LOCUS       Exported     3475 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3475
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3461
                     /translation="MPSITKQIMSGAKWTSISTMCITIIQIVQFALLGNMMTLTEFGLVGMITTVTVFAQIVLDMGFGAALIQRDDATERQLSTLYWLNIMTGVLLFVLLYVSSPVIAGFYQREELVFLVRILAIMFLIAPIGQQYQYMLQKQLHFNTLSKIEIFSNVLSFGYLAIAVFMMDAILAYVISQVLLQSSKGILYWAVYRKKWHPAFVFDLRGMKDFFSFGAFQLSSRLVNRLGANIDMILIGRFIGAEALGIYNLAYQIVTIPVLKINPIVTRVAFPIFAKNKYENSVIREGFLNMTKMLALVSFPLLIGLVSVSDAFITAVFGEKWLAAVPILNVLAIVGILRVLMNPNGSVLLAKGRADLAFYWDSGVLLLYGLSLFAAVQTGSLLTVAWVYAIISVVNFLIGRWLLAYVIKLNLSAYFQSIMKPFLITAAMGIIAFGVSLSTEHFSMQAEMRLAISVAAGALCYLFLLVKAYPQTKSKLLRKGRLS"
                     /label="tuaB"
                     /note="Might be involved in the translocation of teichuronic acid repeating units from the inner to the outer surface of the membrane."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCCGTCAAT CACCAAACAG ATCATGTCCG
     2041 GTGCGAAATG GACTAGCATC AGCACCATGT GTATCACGAT CATCCAGATT GTTCAATTTG
     2101 CGCTGCTGGG TAACATGATG ACCCTGACGG AGTTCGGTCT GGTGGGCATG ATCACCACGG
     2161 TGACCGTTTT TGCACAAATC GTCCTGGACA TGGGTTTTGG TGCGGCACTG ATCCAACGTG
     2221 ACGACGCAAC CGAACGTCAG CTGAGCACCC TGTATTGGCT TAACATTATG ACGGGCGTGC
     2281 TGCTGTTTGT GCTGCTGTAC GTGTCCAGCC CGGTTATCGC GGGTTTTTAC CAACGTGAAG
     2341 AATTGGTTTT CCTGGTCCGT ATCCTGGCGA TCATGTTCTT GATCGCGCCG ATTGGTCAGC
     2401 AATACCAATA CATGCTGCAG AAACAGCTGC ACTTCAATAC CCTGTCCAAA ATTGAGATTT
     2461 TTAGCAATGT GCTGTCTTTT GGCTACCTGG CGATTGCCGT GTTCATGATG GATGCGATTC
     2521 TGGCATACGT CATTTCTCAG GTCCTCCTGC AAAGCTCCAA AGGTATTCTG TACTGGGCGG
     2581 TTTATCGTAA GAAATGGCAC CCTGCATTTG TTTTCGACTT GCGTGGTATG AAAGATTTCT
     2641 TCTCCTTCGG TGCATTCCAG CTGAGCAGCC GCCTGGTTAA TCGCCTGGGT GCTAATATCG
     2701 ACATGATCCT GATTGGCCGT TTCATTGGCG CAGAAGCTCT GGGTATCTAC AATCTGGCTT
     2761 ACCAAATCGT GACCATTCCG GTGCTGAAGA TTAATCCGAT TGTCACCCGT GTGGCGTTCC
     2821 CTATCTTCGC GAAGAATAAA TATGAGAATA GCGTGATTCG TGAGGGTTTT CTGAACATGA
     2881 CCAAAATGTT GGCGCTGGTG AGCTTCCCGC TGCTGATTGG TCTGGTGAGT GTCTCCGACG
     2941 CGTTTATCAC CGCTGTCTTT GGTGAAAAAT GGCTGGCCGC GGTGCCGATT CTGAACGTTC
     3001 TGGCGATTGT GGGTATCCTG CGCGTTCTGA TGAATCCTAA TGGTTCCGTT TTGCTGGCCA
     3061 AGGGCCGTGC AGATCTGGCG TTTTATTGGG ATAGCGGCGT GCTGCTGTTG TACGGTCTGA
     3121 GCTTGTTCGC TGCAGTCCAA ACCGGCAGCC TGCTCACGGT TGCATGGGTT TATGCAATTA
     3181 TTAGCGTCGT CAACTTCCTG ATTGGCCGCT GGCTCCTGGC GTATGTGATT AAACTGAATC
     3241 TGAGCGCGTA CTTCCAAAGC ATTATGAAAC CGTTCTTGAT TACCGCGGCC ATGGGCATTA
     3301 TCGCCTTCGG TGTCTCGCTG AGCACGGAAC ACTTCAGCAT GCAAGCGGAG ATGCGCCTGG
     3361 CGATTAGCGT GGCAGCGGGT GCGTTGTGCT ACTTGTTTCT GCTGGTTAAA GCGTACCCGC
     3421 AGACCAAGTC CAAGCTGCTG CGTAAAGGTC GCCTGTCCTG AAGAGCTTAG AGACC
//