221 AGGCTAGCCG TCGTGTGACC GGTATGTTCC CGTTTAAAGT CCAGCTGATG GGCGGTGTCG
     2281 CACTGCACGA CGGTAACATT GCAGAGATGA AAACCGGTGA AGGCAAAACC CTGACGTCCA
     2341 CGCTGCCGGT GTACCTGAAT GCACTGACCG GTAAGGGTGT TCATGTTGTC ACCGTGAACG
     2401 AATACCTGGC CAGCCGTGAT GCCGAGCAAA TGGGTAAAAT TTTCGAGTTC TTAGGTCTGA
     2461 CGGTCGGTCT GAACCTGAAC TCTATGAGCA AGGACGAGAA GCGCGAGGCG TACGCGGCCG
     2521 ATATCACCTA CAGCACGAAT AACGAGCTGG GCTTTGATTA CCTCCGTGAC AACATGGTCC
     2581 TGTACAAGGA ACAAATGGTG CAGCGCCCGC TGCACTTTGC CGTCATCGAC GAAGTGGACT
     2641 CCATTCTGAT TGATGAGGCA CGTACCCCGC TGATCATCAG CGGTCAGGCG GCTAAGAGCA
     2701 CTAAGCTGTA CGTCCAGGCA AACGCTTTCG TCCGCACGCT GAAGGCCGAA AAGGATTACA
     2761 CCTACGATAT CAAAACCAAA GCCGTGCAGC TGACCGAGGA GGGTATGACG AAAGCCGAAA
     2821 AAGCCTTCGG TATCGACAAT TTGTTTGACG TTAAGCATGT TGCACTGAAT CACCACATCA
     2881 ACCAGGCGTT GAAAGCACAT GTGGCCATGC AGAAGGATGT GGACTACGTC GTTGAGGACG
     2941 GTCAAGTCGT CATTGTCGAT TCCTTCACCG GCCGTTTGAT GAAGGGTCGC CGTTATTCCG
     3001 AGGGCCTCCA CCAAGCTATC GAAGCTAAAG AGGGTCTGGA AATCCAAAAT GAGAGCATGA
     3061 CGCTGGCGAC TATCACGTTT CAAAACTATT TCCGTATGTA TGAGAAACTG GCGGGCATGA
     3121 CCGGCACGGC CAAAACCGAG GAGGAGGAAT TCCGTAACAT CTACAATATG CAAGTGGTGA
     3181 CCATTCCGAC CAACCGCCCA GTGGTGCGTG ATGATCGTCC GGATCTGATC TACCGTACCA
     3241 TGGAGGGTAA GTTTAAGGCA GTGGCGGAGG ACGTGGCACA GCGTTATATG ACCGGTCAGC
     3301 CGGTGCTGGT GGGTACCGTC GCCGTTGAAA CGTCTGAATT GATTAGCAAA CTGCTGAAGA
     3361 ACAAAGGCAT CCCGCACCAG GTCCTGAATG CGAAAAATCA TGAACGTGAA GCACAGATTA
     3421 TTGAAGAGGC GGGTCAAAAA GGCGCTGTTA CGATTGCAAC CAACATGGCG GGTCGTGGTA
     3481 CCGATATCAA GCTGGGTGAG GGTGTCAAGG AGTTGGGTGG TCTGGCAGTT GTTGGTACCG
     3541 AGCGTCACGA ATCCCGCCGT ATTGATAACC AACTGCGTGG TCGTAGCGGT CGCCAAGGCG
     3601 ACCCGGGCAT CACCCAGTTT TACCTGAGCA TGGAGGACGA GCTGATGCGC CGCTTCGGTG
     3661 CAGAACGTAC GATGGCCATG CTGGATCGTT TCGGCATGGA CGATAGCACC CCTATCCAAA
     3721 GCAAGATGGT GAGCCGTGCC GTCGAGAGCT CCCAGAAACG TGTCGAAGGT AACAATTTCG
     3781 ATTCTCGTAA GCAACTGTTG CAGTACGACG ACGTCCTGCG TCAGCAACGT GAAGTGATTT
     3841 ACAAACAGCG CTTTGAGGTG ATTGACAGCG AGAACCTCCG CGAAATCGTC GAAAATATGA
     3901 TCAAATCTAG CCTCGAGCGT GCGATTGCAG CTTATACCCC GCGTGAGGAG CTGCCGGAGG
     3961 AATGGAAGCT GGATGGTCTG GTCGATCTGA TTAACACCAC CTACTTAGAT GAAGGCGCCC
     4021 TGGAGAAAAG CGACATTTTT GGCAAAGAGC CGGACGAGAT GCTGGAACTG ATCATGGATC
     4081 GTATCATTAC TAAGTATAAT GAGAAGGAAG AACAATTTGG TAAAGAACAG ATGCGCGAGT
     4141 TCGAAAAAGT GATTGTTCTG CGTGCGGTCG ATAGCAAATG GATGGACCAT ATCGATGCAA
     4201 TGGACCAGTT GCGCCAAGGC ATCCATCTGC GCGCATACGC GCAAACCAAC CCGCTGCGTG
     4261 AATACCAAAT GGAGGGCTTT GCCATGTTTG AGCACATGAT CGAGAGCATT GAGGATGAGG
     4321 TCGCGAAGTT TGTTATGAAA GCAGAGATCG AGAATAACCT GGAGCGCGAA GAAGTCGTCC
     4381 AAGGTCAAAC GACTGCACAC CAACCGCAAG AGGGTGATGA TAATAAAAAA GCTAAAAAAG
     4441 CGCCGGTTCG CAAAGTGGTC GATATCGGCC GTAACGCGCC GTGTCATTGC GGCAGCGGTA
     4501 AGAAATATAA GAACTGTTGC GGTCGCACTG AGTGAAGAGC TTAGAGACC
//